Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968
Objective: To compare the in vitro antioxidant capacity of a diatom, Chaetoceros calcitrans (C. calcitrans) extracted using six types of solvents. Methods: Each extract was evaluated in terms of extraction yield, total carotenoid, fucoxanthin content, total phenolic and antioxidant capacities (DPPH·...
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Wolters Kluwer Medknow Publications
2015
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ftdoajarticles:oai:doaj.org/article:68e68f35981d44e8ac2ed7891f00aade 2023-05-15T15:06:16+02:00 Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 Su Chern Foo Fatimah Md. Yusoff Maznah Ismail Mahiran Basri Nicholas Mun Hoe Khong Kim Wei Chan Sook Kun Yau 2015-10-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.06.003 https://doaj.org/article/68e68f35981d44e8ac2ed7891f00aade EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115001306 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.06.003 https://doaj.org/article/68e68f35981d44e8ac2ed7891f00aade Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 10, Pp 834-840 (2015) Chaetoceros calcitrans Microalgae Diatoms Antioxidants Fucoxanthin Phenolic acids Efficient solvent extraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.06.003 2022-12-31T12:15:54Z Objective: To compare the in vitro antioxidant capacity of a diatom, Chaetoceros calcitrans (C. calcitrans) extracted using six types of solvents. Methods: Each extract was evaluated in terms of extraction yield, total carotenoid, fucoxanthin content, total phenolic and antioxidant capacities (DPPH·− and ABTS·−+ scavenging activity and iron chelating activity). Results: The methanol extract exhibited the highest yield [(22.71 ± 0.96) g/100 g dry weight (DW)], total carotenoid [(4.46 ± 0.36) mg/g DW], total phenolic [(2.49 ± 0.08) mg gallic acid equivalents/g DW] and second highest fucoxanthin content [(2.08 ± 0.03) mg fucoxanthin/g DW] as compared to other solvent extracts. Methanolic extract also exhibited significantly higher (P < 0.05) scavenging (DPPH·−, ABTS·−+) and iron chelating activities. Conclusions: Methanol was the recommended solvent for the production of antioxidant rich extract from C. calcitrans. Both carotenoids and phenolic acids were found to be positively correlated to the antioxidant capacities of C. calcitrans. Lead bioactives confirmed by subsequent high performance liquid chromatography studies were fucoxanthin, gallic acid and protocatechuic acid. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 10 834 840 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Chaetoceros calcitrans Microalgae Diatoms Antioxidants Fucoxanthin Phenolic acids Efficient solvent extraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Chaetoceros calcitrans Microalgae Diatoms Antioxidants Fucoxanthin Phenolic acids Efficient solvent extraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Su Chern Foo Fatimah Md. Yusoff Maznah Ismail Mahiran Basri Nicholas Mun Hoe Khong Kim Wei Chan Sook Kun Yau Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
topic_facet |
Chaetoceros calcitrans Microalgae Diatoms Antioxidants Fucoxanthin Phenolic acids Efficient solvent extraction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To compare the in vitro antioxidant capacity of a diatom, Chaetoceros calcitrans (C. calcitrans) extracted using six types of solvents. Methods: Each extract was evaluated in terms of extraction yield, total carotenoid, fucoxanthin content, total phenolic and antioxidant capacities (DPPH·− and ABTS·−+ scavenging activity and iron chelating activity). Results: The methanol extract exhibited the highest yield [(22.71 ± 0.96) g/100 g dry weight (DW)], total carotenoid [(4.46 ± 0.36) mg/g DW], total phenolic [(2.49 ± 0.08) mg gallic acid equivalents/g DW] and second highest fucoxanthin content [(2.08 ± 0.03) mg fucoxanthin/g DW] as compared to other solvent extracts. Methanolic extract also exhibited significantly higher (P < 0.05) scavenging (DPPH·−, ABTS·−+) and iron chelating activities. Conclusions: Methanol was the recommended solvent for the production of antioxidant rich extract from C. calcitrans. Both carotenoids and phenolic acids were found to be positively correlated to the antioxidant capacities of C. calcitrans. Lead bioactives confirmed by subsequent high performance liquid chromatography studies were fucoxanthin, gallic acid and protocatechuic acid. |
format |
Article in Journal/Newspaper |
author |
Su Chern Foo Fatimah Md. Yusoff Maznah Ismail Mahiran Basri Nicholas Mun Hoe Khong Kim Wei Chan Sook Kun Yau |
author_facet |
Su Chern Foo Fatimah Md. Yusoff Maznah Ismail Mahiran Basri Nicholas Mun Hoe Khong Kim Wei Chan Sook Kun Yau |
author_sort |
Su Chern Foo |
title |
Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
title_short |
Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
title_full |
Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
title_fullStr |
Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
title_full_unstemmed |
Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
title_sort |
efficient solvent extraction of antioxidant-rich extract from a tropical diatom, chaetoceros calcitrans (paulsen) takano 1968 |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.06.003 https://doaj.org/article/68e68f35981d44e8ac2ed7891f00aade |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 10, Pp 834-840 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115001306 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.06.003 https://doaj.org/article/68e68f35981d44e8ac2ed7891f00aade |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.06.003 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
10 |
container_start_page |
834 |
op_container_end_page |
840 |
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1766337905109762048 |