Identification and localization of the structural proteins of anguillid herpesvirus 1
Abstract Many of the known fish herpesviruses have important aquaculture species as their natural host, and may cause serious disease and mortality. Anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in European eel, Anguilla anguilla . Despite their importance, fundamental molecular kno...
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ftdoajarticles:oai:doaj.org/article:69dab4a044a544cca563b92cd87ed776 2023-05-15T13:27:54+02:00 Identification and localization of the structural proteins of anguillid herpesvirus 1 van Beurden Steven J Leroy Baptiste Wattiez Ruddy Haenen Olga LM Boeren Sjef Vervoort Jacques JM Peeters Ben PH Rottier Peter JM Engelsma Marc Y Vanderplasschen Alain F 2011-10-01T00:00:00Z https://doi.org/10.1186/1297-9716-42-105 https://doaj.org/article/69dab4a044a544cca563b92cd87ed776 EN eng BMC http://www.veterinaryresearch.org/content/42/1/105 https://doaj.org/toc/0928-4249 https://doaj.org/toc/1297-9716 doi:10.1186/1297-9716-42-105 0928-4249 1297-9716 https://doaj.org/article/69dab4a044a544cca563b92cd87ed776 Veterinary Research, Vol 42, Iss 1, p 105 (2011) Veterinary medicine SF600-1100 article 2011 ftdoajarticles https://doi.org/10.1186/1297-9716-42-105 2022-12-31T11:46:17Z Abstract Many of the known fish herpesviruses have important aquaculture species as their natural host, and may cause serious disease and mortality. Anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in European eel, Anguilla anguilla . Despite their importance, fundamental molecular knowledge on fish herpesviruses is still limited. In this study we describe the identification and localization of the structural proteins of AngHV-1. Purified virions were fractionated into a capsid-tegument and an envelope fraction, and premature capsids were isolated from infected cells. Proteins were extracted by different methods and identified by mass spectrometry. A total of 40 structural proteins were identified, of which 7 could be assigned to the capsid, 11 to the envelope, and 22 to the tegument. The identification and localization of these proteins allowed functional predictions. Our findings include the identification of the putative capsid triplex protein 1, the predominant tegument protein, and the major antigenic envelope proteins. Eighteen of the 40 AngHV-1 structural proteins had sequence homologues in related Cyprinid herpesvirus 3 (CyHV-3). Conservation of fish herpesvirus structural genes seemed to be high for the capsid proteins, limited for the tegument proteins, and low for the envelope proteins. The identification and localization of the structural proteins of AngHV-1 in this study adds to the fundamental knowledge of members of the Alloherpesviridae family, especially of the Cyprinivirus genus. Article in Journal/Newspaper Anguilla anguilla European eel Directory of Open Access Journals: DOAJ Articles Veterinary Research 42 1 105 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Veterinary medicine SF600-1100 |
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Veterinary medicine SF600-1100 van Beurden Steven J Leroy Baptiste Wattiez Ruddy Haenen Olga LM Boeren Sjef Vervoort Jacques JM Peeters Ben PH Rottier Peter JM Engelsma Marc Y Vanderplasschen Alain F Identification and localization of the structural proteins of anguillid herpesvirus 1 |
topic_facet |
Veterinary medicine SF600-1100 |
description |
Abstract Many of the known fish herpesviruses have important aquaculture species as their natural host, and may cause serious disease and mortality. Anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in European eel, Anguilla anguilla . Despite their importance, fundamental molecular knowledge on fish herpesviruses is still limited. In this study we describe the identification and localization of the structural proteins of AngHV-1. Purified virions were fractionated into a capsid-tegument and an envelope fraction, and premature capsids were isolated from infected cells. Proteins were extracted by different methods and identified by mass spectrometry. A total of 40 structural proteins were identified, of which 7 could be assigned to the capsid, 11 to the envelope, and 22 to the tegument. The identification and localization of these proteins allowed functional predictions. Our findings include the identification of the putative capsid triplex protein 1, the predominant tegument protein, and the major antigenic envelope proteins. Eighteen of the 40 AngHV-1 structural proteins had sequence homologues in related Cyprinid herpesvirus 3 (CyHV-3). Conservation of fish herpesvirus structural genes seemed to be high for the capsid proteins, limited for the tegument proteins, and low for the envelope proteins. The identification and localization of the structural proteins of AngHV-1 in this study adds to the fundamental knowledge of members of the Alloherpesviridae family, especially of the Cyprinivirus genus. |
format |
Article in Journal/Newspaper |
author |
van Beurden Steven J Leroy Baptiste Wattiez Ruddy Haenen Olga LM Boeren Sjef Vervoort Jacques JM Peeters Ben PH Rottier Peter JM Engelsma Marc Y Vanderplasschen Alain F |
author_facet |
van Beurden Steven J Leroy Baptiste Wattiez Ruddy Haenen Olga LM Boeren Sjef Vervoort Jacques JM Peeters Ben PH Rottier Peter JM Engelsma Marc Y Vanderplasschen Alain F |
author_sort |
van Beurden Steven J |
title |
Identification and localization of the structural proteins of anguillid herpesvirus 1 |
title_short |
Identification and localization of the structural proteins of anguillid herpesvirus 1 |
title_full |
Identification and localization of the structural proteins of anguillid herpesvirus 1 |
title_fullStr |
Identification and localization of the structural proteins of anguillid herpesvirus 1 |
title_full_unstemmed |
Identification and localization of the structural proteins of anguillid herpesvirus 1 |
title_sort |
identification and localization of the structural proteins of anguillid herpesvirus 1 |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1297-9716-42-105 https://doaj.org/article/69dab4a044a544cca563b92cd87ed776 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Veterinary Research, Vol 42, Iss 1, p 105 (2011) |
op_relation |
http://www.veterinaryresearch.org/content/42/1/105 https://doaj.org/toc/0928-4249 https://doaj.org/toc/1297-9716 doi:10.1186/1297-9716-42-105 0928-4249 1297-9716 https://doaj.org/article/69dab4a044a544cca563b92cd87ed776 |
op_doi |
https://doi.org/10.1186/1297-9716-42-105 |
container_title |
Veterinary Research |
container_volume |
42 |
container_issue |
1 |
container_start_page |
105 |
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1766401020753084416 |