Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison
Abstract Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPas...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-1484-18 https://doaj.org/article/689ae6f337e04c508725eaad7dd38893 |
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ftdoajarticles:oai:doaj.org/article:689ae6f337e04c508725eaad7dd38893 2023-05-15T15:17:24+02:00 Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison Mateus Amaral Baldo Alexandra Olimpio Siqueira Cunha Lívea Dornela Godoy José Luiz Liberato Juliana Sakamoto Yoneda Elisa Correa Fornari-Baldo Pietro Ciancaglini Wagner Ferreira dos Santos Eliane Candiani Arantes 2019-04-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-1484-18 https://doaj.org/article/689ae6f337e04c508725eaad7dd38893 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100309&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-1484-18 https://doaj.org/article/689ae6f337e04c508725eaad7dd38893 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25, Iss 0 (2019) Rhinella schneideri toad poison bufadienolides seizures neuroprotection Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2019 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-1484-18 2022-12-31T00:37:01Z Abstract Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with actions of biotechnological interest. Methods: Rhinella schneideri toad (male and female) poison was collected by pressuring their parotoid glands and immediately dried and stored at -20 °C. The poison was dialysed and the water containing the low molecular mass molecules (< 8 kDa) that permeate the dialysis membrane was collected, frozen and lyophilized, resulting in the sample used in the assays, named low molecular weight fraction (LMWF). Na+/K+ ATPase was isolated from rabbit kidneys and enzyme activity assays performed by the quantification of phosphate released due to enzyme activity in the presence of LMWF (1.0; 10; 50 and 100 µg/mL) from Rhinella schneideri poison. Evaluation of the L-Glutamate (L-Glu) excitatory amino acid uptake in brain-cortical synaptosomes of Wistar rats was performed using [3H]L-glutamate and different concentration of LMWF (10-5 to 10 µg/µL). Anticonvulsant assays were performed using pentylenetetrazole (PTZ) and N-methyl-D-aspartate (NMDA) to induce seizures in Wistar rats (n= 6), which were cannulated in the lateral ventricle and treated with different concentration of LMWF (0.25; 0.5; 1.0; 2.0; 3.0 and 4.0 µg/µL) 15 min prior to the injection of the seizure agent. Results: LMWF induced a concentration-dependent inhibition of Na+/K+-ATPase (IC50% = 107.5 μg/mL). The poison induces an increased uptake of the amino acid L-glutamate in brain-cortical synaptosomes ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 25 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Rhinella schneideri toad poison bufadienolides seizures neuroprotection Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Rhinella schneideri toad poison bufadienolides seizures neuroprotection Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Mateus Amaral Baldo Alexandra Olimpio Siqueira Cunha Lívea Dornela Godoy José Luiz Liberato Juliana Sakamoto Yoneda Elisa Correa Fornari-Baldo Pietro Ciancaglini Wagner Ferreira dos Santos Eliane Candiani Arantes Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison |
topic_facet |
Rhinella schneideri toad poison bufadienolides seizures neuroprotection Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with actions of biotechnological interest. Methods: Rhinella schneideri toad (male and female) poison was collected by pressuring their parotoid glands and immediately dried and stored at -20 °C. The poison was dialysed and the water containing the low molecular mass molecules (< 8 kDa) that permeate the dialysis membrane was collected, frozen and lyophilized, resulting in the sample used in the assays, named low molecular weight fraction (LMWF). Na+/K+ ATPase was isolated from rabbit kidneys and enzyme activity assays performed by the quantification of phosphate released due to enzyme activity in the presence of LMWF (1.0; 10; 50 and 100 µg/mL) from Rhinella schneideri poison. Evaluation of the L-Glutamate (L-Glu) excitatory amino acid uptake in brain-cortical synaptosomes of Wistar rats was performed using [3H]L-glutamate and different concentration of LMWF (10-5 to 10 µg/µL). Anticonvulsant assays were performed using pentylenetetrazole (PTZ) and N-methyl-D-aspartate (NMDA) to induce seizures in Wistar rats (n= 6), which were cannulated in the lateral ventricle and treated with different concentration of LMWF (0.25; 0.5; 1.0; 2.0; 3.0 and 4.0 µg/µL) 15 min prior to the injection of the seizure agent. Results: LMWF induced a concentration-dependent inhibition of Na+/K+-ATPase (IC50% = 107.5 μg/mL). The poison induces an increased uptake of the amino acid L-glutamate in brain-cortical synaptosomes ... |
format |
Article in Journal/Newspaper |
author |
Mateus Amaral Baldo Alexandra Olimpio Siqueira Cunha Lívea Dornela Godoy José Luiz Liberato Juliana Sakamoto Yoneda Elisa Correa Fornari-Baldo Pietro Ciancaglini Wagner Ferreira dos Santos Eliane Candiani Arantes |
author_facet |
Mateus Amaral Baldo Alexandra Olimpio Siqueira Cunha Lívea Dornela Godoy José Luiz Liberato Juliana Sakamoto Yoneda Elisa Correa Fornari-Baldo Pietro Ciancaglini Wagner Ferreira dos Santos Eliane Candiani Arantes |
author_sort |
Mateus Amaral Baldo |
title |
Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison |
title_short |
Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison |
title_full |
Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison |
title_fullStr |
Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison |
title_full_unstemmed |
Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison |
title_sort |
assessment of neuropharmacological potential of low molecular weight components extracted from rhinella schneideri toad poison |
publisher |
SciELO |
publishDate |
2019 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-1484-18 https://doaj.org/article/689ae6f337e04c508725eaad7dd38893 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25, Iss 0 (2019) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100309&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-1484-18 https://doaj.org/article/689ae6f337e04c508725eaad7dd38893 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-1484-18 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
25 |
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1766347649874657280 |