Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis
ABSTRACT Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninva...
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ftdoajarticles:oai:doaj.org/article:6a4944c1e56e4ee5ad216fc24319311e 2024-09-09T19:25:36+00:00 Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis Sara Macente Boni Luiza Keiko Oyafuso Rita de Cassia Soler José Angelo Lauletta Lindoso https://doi.org/10.1590/s1678-9946201759038 https://doaj.org/article/6a4944c1e56e4ee5ad216fc24319311e EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000220&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759038 https://doaj.org/article/6a4944c1e56e4ee5ad216fc24319311e Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 Tegumentary Leishmaniasis Polymerase Chain Reaction Swab Leishmania braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946201759038 2024-08-05T17:49:31Z ABSTRACT Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome of the tests performance on swab samples was compatible with PCR results on biopsy samples. The findings have also shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity of the tests and the fact that the sampling method using swabs affords greater patient comfort and safety, it could be said that this method is a promising alternative to conventional biopsy-based methods for the molecular diagnosis of leishmaniasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 59 0 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tegumentary Leishmaniasis Polymerase Chain Reaction Swab Leishmania braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Tegumentary Leishmaniasis Polymerase Chain Reaction Swab Leishmania braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sara Macente Boni Luiza Keiko Oyafuso Rita de Cassia Soler José Angelo Lauletta Lindoso Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis |
topic_facet |
Tegumentary Leishmaniasis Polymerase Chain Reaction Swab Leishmania braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome of the tests performance on swab samples was compatible with PCR results on biopsy samples. The findings have also shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity of the tests and the fact that the sampling method using swabs affords greater patient comfort and safety, it could be said that this method is a promising alternative to conventional biopsy-based methods for the molecular diagnosis of leishmaniasis. |
format |
Article in Journal/Newspaper |
author |
Sara Macente Boni Luiza Keiko Oyafuso Rita de Cassia Soler José Angelo Lauletta Lindoso |
author_facet |
Sara Macente Boni Luiza Keiko Oyafuso Rita de Cassia Soler José Angelo Lauletta Lindoso |
author_sort |
Sara Macente Boni |
title |
Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis |
title_short |
Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis |
title_full |
Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis |
title_fullStr |
Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis |
title_full_unstemmed |
Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis |
title_sort |
efficiency of noninvasive sampling methods (swab) together with polymerase chain reaction (pcr) for diagnosing american tegumentary leishmaniasis |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946201759038 https://doaj.org/article/6a4944c1e56e4ee5ad216fc24319311e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000220&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759038 https://doaj.org/article/6a4944c1e56e4ee5ad216fc24319311e |
op_doi |
https://doi.org/10.1590/s1678-9946201759038 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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59 |
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