Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border
Abstract Background The aim of this study was to evaluate the clinical outcome after seven-day artesunate monotherapy for uncomplicated Plasmodium falciparum malaria in Yingjiang County along the China-Myanmar border and investigate genetic polymorphisms in the P. falciparum chloroquine-resistance t...
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ftdoajarticles:oai:doaj.org/article:6a19b0f266924a3fb3c0b848173ca2fb 2023-05-15T15:18:39+02:00 Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border Huang Fang Tang Linhua Yang Henglin Zhou Shuisen Sun Xiaodong Liu Hui 2012-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-278 https://doaj.org/article/6a19b0f266924a3fb3c0b848173ca2fb EN eng BMC http://www.malariajournal.com/content/11/1/278 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-278 1475-2875 https://doaj.org/article/6a19b0f266924a3fb3c0b848173ca2fb Malaria Journal, Vol 11, Iss 1, p 278 (2012) Molecular markers Artesunate Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-278 2022-12-30T21:57:31Z Abstract Background The aim of this study was to evaluate the clinical outcome after seven-day artesunate monotherapy for uncomplicated Plasmodium falciparum malaria in Yingjiang County along the China-Myanmar border and investigate genetic polymorphisms in the P. falciparum chloroquine-resistance transporter ( pfcrt ), multidrug resistance 1 ( pfmdr1 ), dihydrofolate reductase ( pfdhfr ), dihydropteroate synthase ( pfdhps ) and ATPase ( pfatp6 ) genes. Methods Patients ≥ one year of age with fever (axillary temperature ≥37.5°C) or history of fever and P. falciparum mono-infection were included. Patients received anti-malarial treatment with artesunate (total dose of 16 mg/kg over seven days) by directly observed therapy. After a 28-day follow-up, treatment efficacy and effectiveness were assessed based on clinical and parasitological outcomes. Treatment failure was defined as recrudescence of the original parasite and distinguished with new infection confirmed by PCR. Analysis of gene mutation and amplification were performed by nested polymerase chain reaction. Results Sixty-five patients were enrolled; 10 withdrew from the study, and six were lost to follow-up. All but two patients demonstrated adequate clinical and parasitological response; 12 had detectable parasitaemia on day 3. These two patients were confirmed to be new infection by PCR. The efficacy of artesunate was 95.9%. The pfcrt mutation in codon 76 was found in all isolates (100%), and mutations in codons 71 and 72 were found in 4.8% of parasite isolates. No mutation of pfmdr1 (codons 86 or 1246) was found. Among all samples, 5.1% were wild type for pfdhfr , whereas the other samples had mutations in four codons (51, 59, 108 and 164), and mutations in pfdhps (codons 436, 437, 540 and 581) were found in all isolates. No samples had mutations in pfatp6 codons 623 or 769, but two new mutations (N683K and R756K) were found in 4.6% and 9.2% of parasite isolates, respectively. Conclusion Plasmodium falciparum infection was associated with slow parasite ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 278 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Molecular markers Artesunate Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Molecular markers Artesunate Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Huang Fang Tang Linhua Yang Henglin Zhou Shuisen Sun Xiaodong Liu Hui Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border |
topic_facet |
Molecular markers Artesunate Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The aim of this study was to evaluate the clinical outcome after seven-day artesunate monotherapy for uncomplicated Plasmodium falciparum malaria in Yingjiang County along the China-Myanmar border and investigate genetic polymorphisms in the P. falciparum chloroquine-resistance transporter ( pfcrt ), multidrug resistance 1 ( pfmdr1 ), dihydrofolate reductase ( pfdhfr ), dihydropteroate synthase ( pfdhps ) and ATPase ( pfatp6 ) genes. Methods Patients ≥ one year of age with fever (axillary temperature ≥37.5°C) or history of fever and P. falciparum mono-infection were included. Patients received anti-malarial treatment with artesunate (total dose of 16 mg/kg over seven days) by directly observed therapy. After a 28-day follow-up, treatment efficacy and effectiveness were assessed based on clinical and parasitological outcomes. Treatment failure was defined as recrudescence of the original parasite and distinguished with new infection confirmed by PCR. Analysis of gene mutation and amplification were performed by nested polymerase chain reaction. Results Sixty-five patients were enrolled; 10 withdrew from the study, and six were lost to follow-up. All but two patients demonstrated adequate clinical and parasitological response; 12 had detectable parasitaemia on day 3. These two patients were confirmed to be new infection by PCR. The efficacy of artesunate was 95.9%. The pfcrt mutation in codon 76 was found in all isolates (100%), and mutations in codons 71 and 72 were found in 4.8% of parasite isolates. No mutation of pfmdr1 (codons 86 or 1246) was found. Among all samples, 5.1% were wild type for pfdhfr , whereas the other samples had mutations in four codons (51, 59, 108 and 164), and mutations in pfdhps (codons 436, 437, 540 and 581) were found in all isolates. No samples had mutations in pfatp6 codons 623 or 769, but two new mutations (N683K and R756K) were found in 4.6% and 9.2% of parasite isolates, respectively. Conclusion Plasmodium falciparum infection was associated with slow parasite ... |
format |
Article in Journal/Newspaper |
author |
Huang Fang Tang Linhua Yang Henglin Zhou Shuisen Sun Xiaodong Liu Hui |
author_facet |
Huang Fang Tang Linhua Yang Henglin Zhou Shuisen Sun Xiaodong Liu Hui |
author_sort |
Huang Fang |
title |
Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border |
title_short |
Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border |
title_full |
Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border |
title_fullStr |
Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border |
title_full_unstemmed |
Therapeutic efficacy of artesunate in the treatment of uncomplicated Plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the China-Myanmar border |
title_sort |
therapeutic efficacy of artesunate in the treatment of uncomplicated plasmodium falciparum malaria and anti-malarial, drug-resistance marker polymorphisms in populations near the china-myanmar border |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-278 https://doaj.org/article/6a19b0f266924a3fb3c0b848173ca2fb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 278 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/278 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-278 1475-2875 https://doaj.org/article/6a19b0f266924a3fb3c0b848173ca2fb |
op_doi |
https://doi.org/10.1186/1475-2875-11-278 |
container_title |
Malaria Journal |
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11 |
container_issue |
1 |
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278 |
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1766348846675263488 |