Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species
As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat toler...
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ftdoajarticles:oai:doaj.org/article:68c5699ea47a4d9dbcdcf734d77cfd4d 2024-02-11T10:03:08+01:00 Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species Mingyang Du Zhuxiang Jiang Chaogang Wang Chenchen Wei Qingyuan Li Rihao Cong Wei Wang Guofan Zhang Li Li 2023-12-01T00:00:00Z https://doi.org/10.3390/ijms25010125 https://doaj.org/article/68c5699ea47a4d9dbcdcf734d77cfd4d EN eng MDPI AG https://www.mdpi.com/1422-0067/25/1/125 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms25010125 1422-0067 1661-6596 https://doaj.org/article/68c5699ea47a4d9dbcdcf734d77cfd4d International Journal of Molecular Sciences, Vol 25, Iss 1, p 125 (2023) oysters heat tolerance GWAS Crassostrea gigas Crassostrea angulata F 2 progeny Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms25010125 2024-01-14T01:39:06Z As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F 2 generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata ♀ and heat-sensitive Crassostrea gigas ♂, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 °C) demonstrated that the F 2 population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor ( Trpm2 ), transcriptional factor ( Gata3 ), protein ubiquitination ( Ube2h , Usp50 , Uchl3 ), heat shock subfamily ( Dnajc17 , Dnaja1 ), and transporters ( Slc16a9 , Slc16a14 , Slc16a9 , Slc16a2 ). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 °C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 25 1 125 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
oysters heat tolerance GWAS Crassostrea gigas Crassostrea angulata F 2 progeny Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
oysters heat tolerance GWAS Crassostrea gigas Crassostrea angulata F 2 progeny Biology (General) QH301-705.5 Chemistry QD1-999 Mingyang Du Zhuxiang Jiang Chaogang Wang Chenchen Wei Qingyuan Li Rihao Cong Wei Wang Guofan Zhang Li Li Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species |
topic_facet |
oysters heat tolerance GWAS Crassostrea gigas Crassostrea angulata F 2 progeny Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F 2 generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata ♀ and heat-sensitive Crassostrea gigas ♂, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 °C) demonstrated that the F 2 population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor ( Trpm2 ), transcriptional factor ( Gata3 ), protein ubiquitination ( Ube2h , Usp50 , Uchl3 ), heat shock subfamily ( Dnajc17 , Dnaja1 ), and transporters ( Slc16a9 , Slc16a14 , Slc16a9 , Slc16a2 ). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 °C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry. |
format |
Article in Journal/Newspaper |
author |
Mingyang Du Zhuxiang Jiang Chaogang Wang Chenchen Wei Qingyuan Li Rihao Cong Wei Wang Guofan Zhang Li Li |
author_facet |
Mingyang Du Zhuxiang Jiang Chaogang Wang Chenchen Wei Qingyuan Li Rihao Cong Wei Wang Guofan Zhang Li Li |
author_sort |
Mingyang Du |
title |
Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species |
title_short |
Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species |
title_full |
Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species |
title_fullStr |
Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species |
title_full_unstemmed |
Genome-Wide Association Analysis of Heat Tolerance in F 2 Progeny from the Hybridization between Two Congeneric Oyster Species |
title_sort |
genome-wide association analysis of heat tolerance in f 2 progeny from the hybridization between two congeneric oyster species |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms25010125 https://doaj.org/article/68c5699ea47a4d9dbcdcf734d77cfd4d |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
International Journal of Molecular Sciences, Vol 25, Iss 1, p 125 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/25/1/125 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms25010125 1422-0067 1661-6596 https://doaj.org/article/68c5699ea47a4d9dbcdcf734d77cfd4d |
op_doi |
https://doi.org/10.3390/ijms25010125 |
container_title |
International Journal of Molecular Sciences |
container_volume |
25 |
container_issue |
1 |
container_start_page |
125 |
_version_ |
1790599306639572992 |