Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia
Introduction: Fusarium is a very heterogeneous group of fungi, difficult to classify, with a wide range of living styles, acting as saprophytes, parasites of plants, or pathogens for humans and animals. Prevalence of clinical fusariosis and lack of effective treatments have increased the interest in...
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ftdoajarticles:oai:doaj.org/article:68627c04887f404295039d232706136c 2023-05-15T15:09:46+02:00 Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia Valeria Velásquez-Zapata Katherine Palacio-Rúa Luz E. Cano Adelaida Gaviria-Rivera 2022-03-01T00:00:00Z https://doi.org/10.7705/biomedica.5869 https://doaj.org/article/68627c04887f404295039d232706136c EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5869 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5869 https://doaj.org/article/68627c04887f404295039d232706136c Biomédica: revista del Instituto Nacional de Salud, Vol 42, Iss 1, Pp 18-30 (2022) fusarium fusariosis genotyping techniques bacteriophage m13 elongin genetics population dna fingerprinting Medicine R Arctic medicine. Tropical medicine RC955-962 article 2022 ftdoajarticles https://doi.org/10.7705/biomedica.5869 2022-12-30T23:56:45Z Introduction: Fusarium is a very heterogeneous group of fungi, difficult to classify, with a wide range of living styles, acting as saprophytes, parasites of plants, or pathogens for humans and animals. Prevalence of clinical fusariosis and lack of effective treatments have increased the interest in the precise diagnosis, which implies a molecular characterization of Fusarium populations. Objective: We compared different genotyping markers in their assessment of the genetic variability and molecular identification of clinical isolates of Fusarium. Materials and methods: We evaluated the performance of the fingerprinting produced by two random primers: M13, which amplifies a minisatellite sequence, and (GACA)4, which corresponds to a simple repetitive DNA sequence. Using the Hunter Gaston Discriminatory Index (HGDI), an analysis of molecular variance (AMOVA), and a Mantel test, the resolution of these markers was compared to the reference sequencing-based and PCR genotyping methods. Results: The highest HGDI value was associated with the M13 marker followed by (GACA)4. AMOVA and the Mantel tests supported a strong correlation between the M13 classification and the reference method given by the partial sequencing of the transcription elongation factor 1-alpha (TEF1-α) and rDNA 28S. Conclusion: The strong correlation between the M13 classification and the sequencingbased reference together with its higher resolution demonstrates its adequacy for the characterization of Fusarium populations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Gaston ENVELOPE(65.783,65.783,-70.417,-70.417) Biomédica 42 1 18 30 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
fusarium fusariosis genotyping techniques bacteriophage m13 elongin genetics population dna fingerprinting Medicine R Arctic medicine. Tropical medicine RC955-962 |
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fusarium fusariosis genotyping techniques bacteriophage m13 elongin genetics population dna fingerprinting Medicine R Arctic medicine. Tropical medicine RC955-962 Valeria Velásquez-Zapata Katherine Palacio-Rúa Luz E. Cano Adelaida Gaviria-Rivera Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia |
topic_facet |
fusarium fusariosis genotyping techniques bacteriophage m13 elongin genetics population dna fingerprinting Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Fusarium is a very heterogeneous group of fungi, difficult to classify, with a wide range of living styles, acting as saprophytes, parasites of plants, or pathogens for humans and animals. Prevalence of clinical fusariosis and lack of effective treatments have increased the interest in the precise diagnosis, which implies a molecular characterization of Fusarium populations. Objective: We compared different genotyping markers in their assessment of the genetic variability and molecular identification of clinical isolates of Fusarium. Materials and methods: We evaluated the performance of the fingerprinting produced by two random primers: M13, which amplifies a minisatellite sequence, and (GACA)4, which corresponds to a simple repetitive DNA sequence. Using the Hunter Gaston Discriminatory Index (HGDI), an analysis of molecular variance (AMOVA), and a Mantel test, the resolution of these markers was compared to the reference sequencing-based and PCR genotyping methods. Results: The highest HGDI value was associated with the M13 marker followed by (GACA)4. AMOVA and the Mantel tests supported a strong correlation between the M13 classification and the reference method given by the partial sequencing of the transcription elongation factor 1-alpha (TEF1-α) and rDNA 28S. Conclusion: The strong correlation between the M13 classification and the sequencingbased reference together with its higher resolution demonstrates its adequacy for the characterization of Fusarium populations. |
format |
Article in Journal/Newspaper |
author |
Valeria Velásquez-Zapata Katherine Palacio-Rúa Luz E. Cano Adelaida Gaviria-Rivera |
author_facet |
Valeria Velásquez-Zapata Katherine Palacio-Rúa Luz E. Cano Adelaida Gaviria-Rivera |
author_sort |
Valeria Velásquez-Zapata |
title |
Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia |
title_short |
Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia |
title_full |
Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia |
title_fullStr |
Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia |
title_full_unstemmed |
Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia |
title_sort |
assessment of genotyping markers in the molecular characterization of a population of clinical isolates of fusarium in colombia |
publisher |
Instituto Nacional de Salud |
publishDate |
2022 |
url |
https://doi.org/10.7705/biomedica.5869 https://doaj.org/article/68627c04887f404295039d232706136c |
long_lat |
ENVELOPE(65.783,65.783,-70.417,-70.417) |
geographic |
Arctic Gaston |
geographic_facet |
Arctic Gaston |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 42, Iss 1, Pp 18-30 (2022) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/5869 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5869 https://doaj.org/article/68627c04887f404295039d232706136c |
op_doi |
https://doi.org/10.7705/biomedica.5869 |
container_title |
Biomédica |
container_volume |
42 |
container_issue |
1 |
container_start_page |
18 |
op_container_end_page |
30 |
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1766340896250396672 |