South american rattlesnake venom: its hemolytic power
The hemolytic power of rattlesnake venom (Crotalus durissus terrificus) was Studied. A high percentage of sample with negative hemolytic power was detected when sheep red blood cells were used. A large number of venoms with hemolytic power, though with a low hemolysis percentage, were detected when...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1989
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Online Access: | https://doi.org/10.1590/S0037-86821989000400002 https://doaj.org/article/6975c7f1ac5d4e24b6d6fb07483b9480 |
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ftdoajarticles:oai:doaj.org/article:6975c7f1ac5d4e24b6d6fb07483b9480 2023-05-15T15:08:22+02:00 South american rattlesnake venom: its hemolytic power Édimo Garcia de Lima Paulo Inácio da Costa Carlos Julio Laure 1989-12-01T00:00:00Z https://doi.org/10.1590/S0037-86821989000400002 https://doaj.org/article/6975c7f1ac5d4e24b6d6fb07483b9480 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000400002&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86821989000400002 https://doaj.org/article/6975c7f1ac5d4e24b6d6fb07483b9480 Revista da Sociedade Brasileira de Medicina Tropical, Vol 22, Iss 4, Pp 171-175 (1989) Veneno de cascavel Poder hemolítico Arctic medicine. Tropical medicine RC955-962 article 1989 ftdoajarticles https://doi.org/10.1590/S0037-86821989000400002 2022-12-30T21:27:33Z The hemolytic power of rattlesnake venom (Crotalus durissus terrificus) was Studied. A high percentage of sample with negative hemolytic power was detected when sheep red blood cells were used. A large number of venoms with hemolytic power, though with a low hemolysis percentage, were detected when liquid, recently extracted venom was used. When crystallized venom was used under the same experimental conditions, a higher percentage ofpositivityfor hemolysis was obtained. When the results obtained on agar plates were compared to those obtained in test tubes, a large number of animals with a higher percentage of hemolysis were detected, though this value was not proportional to the number of animals showing positive plate hemolysis. When the hemolytic power of these venoms was tested on human red blood cells, a large percentage of animals with venoms having a low hemolytic power was also detected. Hemolytic power was much greater when human red blood cells were tested with crystallized venom. The preparation of red blood cells also had an important effect and the use of red blood cells from defibrinated blood is recommended. We conclude that rattlesnake venom has hemolytic power that increases when the venom is crystallized. Red blood cells should be properly preparedfor the lysis reactions. We suggest that the lytic power of the venom is related to venom concentration and to the purity of its fractions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 22 4 171 175 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Veneno de cascavel Poder hemolítico Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Veneno de cascavel Poder hemolítico Arctic medicine. Tropical medicine RC955-962 Édimo Garcia de Lima Paulo Inácio da Costa Carlos Julio Laure South american rattlesnake venom: its hemolytic power |
topic_facet |
Veneno de cascavel Poder hemolítico Arctic medicine. Tropical medicine RC955-962 |
description |
The hemolytic power of rattlesnake venom (Crotalus durissus terrificus) was Studied. A high percentage of sample with negative hemolytic power was detected when sheep red blood cells were used. A large number of venoms with hemolytic power, though with a low hemolysis percentage, were detected when liquid, recently extracted venom was used. When crystallized venom was used under the same experimental conditions, a higher percentage ofpositivityfor hemolysis was obtained. When the results obtained on agar plates were compared to those obtained in test tubes, a large number of animals with a higher percentage of hemolysis were detected, though this value was not proportional to the number of animals showing positive plate hemolysis. When the hemolytic power of these venoms was tested on human red blood cells, a large percentage of animals with venoms having a low hemolytic power was also detected. Hemolytic power was much greater when human red blood cells were tested with crystallized venom. The preparation of red blood cells also had an important effect and the use of red blood cells from defibrinated blood is recommended. We conclude that rattlesnake venom has hemolytic power that increases when the venom is crystallized. Red blood cells should be properly preparedfor the lysis reactions. We suggest that the lytic power of the venom is related to venom concentration and to the purity of its fractions. |
format |
Article in Journal/Newspaper |
author |
Édimo Garcia de Lima Paulo Inácio da Costa Carlos Julio Laure |
author_facet |
Édimo Garcia de Lima Paulo Inácio da Costa Carlos Julio Laure |
author_sort |
Édimo Garcia de Lima |
title |
South american rattlesnake venom: its hemolytic power |
title_short |
South american rattlesnake venom: its hemolytic power |
title_full |
South american rattlesnake venom: its hemolytic power |
title_fullStr |
South american rattlesnake venom: its hemolytic power |
title_full_unstemmed |
South american rattlesnake venom: its hemolytic power |
title_sort |
south american rattlesnake venom: its hemolytic power |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1989 |
url |
https://doi.org/10.1590/S0037-86821989000400002 https://doaj.org/article/6975c7f1ac5d4e24b6d6fb07483b9480 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 22, Iss 4, Pp 171-175 (1989) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000400002&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86821989000400002 https://doaj.org/article/6975c7f1ac5d4e24b6d6fb07483b9480 |
op_doi |
https://doi.org/10.1590/S0037-86821989000400002 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
22 |
container_issue |
4 |
container_start_page |
171 |
op_container_end_page |
175 |
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