Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas
The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whol...
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ftdoajarticles:oai:doaj.org/article:69057feb7beb4da390b830d4f8210b14 2023-05-15T15:58:14+02:00 Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas Shanshan Yao Li Li Xudong Guan Yan He Aude Jouaux Fei Xu Ximing Guo Guofan Zhang Linlin Zhang 2022-08-01T00:00:00Z https://doi.org/10.3389/fimmu.2022.928628 https://doaj.org/article/69057feb7beb4da390b830d4f8210b14 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2022.928628/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2022.928628 https://doaj.org/article/69057feb7beb4da390b830d4f8210b14 Frontiers in Immunology, Vol 13 (2022) Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance Immunologic diseases. Allergy RC581-607 article 2022 ftdoajarticles https://doi.org/10.3389/fimmu.2022.928628 2022-12-30T20:44:45Z The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Immunology 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance Immunologic diseases. Allergy RC581-607 Shanshan Yao Li Li Xudong Guan Yan He Aude Jouaux Fei Xu Ximing Guo Guofan Zhang Linlin Zhang Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
topic_facet |
Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance Immunologic diseases. Allergy RC581-607 |
description |
The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance. |
format |
Article in Journal/Newspaper |
author |
Shanshan Yao Li Li Xudong Guan Yan He Aude Jouaux Fei Xu Ximing Guo Guofan Zhang Linlin Zhang |
author_facet |
Shanshan Yao Li Li Xudong Guan Yan He Aude Jouaux Fei Xu Ximing Guo Guofan Zhang Linlin Zhang |
author_sort |
Shanshan Yao |
title |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_short |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_full |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_fullStr |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_sort |
pooled resequencing of larvae and adults reveals genomic variations associated with ostreid herpesvirus 1 resistance in the pacific oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fimmu.2022.928628 https://doaj.org/article/69057feb7beb4da390b830d4f8210b14 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2022.928628/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2022.928628 https://doaj.org/article/69057feb7beb4da390b830d4f8210b14 |
op_doi |
https://doi.org/10.3389/fimmu.2022.928628 |
container_title |
Frontiers in Immunology |
container_volume |
13 |
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1766393967749890048 |