Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation
Microalgae are well known for their metal sorption capacities, but their potential in the remediation of hydrophobic organic compounds has received little attention in polar regions. We evaluated in the laboratory the ability of an Antarctic microalga to remediate diesel hydrocarbons and also invest...
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2023
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Online Access: | https://doi.org/10.3390/biology12081142 https://doaj.org/article/6a8bf798560d485ab84a0fddf9b3dbba |
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ftdoajarticles:oai:doaj.org/article:6a8bf798560d485ab84a0fddf9b3dbba 2023-09-26T15:11:50+02:00 Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation Zheng Syuen Lim Chiew-Yen Wong Siti Aqlima Ahmad Nurul Aini Puasa Lai Yee Phang Noor Azmi Shaharuddin Faradina Merican Peter Convey Azham Zulkharnain Hasrizal Shaari Alyza Azzura Azmi Yih-Yih Kok Claudio Gomez-Fuentes 2023-08-01T00:00:00Z https://doi.org/10.3390/biology12081142 https://doaj.org/article/6a8bf798560d485ab84a0fddf9b3dbba EN eng MDPI AG https://www.mdpi.com/2079-7737/12/8/1142 https://doaj.org/toc/2079-7737 doi:10.3390/biology12081142 2079-7737 https://doaj.org/article/6a8bf798560d485ab84a0fddf9b3dbba Biology, Vol 12, Iss 1142, p 1142 (2023) Antarctic microalga phytoremediation diesel biodegradation biosorption hydrocarbon Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/biology12081142 2023-08-27T00:36:04Z Microalgae are well known for their metal sorption capacities, but their potential in the remediation of hydrophobic organic compounds has received little attention in polar regions. We evaluated in the laboratory the ability of an Antarctic microalga to remediate diesel hydrocarbons and also investigated physiological changes consequent upon diesel exposure. Using a polyphasic taxonomic approach, the microalgal isolate, WCY_AQ5_1, originally sampled from Greenwich Island (South Shetland Islands, maritime Antarctica) was identified as Tritostichococcus sp. (OQ225631), a recently erected lineage within the redefined Stichococcus clade. Over a nine-day experimental incubation, 57.6% of diesel (~3.47 g/L) was removed via biosorption and biodegradation, demonstrating the strain’s potential for phytoremediation. Fourier transform infrared spectroscopy confirmed the adsorption of oil in accordance with its hydrophobic characteristics. Overall, degradation predominated over sorption of diesel. Chromatographic analysis confirmed that the strain efficiently metabolised medium-chain length n -alkanes (C-7 to C-21), particularly n -heneicosane. Mixotrophic cultivation using diesel as the organic carbon source under a constant light regime altered the car/chl-a ratio and triggered vacuolar activities. A small number of intracellular lipid droplets were observed on the seventh day of cultivation in transmission electron microscopic imaging. This is the first confirmation of diesel remediation ability in an Antarctic green microalga. Article in Journal/Newspaper Antarc* Antarctic Antarctica Greenwich Island South Shetland Islands Directory of Open Access Journals: DOAJ Articles Antarctic Greenwich Greenwich Island ENVELOPE(-59.783,-59.783,-62.517,-62.517) South Shetland Islands Biology 12 8 1142 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic microalga phytoremediation diesel biodegradation biosorption hydrocarbon Biology (General) QH301-705.5 |
spellingShingle |
Antarctic microalga phytoremediation diesel biodegradation biosorption hydrocarbon Biology (General) QH301-705.5 Zheng Syuen Lim Chiew-Yen Wong Siti Aqlima Ahmad Nurul Aini Puasa Lai Yee Phang Noor Azmi Shaharuddin Faradina Merican Peter Convey Azham Zulkharnain Hasrizal Shaari Alyza Azzura Azmi Yih-Yih Kok Claudio Gomez-Fuentes Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation |
topic_facet |
Antarctic microalga phytoremediation diesel biodegradation biosorption hydrocarbon Biology (General) QH301-705.5 |
description |
Microalgae are well known for their metal sorption capacities, but their potential in the remediation of hydrophobic organic compounds has received little attention in polar regions. We evaluated in the laboratory the ability of an Antarctic microalga to remediate diesel hydrocarbons and also investigated physiological changes consequent upon diesel exposure. Using a polyphasic taxonomic approach, the microalgal isolate, WCY_AQ5_1, originally sampled from Greenwich Island (South Shetland Islands, maritime Antarctica) was identified as Tritostichococcus sp. (OQ225631), a recently erected lineage within the redefined Stichococcus clade. Over a nine-day experimental incubation, 57.6% of diesel (~3.47 g/L) was removed via biosorption and biodegradation, demonstrating the strain’s potential for phytoremediation. Fourier transform infrared spectroscopy confirmed the adsorption of oil in accordance with its hydrophobic characteristics. Overall, degradation predominated over sorption of diesel. Chromatographic analysis confirmed that the strain efficiently metabolised medium-chain length n -alkanes (C-7 to C-21), particularly n -heneicosane. Mixotrophic cultivation using diesel as the organic carbon source under a constant light regime altered the car/chl-a ratio and triggered vacuolar activities. A small number of intracellular lipid droplets were observed on the seventh day of cultivation in transmission electron microscopic imaging. This is the first confirmation of diesel remediation ability in an Antarctic green microalga. |
format |
Article in Journal/Newspaper |
author |
Zheng Syuen Lim Chiew-Yen Wong Siti Aqlima Ahmad Nurul Aini Puasa Lai Yee Phang Noor Azmi Shaharuddin Faradina Merican Peter Convey Azham Zulkharnain Hasrizal Shaari Alyza Azzura Azmi Yih-Yih Kok Claudio Gomez-Fuentes |
author_facet |
Zheng Syuen Lim Chiew-Yen Wong Siti Aqlima Ahmad Nurul Aini Puasa Lai Yee Phang Noor Azmi Shaharuddin Faradina Merican Peter Convey Azham Zulkharnain Hasrizal Shaari Alyza Azzura Azmi Yih-Yih Kok Claudio Gomez-Fuentes |
author_sort |
Zheng Syuen Lim |
title |
Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation |
title_short |
Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation |
title_full |
Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation |
title_fullStr |
Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation |
title_full_unstemmed |
Harnessing Diesel-Degrading Potential of an Antarctic Microalga from Greenwich Island and Its Physiological Adaptation |
title_sort |
harnessing diesel-degrading potential of an antarctic microalga from greenwich island and its physiological adaptation |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/biology12081142 https://doaj.org/article/6a8bf798560d485ab84a0fddf9b3dbba |
long_lat |
ENVELOPE(-59.783,-59.783,-62.517,-62.517) |
geographic |
Antarctic Greenwich Greenwich Island South Shetland Islands |
geographic_facet |
Antarctic Greenwich Greenwich Island South Shetland Islands |
genre |
Antarc* Antarctic Antarctica Greenwich Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctica Greenwich Island South Shetland Islands |
op_source |
Biology, Vol 12, Iss 1142, p 1142 (2023) |
op_relation |
https://www.mdpi.com/2079-7737/12/8/1142 https://doaj.org/toc/2079-7737 doi:10.3390/biology12081142 2079-7737 https://doaj.org/article/6a8bf798560d485ab84a0fddf9b3dbba |
op_doi |
https://doi.org/10.3390/biology12081142 |
container_title |
Biology |
container_volume |
12 |
container_issue |
8 |
container_start_page |
1142 |
_version_ |
1778132249972047872 |