Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world...
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ftdoajarticles:oai:doaj.org/article:68c80c23a5744764895c92cda4742352 2023-05-15T15:13:17+02:00 Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009442 https://doaj.org/article/68c80c23a5744764895c92cda4742352 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009442 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009442 https://doaj.org/article/68c80c23a5744764895c92cda4742352 PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009442 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009442 2022-12-31T03:56:27Z Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world's population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector. Article in Journal/Newspaper Arctic Climate change Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009442 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world's population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector. |
format |
Article in Journal/Newspaper |
author |
Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig |
author_facet |
Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig |
author_sort |
Alejandro Marin-Lopez |
title |
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. |
title_short |
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. |
title_full |
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. |
title_fullStr |
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. |
title_full_unstemmed |
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination. |
title_sort |
aedes aegypti snap and a calcium transporter atpase influence dengue virus dissemination. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009442 https://doaj.org/article/68c80c23a5744764895c92cda4742352 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change |
genre_facet |
Arctic Climate change |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009442 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009442 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009442 https://doaj.org/article/68c80c23a5744764895c92cda4742352 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009442 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
6 |
container_start_page |
e0009442 |
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1766343850034462720 |