Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds.
Genome sequencing of Leishmania species that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species sequenced to date, L. major, L. infantum...
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ftdoajarticles:oai:doaj.org/article:6a7f5da7ccb74d0f92a195ebd1b21106 2023-05-15T15:15:35+02:00 Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. Daniel P Depledge Krystal J Evans Alasdair C Ivens Naveed Aziz Asher Maroof Paul M Kaye Deborah F Smith 2009-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000476 https://doaj.org/article/6a7f5da7ccb74d0f92a195ebd1b21106 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2701600?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000476 https://doaj.org/article/6a7f5da7ccb74d0f92a195ebd1b21106 PLoS Neglected Tropical Diseases, Vol 3, Iss 7, p e476 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000476 2022-12-31T01:38:56Z Genome sequencing of Leishmania species that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species sequenced to date, L. major, L. infantum and L. braziliensis. It is not yet known how many of these genes are expressed in the disease-promoting intracellular amastigotes of these species or whether genes conserved between the species are differentially expressed in the host.We have used customised oligonucleotide microarrays to confirm that all of the differentially distributed genes identified by genome comparisons are expressed in intracellular amastigotes, with only a few of these subject to regulation at the RNA level. In the first large-scale study of gene expression in L. braziliensis, we show that only approximately 9% of the genes analysed are regulated in their RNA expression during the L. braziliensis life cycle, a figure consistent with that observed in other Leishmania species. Comparing amastigote gene expression profiles between species confirms the proposal that Leishmania transcriptomes undergo little regulation but also identifies conserved genes that are regulated differently between species in the host. We have also investigated whether host immune competence influences parasite gene expression, by comparing RNA expression profiles in L. major amastigotes derived from either wild-type (BALB/c) or immunologically compromised (Rag2(-/-) gamma(c) (-/-)) mice. While parasite dissemination from the site of infection is enhanced in the Rag2(-/-) gamma(c) (-/-) genetic background, parasite RNA expression profiles are unperturbed.These findings support the hypothesis that Leishmania amastigotes are pre-adapted for intracellular survival and undergo little dynamic modulation of gene expression at the RNA level. Species-specific parasite factors contributing to virulence and pathogenicity in the host may be limited to the products of a small number ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 3 7 e476 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniel P Depledge Krystal J Evans Alasdair C Ivens Naveed Aziz Asher Maroof Paul M Kaye Deborah F Smith Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Genome sequencing of Leishmania species that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species sequenced to date, L. major, L. infantum and L. braziliensis. It is not yet known how many of these genes are expressed in the disease-promoting intracellular amastigotes of these species or whether genes conserved between the species are differentially expressed in the host.We have used customised oligonucleotide microarrays to confirm that all of the differentially distributed genes identified by genome comparisons are expressed in intracellular amastigotes, with only a few of these subject to regulation at the RNA level. In the first large-scale study of gene expression in L. braziliensis, we show that only approximately 9% of the genes analysed are regulated in their RNA expression during the L. braziliensis life cycle, a figure consistent with that observed in other Leishmania species. Comparing amastigote gene expression profiles between species confirms the proposal that Leishmania transcriptomes undergo little regulation but also identifies conserved genes that are regulated differently between species in the host. We have also investigated whether host immune competence influences parasite gene expression, by comparing RNA expression profiles in L. major amastigotes derived from either wild-type (BALB/c) or immunologically compromised (Rag2(-/-) gamma(c) (-/-)) mice. While parasite dissemination from the site of infection is enhanced in the Rag2(-/-) gamma(c) (-/-) genetic background, parasite RNA expression profiles are unperturbed.These findings support the hypothesis that Leishmania amastigotes are pre-adapted for intracellular survival and undergo little dynamic modulation of gene expression at the RNA level. Species-specific parasite factors contributing to virulence and pathogenicity in the host may be limited to the products of a small number ... |
format |
Article in Journal/Newspaper |
author |
Daniel P Depledge Krystal J Evans Alasdair C Ivens Naveed Aziz Asher Maroof Paul M Kaye Deborah F Smith |
author_facet |
Daniel P Depledge Krystal J Evans Alasdair C Ivens Naveed Aziz Asher Maroof Paul M Kaye Deborah F Smith |
author_sort |
Daniel P Depledge |
title |
Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
title_short |
Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
title_full |
Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
title_fullStr |
Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
title_full_unstemmed |
Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
title_sort |
comparative expression profiling of leishmania: modulation in gene expression between species and in different host genetic backgrounds. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000476 https://doaj.org/article/6a7f5da7ccb74d0f92a195ebd1b21106 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 7, p e476 (2009) |
op_relation |
http://europepmc.org/articles/PMC2701600?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000476 https://doaj.org/article/6a7f5da7ccb74d0f92a195ebd1b21106 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000476 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
3 |
container_issue |
7 |
container_start_page |
e476 |
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1766345957275860992 |