Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater
Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, i...
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ftdoajarticles:oai:doaj.org/article:6a9a7a217cfe43ffa384fcf8094cae3c 2023-05-15T13:48:52+02:00 Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad 2021-06-01T00:00:00Z https://doi.org/10.3390/microorganisms9061213 https://doaj.org/article/6a9a7a217cfe43ffa384fcf8094cae3c EN eng MDPI AG https://www.mdpi.com/2076-2607/9/6/1213 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9061213 2076-2607 https://doaj.org/article/6a9a7a217cfe43ffa384fcf8094cae3c Microorganisms, Vol 9, Iss 1213, p 1213 (2021) Antarctica biodegradation diesel microbial consortium seawater Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9061213 2022-12-31T10:00:03Z Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, including the biodegradation of pollutants. However, the native Antarctic microbial diversity provides a reservoir of cold-adapted microorganisms, some of which have the potential for biodegradation. This study evaluated the diesel hydrocarbon-degrading ability of a psychrotolerant marine bacterial consortium obtained from the coast of the north-west Antarctic Peninsula. The consortium’s growth conditions were optimised using one-factor-at-a-time (OFAT) and statistical response surface methodology (RSM), which identified optimal growth conditions of pH 8.0, 10 °C, 25 ppt NaCl and 1.5 g/L NH 4 NO 3 . The predicted model was highly significant and confirmed that the parameters’ salinity, temperature, nitrogen concentration and initial diesel concentration significantly influenced diesel biodegradation. Using the optimised values generated by RSM, a mass reduction of 12.23 mg/mL from the initial 30.518 mg/mL (4% ( w/v )) concentration of diesel was achieved within a 6 d incubation period. This study provides further evidence for the presence of native hydrocarbon-degrading bacteria in non-contaminated Antarctic seawater. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula Microorganisms 9 6 1213 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica biodegradation diesel microbial consortium seawater Biology (General) QH301-705.5 |
spellingShingle |
Antarctica biodegradation diesel microbial consortium seawater Biology (General) QH301-705.5 Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
topic_facet |
Antarctica biodegradation diesel microbial consortium seawater Biology (General) QH301-705.5 |
description |
Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, including the biodegradation of pollutants. However, the native Antarctic microbial diversity provides a reservoir of cold-adapted microorganisms, some of which have the potential for biodegradation. This study evaluated the diesel hydrocarbon-degrading ability of a psychrotolerant marine bacterial consortium obtained from the coast of the north-west Antarctic Peninsula. The consortium’s growth conditions were optimised using one-factor-at-a-time (OFAT) and statistical response surface methodology (RSM), which identified optimal growth conditions of pH 8.0, 10 °C, 25 ppt NaCl and 1.5 g/L NH 4 NO 3 . The predicted model was highly significant and confirmed that the parameters’ salinity, temperature, nitrogen concentration and initial diesel concentration significantly influenced diesel biodegradation. Using the optimised values generated by RSM, a mass reduction of 12.23 mg/mL from the initial 30.518 mg/mL (4% ( w/v )) concentration of diesel was achieved within a 6 d incubation period. This study provides further evidence for the presence of native hydrocarbon-degrading bacteria in non-contaminated Antarctic seawater. |
format |
Article in Journal/Newspaper |
author |
Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad |
author_facet |
Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad |
author_sort |
Nur Nadhirah Zakaria |
title |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_short |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_full |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_fullStr |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_full_unstemmed |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_sort |
statistical optimisation of diesel biodegradation at low temperatures by an antarctic marine bacterial consortium isolated from non-contaminated seawater |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9061213 https://doaj.org/article/6a9a7a217cfe43ffa384fcf8094cae3c |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
Microorganisms, Vol 9, Iss 1213, p 1213 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/6/1213 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9061213 2076-2607 https://doaj.org/article/6a9a7a217cfe43ffa384fcf8094cae3c |
op_doi |
https://doi.org/10.3390/microorganisms9061213 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
6 |
container_start_page |
1213 |
_version_ |
1766249895751057408 |