Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
ABSTRACT Background: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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Online Access: | https://doi.org/10.1590/0037-8682-0217-2022 https://doaj.org/article/6a3c0557ecf34f649d77118565c783b8 |
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ftdoajarticles:oai:doaj.org/article:6a3c0557ecf34f649d77118565c783b8 2023-05-15T15:00:22+02:00 Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus Blenda Fernandes Emanoelle Fernandes Rutren La Santrer Sônia Maria de Figueiredo Fabiana Rocha-Silva Cláudia Barbosa Assunção Amanda Gabrielle Abreu Iara Furtado Santiago Susana Johann Rachel Basques Caligiorne 2023-03-01T00:00:00Z https://doi.org/10.1590/0037-8682-0217-2022 https://doaj.org/article/6a3c0557ecf34f649d77118565c783b8 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100604&lng=en&tlng=en http://www.scielo.br/pdf/rsbmt/v56/1678-9849-rsbmt-56-e0217-2022.pdf https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0217-2022 https://doaj.org/article/6a3c0557ecf34f649d77118565c783b8 Revista da Sociedade Brasileira de Medicina Tropical, Vol 56 (2023) Sporothrix sp Polymerase chain reaction Sporotrichosis Molecular diagnosis Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1590/0037-8682-0217-2022 2023-03-12T01:33:43Z ABSTRACT Background: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. Results: Three specific primers with 100% specificity for the Sporothrix genus were generated. Conclusions: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 56 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Sporothrix sp Polymerase chain reaction Sporotrichosis Molecular diagnosis Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Sporothrix sp Polymerase chain reaction Sporotrichosis Molecular diagnosis Arctic medicine. Tropical medicine RC955-962 Blenda Fernandes Emanoelle Fernandes Rutren La Santrer Sônia Maria de Figueiredo Fabiana Rocha-Silva Cláudia Barbosa Assunção Amanda Gabrielle Abreu Iara Furtado Santiago Susana Johann Rachel Basques Caligiorne Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
topic_facet |
Sporothrix sp Polymerase chain reaction Sporotrichosis Molecular diagnosis Arctic medicine. Tropical medicine RC955-962 |
description |
ABSTRACT Background: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. Results: Three specific primers with 100% specificity for the Sporothrix genus were generated. Conclusions: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis. |
format |
Article in Journal/Newspaper |
author |
Blenda Fernandes Emanoelle Fernandes Rutren La Santrer Sônia Maria de Figueiredo Fabiana Rocha-Silva Cláudia Barbosa Assunção Amanda Gabrielle Abreu Iara Furtado Santiago Susana Johann Rachel Basques Caligiorne |
author_facet |
Blenda Fernandes Emanoelle Fernandes Rutren La Santrer Sônia Maria de Figueiredo Fabiana Rocha-Silva Cláudia Barbosa Assunção Amanda Gabrielle Abreu Iara Furtado Santiago Susana Johann Rachel Basques Caligiorne |
author_sort |
Blenda Fernandes |
title |
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
title_short |
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
title_full |
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
title_fullStr |
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
title_full_unstemmed |
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
title_sort |
sporotrichosis: in silico design of new molecular markers for the sporothrix genus |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2023 |
url |
https://doi.org/10.1590/0037-8682-0217-2022 https://doaj.org/article/6a3c0557ecf34f649d77118565c783b8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 56 (2023) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100604&lng=en&tlng=en http://www.scielo.br/pdf/rsbmt/v56/1678-9849-rsbmt-56-e0217-2022.pdf https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0217-2022 https://doaj.org/article/6a3c0557ecf34f649d77118565c783b8 |
op_doi |
https://doi.org/10.1590/0037-8682-0217-2022 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
56 |
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