Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding
We investigated the possibility of bacterial symbiosis in Globigerina bulloides , a palaeoceanographically important, planktonic foraminifer. This marine protist is commonly used in micropalaeontological investigations of climatically sensitive subpolar and temperate water masses as well as wind-dri...
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ftdoajarticles:oai:doaj.org/article:6986299de4cb4a8ea818f62ae737ffbc 2023-05-15T17:52:00+02:00 Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding C. Bird K. F. Darling A. D. Russell C. V. Davis J. Fehrenbacher A. Free M. Wyman B. T. Ngwenya 2017-02-01T00:00:00Z https://doi.org/10.5194/bg-14-901-2017 https://doaj.org/article/6986299de4cb4a8ea818f62ae737ffbc EN eng Copernicus Publications http://www.biogeosciences.net/14/901/2017/bg-14-901-2017.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-14-901-2017 https://doaj.org/article/6986299de4cb4a8ea818f62ae737ffbc Biogeosciences, Vol 14, Iss 4, Pp 901-920 (2017) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2017 ftdoajarticles https://doi.org/10.5194/bg-14-901-2017 2022-12-31T14:40:57Z We investigated the possibility of bacterial symbiosis in Globigerina bulloides , a palaeoceanographically important, planktonic foraminifer. This marine protist is commonly used in micropalaeontological investigations of climatically sensitive subpolar and temperate water masses as well as wind-driven upwelling regions of the world's oceans. G. bulloides is unusual because it lacks the protist algal symbionts that are often found in other spinose species. In addition, it has a large offset in its stable carbon and oxygen isotopic compositions compared to other planktonic foraminifer species, and also that predicted from seawater equilibrium. This is suggestive of novel differences in ecology and life history of G. bulloides , making it a good candidate for investigating the potential for bacterial symbiosis as a contributory factor influencing shell calcification. Such information is essential to evaluate fully the potential response of G. bulloides to ocean acidification and climate change. To investigate possible ecological interactions between G. bulloides and marine bacteria, 18S rRNA gene sequencing, fluorescence microscopy, 16S rRNA gene metabarcoding and transmission electron microscopy (TEM) were performed on individual specimens of G. bulloides (type IId) collected from two locations in the California Current. Intracellular DNA extracted from five G. bulloides specimens was subjected to 16S rRNA gene metabarcoding and, remarkably, 37–87 % of all 16S rRNA gene sequences recovered were assigned to operational taxonomic units (OTUs) from the picocyanobacterium Synechococcus . This finding was supported by TEM observations of intact Synechococcus cells in both the cytoplasm and vacuoles of G. bulloides . Their concentrations were up to 4 orders of magnitude greater inside the foraminifera than those reported for the California Current water column and approximately 5 % of the intracellular Synechococcus cells observed were undergoing cell division. This suggests that Synechococcus is an endobiont of G. ... Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 14 4 901 920 |
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English |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 C. Bird K. F. Darling A. D. Russell C. V. Davis J. Fehrenbacher A. Free M. Wyman B. T. Ngwenya Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
We investigated the possibility of bacterial symbiosis in Globigerina bulloides , a palaeoceanographically important, planktonic foraminifer. This marine protist is commonly used in micropalaeontological investigations of climatically sensitive subpolar and temperate water masses as well as wind-driven upwelling regions of the world's oceans. G. bulloides is unusual because it lacks the protist algal symbionts that are often found in other spinose species. In addition, it has a large offset in its stable carbon and oxygen isotopic compositions compared to other planktonic foraminifer species, and also that predicted from seawater equilibrium. This is suggestive of novel differences in ecology and life history of G. bulloides , making it a good candidate for investigating the potential for bacterial symbiosis as a contributory factor influencing shell calcification. Such information is essential to evaluate fully the potential response of G. bulloides to ocean acidification and climate change. To investigate possible ecological interactions between G. bulloides and marine bacteria, 18S rRNA gene sequencing, fluorescence microscopy, 16S rRNA gene metabarcoding and transmission electron microscopy (TEM) were performed on individual specimens of G. bulloides (type IId) collected from two locations in the California Current. Intracellular DNA extracted from five G. bulloides specimens was subjected to 16S rRNA gene metabarcoding and, remarkably, 37–87 % of all 16S rRNA gene sequences recovered were assigned to operational taxonomic units (OTUs) from the picocyanobacterium Synechococcus . This finding was supported by TEM observations of intact Synechococcus cells in both the cytoplasm and vacuoles of G. bulloides . Their concentrations were up to 4 orders of magnitude greater inside the foraminifera than those reported for the California Current water column and approximately 5 % of the intracellular Synechococcus cells observed were undergoing cell division. This suggests that Synechococcus is an endobiont of G. ... |
format |
Article in Journal/Newspaper |
author |
C. Bird K. F. Darling A. D. Russell C. V. Davis J. Fehrenbacher A. Free M. Wyman B. T. Ngwenya |
author_facet |
C. Bird K. F. Darling A. D. Russell C. V. Davis J. Fehrenbacher A. Free M. Wyman B. T. Ngwenya |
author_sort |
C. Bird |
title |
Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding |
title_short |
Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding |
title_full |
Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding |
title_fullStr |
Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding |
title_full_unstemmed |
Cyanobacterial endobionts within a major marine planktonic calcifier ( Globigerina bulloides , Foraminifera) revealed by 16S rRNA metabarcoding |
title_sort |
cyanobacterial endobionts within a major marine planktonic calcifier ( globigerina bulloides , foraminifera) revealed by 16s rrna metabarcoding |
publisher |
Copernicus Publications |
publishDate |
2017 |
url |
https://doi.org/10.5194/bg-14-901-2017 https://doaj.org/article/6986299de4cb4a8ea818f62ae737ffbc |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 14, Iss 4, Pp 901-920 (2017) |
op_relation |
http://www.biogeosciences.net/14/901/2017/bg-14-901-2017.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-14-901-2017 https://doaj.org/article/6986299de4cb4a8ea818f62ae737ffbc |
op_doi |
https://doi.org/10.5194/bg-14-901-2017 |
container_title |
Biogeosciences |
container_volume |
14 |
container_issue |
4 |
container_start_page |
901 |
op_container_end_page |
920 |
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1766159308060360704 |