Sexual recombination is a signature of a persisting malaria epidemic in Peru
Abstract Background The aim of this study was to consider the impact that multi-clone, complex infections have on a parasite population structure in a low transmission setting. In general, complexity of infection (minimum number of clones within an infection) and the overall population level diversi...
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ftdoajarticles:oai:doaj.org/article:690ac946148340c8a17f502bed232578 2023-05-15T15:14:28+02:00 Sexual recombination is a signature of a persisting malaria epidemic in Peru Sutton Patrick L Torres Lindsay P Branch OraLee H 2011-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-329 https://doaj.org/article/690ac946148340c8a17f502bed232578 EN eng BMC http://www.malariajournal.com/content/10/1/329 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-329 1475-2875 https://doaj.org/article/690ac946148340c8a17f502bed232578 Malaria Journal, Vol 10, Iss 1, p 329 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-329 2022-12-31T00:40:01Z Abstract Background The aim of this study was to consider the impact that multi-clone, complex infections have on a parasite population structure in a low transmission setting. In general, complexity of infection (minimum number of clones within an infection) and the overall population level diversity is expected to be minimal in low transmission settings. Additionally, the parasite population structure is predicted to be clonal, rather than sexual due to infrequent parasite inoculation and lack of recombination between genetically distinct clones. However, in this low transmission of the Peruvian Amazon, complex infections are becoming more frequent, in spite of decreasing infection prevalence. In this study, it was hypothesized that sexual recombination between distinct clonal lineages of Plasmodium falciparum parasites were altering the subpopulation structure and effectively maintaining the population-level diversity. Methods Fourteen microsatellite markers were chosen to describe the genetic diversity in 313 naturally occurring P. falciparum infections from Peruvian Amazon. The population and subpopulation structure was characterized by measuring: clusteredness, expected heterozygosity (H e ), allelic richness, private allelic richness, and linkage disequilibrium. Next, microsatellite haplotypes and alleles were correlated with P. falciparum merozoite surface protein 1 Block 2 ( Pf msp1-B2) to examine the presence of recombinant microsatellite haplotypes. Results The parasite population structure consists of six genetically diverse subpopulations of clones, called "clusters". Clusters 1, 3, 4, and 6 have unique haplotypes that exceed 70% of the total number of clones within each cluster, while Clusters 2 and 5 have a lower proportion of unique haplotypes, but still exceed 46%. By measuring the H e , allelic richness, and private allelic richness within each of the six subpopulations, relatively low levels of genetic diversity within each subpopulation (except Cluster 4) are observed. This indicated that the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 329 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sutton Patrick L Torres Lindsay P Branch OraLee H Sexual recombination is a signature of a persisting malaria epidemic in Peru |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The aim of this study was to consider the impact that multi-clone, complex infections have on a parasite population structure in a low transmission setting. In general, complexity of infection (minimum number of clones within an infection) and the overall population level diversity is expected to be minimal in low transmission settings. Additionally, the parasite population structure is predicted to be clonal, rather than sexual due to infrequent parasite inoculation and lack of recombination between genetically distinct clones. However, in this low transmission of the Peruvian Amazon, complex infections are becoming more frequent, in spite of decreasing infection prevalence. In this study, it was hypothesized that sexual recombination between distinct clonal lineages of Plasmodium falciparum parasites were altering the subpopulation structure and effectively maintaining the population-level diversity. Methods Fourteen microsatellite markers were chosen to describe the genetic diversity in 313 naturally occurring P. falciparum infections from Peruvian Amazon. The population and subpopulation structure was characterized by measuring: clusteredness, expected heterozygosity (H e ), allelic richness, private allelic richness, and linkage disequilibrium. Next, microsatellite haplotypes and alleles were correlated with P. falciparum merozoite surface protein 1 Block 2 ( Pf msp1-B2) to examine the presence of recombinant microsatellite haplotypes. Results The parasite population structure consists of six genetically diverse subpopulations of clones, called "clusters". Clusters 1, 3, 4, and 6 have unique haplotypes that exceed 70% of the total number of clones within each cluster, while Clusters 2 and 5 have a lower proportion of unique haplotypes, but still exceed 46%. By measuring the H e , allelic richness, and private allelic richness within each of the six subpopulations, relatively low levels of genetic diversity within each subpopulation (except Cluster 4) are observed. This indicated that the ... |
format |
Article in Journal/Newspaper |
author |
Sutton Patrick L Torres Lindsay P Branch OraLee H |
author_facet |
Sutton Patrick L Torres Lindsay P Branch OraLee H |
author_sort |
Sutton Patrick L |
title |
Sexual recombination is a signature of a persisting malaria epidemic in Peru |
title_short |
Sexual recombination is a signature of a persisting malaria epidemic in Peru |
title_full |
Sexual recombination is a signature of a persisting malaria epidemic in Peru |
title_fullStr |
Sexual recombination is a signature of a persisting malaria epidemic in Peru |
title_full_unstemmed |
Sexual recombination is a signature of a persisting malaria epidemic in Peru |
title_sort |
sexual recombination is a signature of a persisting malaria epidemic in peru |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-329 https://doaj.org/article/690ac946148340c8a17f502bed232578 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 329 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/329 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-329 1475-2875 https://doaj.org/article/690ac946148340c8a17f502bed232578 |
op_doi |
https://doi.org/10.1186/1475-2875-10-329 |
container_title |
Malaria Journal |
container_volume |
10 |
container_issue |
1 |
container_start_page |
329 |
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1766344918147530752 |