Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry
Phlorotannins are secondary metabolites produced mainly by brown seaweeds (Phaeophyceae) and belong to the class of polyphenolic compounds with diverse bioactivities. The key factors in the extraction of polyphenols are the selection of a suitable solvent, method of extraction and selection of optim...
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MDPI AG
2023
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ftdoajarticles:oai:doaj.org/article:689a1dd421734a2b8b25883a2026e32c 2023-06-11T04:09:05+02:00 Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry Ekaterina D. Obluchinskaya Olga N. Pozharitskaya Vadim A. Shevyrin Elena G. Kovaleva Elena V. Flisyuk Alexander N. Shikov 2023-04-01T00:00:00Z https://doi.org/10.3390/md21050263 https://doaj.org/article/689a1dd421734a2b8b25883a2026e32c EN eng MDPI AG https://www.mdpi.com/1660-3397/21/5/263 https://doaj.org/toc/1660-3397 doi:10.3390/md21050263 1660-3397 https://doaj.org/article/689a1dd421734a2b8b25883a2026e32c Marine Drugs, Vol 21, Iss 263, p 263 (2023) Arctic alga seaweed Fucus vesiculosus extraction natural deep eutectic solvent Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/md21050263 2023-05-28T00:33:43Z Phlorotannins are secondary metabolites produced mainly by brown seaweeds (Phaeophyceae) and belong to the class of polyphenolic compounds with diverse bioactivities. The key factors in the extraction of polyphenols are the selection of a suitable solvent, method of extraction and selection of optimal conditions. Ultrasonic-assisted extraction (UAE) is one of the advanced energy-saving methods suitable for the extraction of labile compounds. Methanol, acetone, ethanol and ethyl acetate are the most commonly used solvents for polyphenol extraction. As alternatives to toxic organic solvents, a new class of green solvents, natural deep eutectic solvents (NADES), has been proposed for the efficient extraction of a wide range of natural compounds including polyphenols. Several NADES were screened previously for the extraction of phlorotannins; however, the extraction conditions were not optimized and chemical profiling of NADES extract was not performed. The purpose of this work was to study the effect of selected extraction parameters on the phlorotannin content in NADES extract from Fucus vesiculosus, optimization of extraction conditions and chemical profiling of phlorotannins in the NADES extract. A fast and green NADES-UAE procedure was developed for the extraction of phlorotannins. Optimization was performed through an experimental design and showed that NADES (lactic acid:choline chloride; 3:1) provides a high yield (137.3 mg phloroglucinol equivalents per g dry weight of algae) of phlorotannins under the following extraction conditions: extraction time 23 min, 30.0% water concentration and 1:12 sample to solvent ratio. The antioxidant activity of the optimized NADES extract was equal to that of EtOH extract. In total, 32 phlorotannins have been identified (one trimer, two tetramers, six pentamers, four hexamers, six heptamers, six octamers and seven nonamers) in NADES extracts from arctic F. vesiculosus using the HPLC-HRMS and MS/MS technique. It was noted that all the above-mentioned phlorotannins were ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Marine Drugs 21 5 263 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic alga seaweed Fucus vesiculosus extraction natural deep eutectic solvent Biology (General) QH301-705.5 |
spellingShingle |
Arctic alga seaweed Fucus vesiculosus extraction natural deep eutectic solvent Biology (General) QH301-705.5 Ekaterina D. Obluchinskaya Olga N. Pozharitskaya Vadim A. Shevyrin Elena G. Kovaleva Elena V. Flisyuk Alexander N. Shikov Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry |
topic_facet |
Arctic alga seaweed Fucus vesiculosus extraction natural deep eutectic solvent Biology (General) QH301-705.5 |
description |
Phlorotannins are secondary metabolites produced mainly by brown seaweeds (Phaeophyceae) and belong to the class of polyphenolic compounds with diverse bioactivities. The key factors in the extraction of polyphenols are the selection of a suitable solvent, method of extraction and selection of optimal conditions. Ultrasonic-assisted extraction (UAE) is one of the advanced energy-saving methods suitable for the extraction of labile compounds. Methanol, acetone, ethanol and ethyl acetate are the most commonly used solvents for polyphenol extraction. As alternatives to toxic organic solvents, a new class of green solvents, natural deep eutectic solvents (NADES), has been proposed for the efficient extraction of a wide range of natural compounds including polyphenols. Several NADES were screened previously for the extraction of phlorotannins; however, the extraction conditions were not optimized and chemical profiling of NADES extract was not performed. The purpose of this work was to study the effect of selected extraction parameters on the phlorotannin content in NADES extract from Fucus vesiculosus, optimization of extraction conditions and chemical profiling of phlorotannins in the NADES extract. A fast and green NADES-UAE procedure was developed for the extraction of phlorotannins. Optimization was performed through an experimental design and showed that NADES (lactic acid:choline chloride; 3:1) provides a high yield (137.3 mg phloroglucinol equivalents per g dry weight of algae) of phlorotannins under the following extraction conditions: extraction time 23 min, 30.0% water concentration and 1:12 sample to solvent ratio. The antioxidant activity of the optimized NADES extract was equal to that of EtOH extract. In total, 32 phlorotannins have been identified (one trimer, two tetramers, six pentamers, four hexamers, six heptamers, six octamers and seven nonamers) in NADES extracts from arctic F. vesiculosus using the HPLC-HRMS and MS/MS technique. It was noted that all the above-mentioned phlorotannins were ... |
format |
Article in Journal/Newspaper |
author |
Ekaterina D. Obluchinskaya Olga N. Pozharitskaya Vadim A. Shevyrin Elena G. Kovaleva Elena V. Flisyuk Alexander N. Shikov |
author_facet |
Ekaterina D. Obluchinskaya Olga N. Pozharitskaya Vadim A. Shevyrin Elena G. Kovaleva Elena V. Flisyuk Alexander N. Shikov |
author_sort |
Ekaterina D. Obluchinskaya |
title |
Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry |
title_short |
Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry |
title_full |
Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry |
title_fullStr |
Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry |
title_full_unstemmed |
Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry |
title_sort |
optimization of extraction of phlorotannins from the arctic fucus vesiculosus using natural deep eutectic solvents and their hplc profiling with tandem high-resolution mass spectrometry |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/md21050263 https://doaj.org/article/689a1dd421734a2b8b25883a2026e32c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Marine Drugs, Vol 21, Iss 263, p 263 (2023) |
op_relation |
https://www.mdpi.com/1660-3397/21/5/263 https://doaj.org/toc/1660-3397 doi:10.3390/md21050263 1660-3397 https://doaj.org/article/689a1dd421734a2b8b25883a2026e32c |
op_doi |
https://doi.org/10.3390/md21050263 |
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Marine Drugs |
container_volume |
21 |
container_issue |
5 |
container_start_page |
263 |
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1768382793152200704 |