Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica
Warming in the Antarctic Peninsula is one of the fastest on earth, and is predicted to become more asymmetric in the near future. Warming has already favored the growth and reproduction of Antarctic plant species, leading to a decrease in their freezing tolerance (deacclimation). Evidence regarding...
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ftdoajarticles:oai:doaj.org/article:69340cdaf6fd48229164b90473c07c60 2023-07-30T03:56:36+02:00 Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica Dariel López Giovanni Larama Patricia L. Sáez León A. Bravo 2023-07-01T00:00:00Z https://doi.org/10.3390/ijms241311211 https://doaj.org/article/69340cdaf6fd48229164b90473c07c60 EN eng MDPI AG https://www.mdpi.com/1422-0067/24/13/11211 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241311211 1422-0067 1661-6596 https://doaj.org/article/69340cdaf6fd48229164b90473c07c60 International Journal of Molecular Sciences, Vol 24, Iss 11211, p 11211 (2023) Antarctic plant asymmetric warming CBF climate change cold deacclimation gene expression Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241311211 2023-07-16T00:35:04Z Warming in the Antarctic Peninsula is one of the fastest on earth, and is predicted to become more asymmetric in the near future. Warming has already favored the growth and reproduction of Antarctic plant species, leading to a decrease in their freezing tolerance (deacclimation). Evidence regarding the effects of diurnal and nocturnal warming on freezing tolerance-related gene expression in D. antarctica is negligible. We hypothesized that freezing tolerance-related gene (such as CBF-regulon) expression is reduced mainly by nocturnal warming rather than diurnal temperature changes in D. antarctica. The present work aimed to determine the effects of diurnal and nocturnal warming on cold deacclimation and its associated gene expression in D. antarctica , under laboratory conditions. Fully cold-acclimated plants (8 °C/0 °C), with 16h/8h thermoperiod and photoperiod duration, were assigned to four treatments for 14 days: one control (8 °C/0 °C) and three with different warming conditions (diurnal (14 °C/0 °C), nocturnal (8 °C/6 °C), and diurnal-nocturnal (14 °C/6 °C). RNA-seq was performed and differential gene expression was analyzed. Nocturnal warming significantly down-regulated the CBF transcription factors expression and associated cold stress response genes and up-regulated photosynthetic and growth promotion genes. Consequently, nocturnal warming has a greater effect than diurnal warming on the cold deacclimation process in D. antarctica . The eco-physiological implications are discussed. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula The Antarctic International Journal of Molecular Sciences 24 13 11211 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic plant asymmetric warming CBF climate change cold deacclimation gene expression Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Antarctic plant asymmetric warming CBF climate change cold deacclimation gene expression Biology (General) QH301-705.5 Chemistry QD1-999 Dariel López Giovanni Larama Patricia L. Sáez León A. Bravo Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica |
topic_facet |
Antarctic plant asymmetric warming CBF climate change cold deacclimation gene expression Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Warming in the Antarctic Peninsula is one of the fastest on earth, and is predicted to become more asymmetric in the near future. Warming has already favored the growth and reproduction of Antarctic plant species, leading to a decrease in their freezing tolerance (deacclimation). Evidence regarding the effects of diurnal and nocturnal warming on freezing tolerance-related gene expression in D. antarctica is negligible. We hypothesized that freezing tolerance-related gene (such as CBF-regulon) expression is reduced mainly by nocturnal warming rather than diurnal temperature changes in D. antarctica. The present work aimed to determine the effects of diurnal and nocturnal warming on cold deacclimation and its associated gene expression in D. antarctica , under laboratory conditions. Fully cold-acclimated plants (8 °C/0 °C), with 16h/8h thermoperiod and photoperiod duration, were assigned to four treatments for 14 days: one control (8 °C/0 °C) and three with different warming conditions (diurnal (14 °C/0 °C), nocturnal (8 °C/6 °C), and diurnal-nocturnal (14 °C/6 °C). RNA-seq was performed and differential gene expression was analyzed. Nocturnal warming significantly down-regulated the CBF transcription factors expression and associated cold stress response genes and up-regulated photosynthetic and growth promotion genes. Consequently, nocturnal warming has a greater effect than diurnal warming on the cold deacclimation process in D. antarctica . The eco-physiological implications are discussed. |
format |
Article in Journal/Newspaper |
author |
Dariel López Giovanni Larama Patricia L. Sáez León A. Bravo |
author_facet |
Dariel López Giovanni Larama Patricia L. Sáez León A. Bravo |
author_sort |
Dariel López |
title |
Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica |
title_short |
Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica |
title_full |
Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica |
title_fullStr |
Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica |
title_full_unstemmed |
Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica |
title_sort |
transcriptome analysis of diurnal and nocturnal-warmed plants, the molecular mechanism underlying cold deacclimation response in deschampsia antarctica |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241311211 https://doaj.org/article/69340cdaf6fd48229164b90473c07c60 |
geographic |
Antarctic Antarctic Peninsula The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 11211, p 11211 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/13/11211 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241311211 1422-0067 1661-6596 https://doaj.org/article/69340cdaf6fd48229164b90473c07c60 |
op_doi |
https://doi.org/10.3390/ijms241311211 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
13 |
container_start_page |
11211 |
_version_ |
1772813811737165824 |