Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo 6+ ) to a less toxic compound, Mo-blue, using microorganisms provides a sus...
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ftdoajarticles:oai:doaj.org/article:6980db607e034930b1c30e1ba2cd69e2 2023-05-15T13:45:27+02:00 Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad 2021-06-01T00:00:00Z https://doi.org/10.3390/jmse9060648 https://doaj.org/article/6980db607e034930b1c30e1ba2cd69e2 EN eng MDPI AG https://www.mdpi.com/2077-1312/9/6/648 https://doaj.org/toc/2077-1312 doi:10.3390/jmse9060648 2077-1312 https://doaj.org/article/6980db607e034930b1c30e1ba2cd69e2 Journal of Marine Science and Engineering, Vol 9, Iss 648, p 648 (2021) Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 article 2021 ftdoajarticles https://doi.org/10.3390/jmse9060648 2022-12-31T09:54:35Z The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo 6+ ) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate ( µ max ) was 0.067 h −1 . The data obtained support the potential use of marine bacteria in the bioremediation of Mo. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Journal of Marine Science and Engineering 9 6 648 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 |
spellingShingle |
Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
topic_facet |
Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 |
description |
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo 6+ ) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate ( µ max ) was 0.067 h −1 . The data obtained support the potential use of marine bacteria in the bioremediation of Mo. |
format |
Article in Journal/Newspaper |
author |
Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad |
author_facet |
Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad |
author_sort |
Syazani Darham |
title |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_short |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_full |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_fullStr |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_full_unstemmed |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_sort |
statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from antarctica |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/jmse9060648 https://doaj.org/article/6980db607e034930b1c30e1ba2cd69e2 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Journal of Marine Science and Engineering, Vol 9, Iss 648, p 648 (2021) |
op_relation |
https://www.mdpi.com/2077-1312/9/6/648 https://doaj.org/toc/2077-1312 doi:10.3390/jmse9060648 2077-1312 https://doaj.org/article/6980db607e034930b1c30e1ba2cd69e2 |
op_doi |
https://doi.org/10.3390/jmse9060648 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
9 |
container_issue |
6 |
container_start_page |
648 |
_version_ |
1766224642747400192 |