Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica
The aim of this research was to evaluate the prevalence of Sthaphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of beta-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:685a03e7cd584945a2d045c553f1d465 2024-09-09T19:27:49+00:00 Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica Wagner Luis de Carvalho Bernardo Marcelo Fabiano Gomes Boriollo Reginaldo Bruno Gonçalves José Francisco Höfling 2005-02-01T00:00:00Z https://doi.org/10.1590/S0036-46652005000100004 https://doaj.org/article/685a03e7cd584945a2d045c553f1d465 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000100004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652005000100004 0036-4665 1678-9946 https://doaj.org/article/685a03e7cd584945a2d045c553f1d465 Revista do Instituto de Medicina Tropical de São Paulo, Vol 47, Iss 1, Pp 19-24 (2005) Staphylococcus aureus Ampicillin-resistant Odontological clinic environment Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2005 ftdoajarticles https://doi.org/10.1590/S0036-46652005000100004 2024-08-05T17:49:30Z The aim of this research was to evaluate the prevalence of Sthaphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of beta-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect were isolated 9775 CFU by MSA medium suggesting a high amount of Staphylococcus spp. in the clinic environment which can appear through aerosols. A total of 3149 colonies (32.2%) were suggestive of pathogenic staphylococci. Gram coloration, catalase test, colony-mallow growing on chromogenic medium, and coagulase test confirmed the identity of 44 (0.45%) S. aureus isolates. Of these, 35 isolates (79.5%) showed production of beta-lactamase by CefinaseTM discs and resistance to ampicillin, erythromycin (7 isolates) and tetracycline (1 isolate) suggesting the existence of multiresistant isolates. The evaluation of the oxacillin MIC by Etest® assays showed susceptibility patterns suggesting the inexistence of the mecA gene in chromosomal DNA. These results point out to the need of a larger knowledge on the contamination means and propagation of this microorganism into the odontological clinic. Foi avaliada a prevalência de Staphylococcus spp. e S. aureus no ambiente clínico odontológico, a produção de beta-lactamase e a susceptibilidade antibacteriana aos principais antibióticos utilizados na prática clínica. Durante 12 meses de coleta de amostras foram isolados 9775 UFC no meio de cultura AMS, demonstrando uma elevada quantidade de Staphylococcus spp. no ambiente clínico, provavelmente em decorrência da propagação de aerossóis. Um total de 3149 colônias (32,2%) foi sugestivo de estafilococos patogênicos. Coloração de Gram, teste de catalase, crescimento de colônias-malva sobre meio cromogênico e teste de coagulase confirmaram a identidade de 44 (0,45%) isolados de S. aureus. Destes, 35 isolados (79,5%) mostraram produção de beta-lactamase através de discos de CefinaseTM e resistência a ampicilina, eritromicina (7 ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 47 1 19 24 |
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language |
English |
topic |
Staphylococcus aureus Ampicillin-resistant Odontological clinic environment Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Staphylococcus aureus Ampicillin-resistant Odontological clinic environment Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Wagner Luis de Carvalho Bernardo Marcelo Fabiano Gomes Boriollo Reginaldo Bruno Gonçalves José Francisco Höfling Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica |
topic_facet |
Staphylococcus aureus Ampicillin-resistant Odontological clinic environment Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The aim of this research was to evaluate the prevalence of Sthaphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of beta-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect were isolated 9775 CFU by MSA medium suggesting a high amount of Staphylococcus spp. in the clinic environment which can appear through aerosols. A total of 3149 colonies (32.2%) were suggestive of pathogenic staphylococci. Gram coloration, catalase test, colony-mallow growing on chromogenic medium, and coagulase test confirmed the identity of 44 (0.45%) S. aureus isolates. Of these, 35 isolates (79.5%) showed production of beta-lactamase by CefinaseTM discs and resistance to ampicillin, erythromycin (7 isolates) and tetracycline (1 isolate) suggesting the existence of multiresistant isolates. The evaluation of the oxacillin MIC by Etest® assays showed susceptibility patterns suggesting the inexistence of the mecA gene in chromosomal DNA. These results point out to the need of a larger knowledge on the contamination means and propagation of this microorganism into the odontological clinic. Foi avaliada a prevalência de Staphylococcus spp. e S. aureus no ambiente clínico odontológico, a produção de beta-lactamase e a susceptibilidade antibacteriana aos principais antibióticos utilizados na prática clínica. Durante 12 meses de coleta de amostras foram isolados 9775 UFC no meio de cultura AMS, demonstrando uma elevada quantidade de Staphylococcus spp. no ambiente clínico, provavelmente em decorrência da propagação de aerossóis. Um total de 3149 colônias (32,2%) foi sugestivo de estafilococos patogênicos. Coloração de Gram, teste de catalase, crescimento de colônias-malva sobre meio cromogênico e teste de coagulase confirmaram a identidade de 44 (0,45%) isolados de S. aureus. Destes, 35 isolados (79,5%) mostraram produção de beta-lactamase através de discos de CefinaseTM e resistência a ampicilina, eritromicina (7 ... |
format |
Article in Journal/Newspaper |
author |
Wagner Luis de Carvalho Bernardo Marcelo Fabiano Gomes Boriollo Reginaldo Bruno Gonçalves José Francisco Höfling |
author_facet |
Wagner Luis de Carvalho Bernardo Marcelo Fabiano Gomes Boriollo Reginaldo Bruno Gonçalves José Francisco Höfling |
author_sort |
Wagner Luis de Carvalho Bernardo |
title |
Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica |
title_short |
Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica |
title_full |
Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica |
title_fullStr |
Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica |
title_full_unstemmed |
Staphylococcus aureus ampicillin-resistant from the odontological clinic environment Staphylococcus aureus resistente à ampicilina em ambiente de Clínica Odontológica |
title_sort |
staphylococcus aureus ampicillin-resistant from the odontological clinic environment staphylococcus aureus resistente à ampicilina em ambiente de clínica odontológica |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2005 |
url |
https://doi.org/10.1590/S0036-46652005000100004 https://doaj.org/article/685a03e7cd584945a2d045c553f1d465 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 47, Iss 1, Pp 19-24 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000100004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652005000100004 0036-4665 1678-9946 https://doaj.org/article/685a03e7cd584945a2d045c553f1d465 |
op_doi |
https://doi.org/10.1590/S0036-46652005000100004 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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47 |
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