Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay
Abstract Background Insecticide-treated nets (ITNs) are losing efficacy against pyrethroid-resistant malaria vector populations throughout Africa. Safeguarding bed net efficacy, vital for effective malaria control, requires greater knowledge of mosquito–ITN interactions and how this impacts on the m...
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ftdoajarticles:oai:doaj.org/article:6a87f0ae28ae42f0b14192cb372345c0 2023-05-15T15:18:37+02:00 Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay Angela Hughes Geraldine M. Foster Amy Guy Agnes Matope Mayumi Abe David Towers Philip J. McCall 2020-04-01T00:00:00Z https://doi.org/10.1186/s12936-020-03213-9 https://doaj.org/article/6a87f0ae28ae42f0b14192cb372345c0 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03213-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03213-9 1475-2875 https://doaj.org/article/6a87f0ae28ae42f0b14192cb372345c0 Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) Mosquito Vector Behaviour Anopheles ITN Pyrethroid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03213-9 2022-12-31T15:45:28Z Abstract Background Insecticide-treated nets (ITNs) are losing efficacy against pyrethroid-resistant malaria vector populations throughout Africa. Safeguarding bed net efficacy, vital for effective malaria control, requires greater knowledge of mosquito–ITN interactions and how this impacts on the mosquito. Methods A purpose-built benchtop apparatus with a closed 10 cm cubic chamber (the ‘Baited-box’) was used to video record behaviour of individual free-flying female Anopheles gambiae during approach and blood-feeding on a human hand through untreated nets and ITNs at close range. Time and duration of defined behavioural events, and knockdown and mortality at 1- and 24-h post-exposure respectively, were recorded for pyrethroid susceptible and resistant mosquitoes. Results Using three human volunteers differing in relative attractiveness to mosquitoes, 328 mosquitoes were individually tested. There were no significant differences between response rates to ITNs and untreated nets (P > 0.1) or between resistant (Tiassalé) and susceptible (Kisumu) mosquito strains, at untreated nets (P = 0.39) or PermaNet 2.0 (P = 1). The sequence of behavioural events from host-seeking to completion of blood-feeding was consistent in all tests but duration and start time of events involving net contact were reduced or delayed respectively with ITNs. Blood-feeding durations at untreated nets (means from 4.25 to 8.47 min (95% confidence interval (CI) = 3.39–9.89) at 3 human volunteers) were reduced by 37–50% at PermaNet 2.0, in susceptible (mean 2.59–4.72 min, 95% CI = 1.54–5.5, P = < 0.05) and resistant (mean 4.20 min, 95% CI = 3.42–4.97, P = 0.01) strains. Total accumulated net contact was approximately 50% lower at PermaNet and Olyset ITNs (P < 0.0001) in susceptible (two of the three volunteers) and resistant mosquitoes. Times prior to first net contact were similar at untreated nets and ITNs (P > 0.2), and neither ITN type showed detectable spatial repellency. After initial contact, blood-feeding commenced later at ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Mosquito Vector Behaviour Anopheles ITN Pyrethroid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Mosquito Vector Behaviour Anopheles ITN Pyrethroid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Angela Hughes Geraldine M. Foster Amy Guy Agnes Matope Mayumi Abe David Towers Philip J. McCall Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
topic_facet |
Mosquito Vector Behaviour Anopheles ITN Pyrethroid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Insecticide-treated nets (ITNs) are losing efficacy against pyrethroid-resistant malaria vector populations throughout Africa. Safeguarding bed net efficacy, vital for effective malaria control, requires greater knowledge of mosquito–ITN interactions and how this impacts on the mosquito. Methods A purpose-built benchtop apparatus with a closed 10 cm cubic chamber (the ‘Baited-box’) was used to video record behaviour of individual free-flying female Anopheles gambiae during approach and blood-feeding on a human hand through untreated nets and ITNs at close range. Time and duration of defined behavioural events, and knockdown and mortality at 1- and 24-h post-exposure respectively, were recorded for pyrethroid susceptible and resistant mosquitoes. Results Using three human volunteers differing in relative attractiveness to mosquitoes, 328 mosquitoes were individually tested. There were no significant differences between response rates to ITNs and untreated nets (P > 0.1) or between resistant (Tiassalé) and susceptible (Kisumu) mosquito strains, at untreated nets (P = 0.39) or PermaNet 2.0 (P = 1). The sequence of behavioural events from host-seeking to completion of blood-feeding was consistent in all tests but duration and start time of events involving net contact were reduced or delayed respectively with ITNs. Blood-feeding durations at untreated nets (means from 4.25 to 8.47 min (95% confidence interval (CI) = 3.39–9.89) at 3 human volunteers) were reduced by 37–50% at PermaNet 2.0, in susceptible (mean 2.59–4.72 min, 95% CI = 1.54–5.5, P = < 0.05) and resistant (mean 4.20 min, 95% CI = 3.42–4.97, P = 0.01) strains. Total accumulated net contact was approximately 50% lower at PermaNet and Olyset ITNs (P < 0.0001) in susceptible (two of the three volunteers) and resistant mosquitoes. Times prior to first net contact were similar at untreated nets and ITNs (P > 0.2), and neither ITN type showed detectable spatial repellency. After initial contact, blood-feeding commenced later at ... |
format |
Article in Journal/Newspaper |
author |
Angela Hughes Geraldine M. Foster Amy Guy Agnes Matope Mayumi Abe David Towers Philip J. McCall |
author_facet |
Angela Hughes Geraldine M. Foster Amy Guy Agnes Matope Mayumi Abe David Towers Philip J. McCall |
author_sort |
Angela Hughes |
title |
Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
title_short |
Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
title_full |
Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
title_fullStr |
Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
title_full_unstemmed |
Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
title_sort |
quantifying late-stage host-seeking behaviour of anopheles gambiae at the insecticidal net interface using a baited-box bioassay |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03213-9 https://doaj.org/article/6a87f0ae28ae42f0b14192cb372345c0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03213-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03213-9 1475-2875 https://doaj.org/article/6a87f0ae28ae42f0b14192cb372345c0 |
op_doi |
https://doi.org/10.1186/s12936-020-03213-9 |
container_title |
Malaria Journal |
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19 |
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1 |
_version_ |
1766348813279166464 |