Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions
The bioavailability of n -3 long-chain polyunsaturated fatty acids ( n -3 LCPUFAs) has shown to be greatly influenced by their location in the triacylglycerol backbone. Therefore, the synthesis of structured acylglycerols (SAcyl), which include eicosapentaenoic acids (EPAs) or docosahexaenoic acids...
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ftdoajarticles:oai:doaj.org/article:6a50a3c2130748b6977429a8d7a78792 2023-05-15T14:03:42+02:00 Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions Gretel Dovale-Rosabal Alejandra Espinosa Alicia Rodríguez Andrés Barriga Alan Palomino-Calderón Santiago P. Aubourg 2023-02-01T00:00:00Z https://doi.org/10.3390/pr11020537 https://doaj.org/article/6a50a3c2130748b6977429a8d7a78792 EN eng MDPI AG https://www.mdpi.com/2227-9717/11/2/537 https://doaj.org/toc/2227-9717 doi:10.3390/pr11020537 2227-9717 https://doaj.org/article/6a50a3c2130748b6977429a8d7a78792 Processes, Vol 11, Iss 537, p 537 (2023) n -3 LCPUFA EPA/DHA sn -2 position salmon oil immobilized lipase B Candida antarctica Chemical technology TP1-1185 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/pr11020537 2023-02-26T01:28:42Z The bioavailability of n -3 long-chain polyunsaturated fatty acids ( n -3 LCPUFAs) has shown to be greatly influenced by their location in the triacylglycerol backbone. Therefore, the synthesis of structured acylglycerols (SAcyl), which include eicosapentaenoic acids (EPAs) or docosahexaenoic acids (DHAs) at the sn -2 position, has attracted a great interest. The objective of this study was to optimize the synthesis process of a SAcyl from commercial refined salmon oil and an EPA/DHA concentrate in order to enhance the positioning of EPA and DHA in the sn -2 location of the glycerol moiety. For this purpose, immobilized lipase B from Candida antarctica (nonspecific) was used for the acidolysis process under the CO 2 supercritical condition. As a result of carrying out a Draper-Lin composite design through the response surface methodology of 18 experiments, an optimized extraction including SAcyl compounds was obtained. Mass spectrometry (MALDI-TOF) analysis was employed to identify the EPA/DHA location at the sn -2 position in the resulting glycerol moiety. In the fraction obtained, an increase in the EPA and DHA content at the sn -2 position was detected. Remarkably, the optimized SAcyl obtained after 6 h, 82 bar, and 60 °C led to the highest EPA/DHA yield at the sn -2 position in the resulting molecule. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Draper ENVELOPE(-111.252,-111.252,56.667,56.667) Processes 11 2 537 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
n -3 LCPUFA EPA/DHA sn -2 position salmon oil immobilized lipase B Candida antarctica Chemical technology TP1-1185 Chemistry QD1-999 |
spellingShingle |
n -3 LCPUFA EPA/DHA sn -2 position salmon oil immobilized lipase B Candida antarctica Chemical technology TP1-1185 Chemistry QD1-999 Gretel Dovale-Rosabal Alejandra Espinosa Alicia Rodríguez Andrés Barriga Alan Palomino-Calderón Santiago P. Aubourg Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions |
topic_facet |
n -3 LCPUFA EPA/DHA sn -2 position salmon oil immobilized lipase B Candida antarctica Chemical technology TP1-1185 Chemistry QD1-999 |
description |
The bioavailability of n -3 long-chain polyunsaturated fatty acids ( n -3 LCPUFAs) has shown to be greatly influenced by their location in the triacylglycerol backbone. Therefore, the synthesis of structured acylglycerols (SAcyl), which include eicosapentaenoic acids (EPAs) or docosahexaenoic acids (DHAs) at the sn -2 position, has attracted a great interest. The objective of this study was to optimize the synthesis process of a SAcyl from commercial refined salmon oil and an EPA/DHA concentrate in order to enhance the positioning of EPA and DHA in the sn -2 location of the glycerol moiety. For this purpose, immobilized lipase B from Candida antarctica (nonspecific) was used for the acidolysis process under the CO 2 supercritical condition. As a result of carrying out a Draper-Lin composite design through the response surface methodology of 18 experiments, an optimized extraction including SAcyl compounds was obtained. Mass spectrometry (MALDI-TOF) analysis was employed to identify the EPA/DHA location at the sn -2 position in the resulting glycerol moiety. In the fraction obtained, an increase in the EPA and DHA content at the sn -2 position was detected. Remarkably, the optimized SAcyl obtained after 6 h, 82 bar, and 60 °C led to the highest EPA/DHA yield at the sn -2 position in the resulting molecule. |
format |
Article in Journal/Newspaper |
author |
Gretel Dovale-Rosabal Alejandra Espinosa Alicia Rodríguez Andrés Barriga Alan Palomino-Calderón Santiago P. Aubourg |
author_facet |
Gretel Dovale-Rosabal Alejandra Espinosa Alicia Rodríguez Andrés Barriga Alan Palomino-Calderón Santiago P. Aubourg |
author_sort |
Gretel Dovale-Rosabal |
title |
Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions |
title_short |
Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions |
title_full |
Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions |
title_fullStr |
Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions |
title_full_unstemmed |
Enzymatic Synthesis Process of EPA- and DHA-Enriched Structured Acylglycerols at the sn -2 Position Starting from Commercial Salmon Oil and Concentrated by Response Surface Methodology under Supercritical Conditions |
title_sort |
enzymatic synthesis process of epa- and dha-enriched structured acylglycerols at the sn -2 position starting from commercial salmon oil and concentrated by response surface methodology under supercritical conditions |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/pr11020537 https://doaj.org/article/6a50a3c2130748b6977429a8d7a78792 |
long_lat |
ENVELOPE(-111.252,-111.252,56.667,56.667) |
geographic |
Draper |
geographic_facet |
Draper |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Processes, Vol 11, Iss 537, p 537 (2023) |
op_relation |
https://www.mdpi.com/2227-9717/11/2/537 https://doaj.org/toc/2227-9717 doi:10.3390/pr11020537 2227-9717 https://doaj.org/article/6a50a3c2130748b6977429a8d7a78792 |
op_doi |
https://doi.org/10.3390/pr11020537 |
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Processes |
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11 |
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2 |
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537 |
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1766274505959800832 |