Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow?
The effect of nutrients on microbial interactions, including competition and collaboration, has mainly been studied in laboratories, but their potential application to complex ecosystems is unknown. Here, we examined the effect of changes in organic acids among other parameters on snow microbial com...
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Online Access: | https://doi.org/10.3389/fmicb.2019.02492 https://doaj.org/article/69e7fc4a503c45d2aef775359cfa66f9 |
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ftdoajarticles:oai:doaj.org/article:69e7fc4a503c45d2aef775359cfa66f9 2023-05-15T14:59:55+02:00 Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? Benoît Bergk Pinto Lorrie Maccario Aurélien Dommergue Timothy M. Vogel Catherine Larose 2019-10-01T00:00:00Z https://doi.org/10.3389/fmicb.2019.02492 https://doaj.org/article/69e7fc4a503c45d2aef775359cfa66f9 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2019.02492/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.02492 https://doaj.org/article/69e7fc4a503c45d2aef775359cfa66f9 Frontiers in Microbiology, Vol 10 (2019) competition cooperation networks snow organic acids Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.3389/fmicb.2019.02492 2022-12-31T11:38:54Z The effect of nutrients on microbial interactions, including competition and collaboration, has mainly been studied in laboratories, but their potential application to complex ecosystems is unknown. Here, we examined the effect of changes in organic acids among other parameters on snow microbial communities in situ over 2 months. We compared snow bacterial communities from a low organic acid content period to that from a higher organic acid period. We hypothesized that an increase in organic acids would shift the dominant microbial interaction from collaboration to competition. To evaluate microbial interactions, we built taxonomic co-variance networks from OTUs obtained from 16S rRNA gene sequencing. In addition, we tracked marker genes of microbial cooperation (plasmid backbone genes) and competition (antibiotic resistance genes) across both sampling periods in metagenomes and metatranscriptomes. Our results showed a decrease in the average connectivity of the network during late spring compared to the early spring that we interpreted as a decrease of cooperation. This observation was strengthened by the significantly more abundant plasmid backbone genes in the metagenomes from the early spring. The modularity of the network from the late spring was also found to be higher than the one from the early spring, which is another possible indicator of increased competition. Antibiotic resistance genes were significantly more abundant in the late spring metagenomes. In addition, antibiotic resistance genes were also positively correlated to the organic acid concentration of the snow across both seasons. Snow organic acid content might be responsible for this change in bacterial interactions in the Arctic snow community. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
competition cooperation networks snow organic acids Microbiology QR1-502 |
spellingShingle |
competition cooperation networks snow organic acids Microbiology QR1-502 Benoît Bergk Pinto Lorrie Maccario Aurélien Dommergue Timothy M. Vogel Catherine Larose Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
topic_facet |
competition cooperation networks snow organic acids Microbiology QR1-502 |
description |
The effect of nutrients on microbial interactions, including competition and collaboration, has mainly been studied in laboratories, but their potential application to complex ecosystems is unknown. Here, we examined the effect of changes in organic acids among other parameters on snow microbial communities in situ over 2 months. We compared snow bacterial communities from a low organic acid content period to that from a higher organic acid period. We hypothesized that an increase in organic acids would shift the dominant microbial interaction from collaboration to competition. To evaluate microbial interactions, we built taxonomic co-variance networks from OTUs obtained from 16S rRNA gene sequencing. In addition, we tracked marker genes of microbial cooperation (plasmid backbone genes) and competition (antibiotic resistance genes) across both sampling periods in metagenomes and metatranscriptomes. Our results showed a decrease in the average connectivity of the network during late spring compared to the early spring that we interpreted as a decrease of cooperation. This observation was strengthened by the significantly more abundant plasmid backbone genes in the metagenomes from the early spring. The modularity of the network from the late spring was also found to be higher than the one from the early spring, which is another possible indicator of increased competition. Antibiotic resistance genes were significantly more abundant in the late spring metagenomes. In addition, antibiotic resistance genes were also positively correlated to the organic acid concentration of the snow across both seasons. Snow organic acid content might be responsible for this change in bacterial interactions in the Arctic snow community. |
format |
Article in Journal/Newspaper |
author |
Benoît Bergk Pinto Lorrie Maccario Aurélien Dommergue Timothy M. Vogel Catherine Larose |
author_facet |
Benoît Bergk Pinto Lorrie Maccario Aurélien Dommergue Timothy M. Vogel Catherine Larose |
author_sort |
Benoît Bergk Pinto |
title |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_short |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_full |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_fullStr |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_full_unstemmed |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_sort |
do organic substrates drive microbial community interactions in arctic snow? |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.02492 https://doaj.org/article/69e7fc4a503c45d2aef775359cfa66f9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Frontiers in Microbiology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02492/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.02492 https://doaj.org/article/69e7fc4a503c45d2aef775359cfa66f9 |
op_doi |
https://doi.org/10.3389/fmicb.2019.02492 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766332027432337408 |