Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia
Abstract Background The increase in detections of Plasmodium vivax infection in Duffy-negative individuals in Africa has challenged the dogma establishing the unique P. vivax Duffy Binding Protein-Duffy antigen receptor for chemokines (PvDBP-DARC) pathway used by P. vivax merozoites to invade reticu...
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ftdoajarticles:oai:doaj.org/article:6839f10025c4448ba4f93367ef7e3db8 2023-05-15T15:15:49+02:00 Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia Andargie Abate Isabelle Bouyssou Solenne Mabilotte Cecile Doderer-Lang Laurent Dembele Didier Menard Lemu Golassa 2022-08-01T00:00:00Z https://doi.org/10.1186/s12936-022-04250-2 https://doaj.org/article/6839f10025c4448ba4f93367ef7e3db8 EN eng BMC https://doi.org/10.1186/s12936-022-04250-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04250-2 1475-2875 https://doaj.org/article/6839f10025c4448ba4f93367ef7e3db8 Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) Duffy antigen Polymorphism Asexual parasitaemia Ethiopia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04250-2 2022-12-30T23:14:50Z Abstract Background The increase in detections of Plasmodium vivax infection in Duffy-negative individuals in Africa has challenged the dogma establishing the unique P. vivax Duffy Binding Protein-Duffy antigen receptor for chemokines (PvDBP-DARC) pathway used by P. vivax merozoites to invade reticulocytes. Information on the impact of Duffy antigen polymorphisms on the epidemiology of P. vivax malaria remains elusive. The objective of this study was to determine the distribution of asexual parasitaemia of P. vivax according to the Duffy antigen polymorphisms in Ethiopia. Methods DNA was extracted from dried blood spots (DBS) collected from prospectively recruited 138 P. vivax-infected patients from health centres. The identification and estimation of P. vivax asexual parasitaemia were performed by microscopic examination and quantitative real-time polymerase chain reaction (PCR). Duffy genotyping was conducted by DNA sequencing in a total of 138 P.vivax infected samples. Results The proportion of Duffy-negatives (FY*BES/FY*BES) in P. vivax infected patients was 2.9% (4/138). Duffy genotype FY*B/FY*BES (48.6%) was the most common, followed by FY*A/FY*BES genotype (25.4%). In one patient, the FY*02 W.01/FY*02 N.01 genotype conferring a weak expression of the Fyb antigen was observed. All P.vivax infected Duffy-negative patients showed low asexual parasitaemia (≤ 110 parasites/µL). The median P. vivax parasitaemia in Duffy-negative patients (53 parasites/µL) was significantly lower than those found in homozygous and heterozygous individuals (P < 0.0001). Conclusion Plasmodium vivax in Duffy-negative patients shows invariably low asexual parasitaemia. This finding suggests that the pathway used by P. vivax to invade Duffy-negative reticulocytes is much less efficient than that used in Duffy-positives. Moreover, the low asexual parasitaemia observed in Duffy-negative individuals could constitute an ‘undetected silent reservoir', thus likely delaying the elimination of vivax malaria in Ethiopia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Duffy antigen Polymorphism Asexual parasitaemia Ethiopia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Duffy antigen Polymorphism Asexual parasitaemia Ethiopia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Andargie Abate Isabelle Bouyssou Solenne Mabilotte Cecile Doderer-Lang Laurent Dembele Didier Menard Lemu Golassa Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia |
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Duffy antigen Polymorphism Asexual parasitaemia Ethiopia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The increase in detections of Plasmodium vivax infection in Duffy-negative individuals in Africa has challenged the dogma establishing the unique P. vivax Duffy Binding Protein-Duffy antigen receptor for chemokines (PvDBP-DARC) pathway used by P. vivax merozoites to invade reticulocytes. Information on the impact of Duffy antigen polymorphisms on the epidemiology of P. vivax malaria remains elusive. The objective of this study was to determine the distribution of asexual parasitaemia of P. vivax according to the Duffy antigen polymorphisms in Ethiopia. Methods DNA was extracted from dried blood spots (DBS) collected from prospectively recruited 138 P. vivax-infected patients from health centres. The identification and estimation of P. vivax asexual parasitaemia were performed by microscopic examination and quantitative real-time polymerase chain reaction (PCR). Duffy genotyping was conducted by DNA sequencing in a total of 138 P.vivax infected samples. Results The proportion of Duffy-negatives (FY*BES/FY*BES) in P. vivax infected patients was 2.9% (4/138). Duffy genotype FY*B/FY*BES (48.6%) was the most common, followed by FY*A/FY*BES genotype (25.4%). In one patient, the FY*02 W.01/FY*02 N.01 genotype conferring a weak expression of the Fyb antigen was observed. All P.vivax infected Duffy-negative patients showed low asexual parasitaemia (≤ 110 parasites/µL). The median P. vivax parasitaemia in Duffy-negative patients (53 parasites/µL) was significantly lower than those found in homozygous and heterozygous individuals (P < 0.0001). Conclusion Plasmodium vivax in Duffy-negative patients shows invariably low asexual parasitaemia. This finding suggests that the pathway used by P. vivax to invade Duffy-negative reticulocytes is much less efficient than that used in Duffy-positives. Moreover, the low asexual parasitaemia observed in Duffy-negative individuals could constitute an ‘undetected silent reservoir', thus likely delaying the elimination of vivax malaria in Ethiopia. |
format |
Article in Journal/Newspaper |
author |
Andargie Abate Isabelle Bouyssou Solenne Mabilotte Cecile Doderer-Lang Laurent Dembele Didier Menard Lemu Golassa |
author_facet |
Andargie Abate Isabelle Bouyssou Solenne Mabilotte Cecile Doderer-Lang Laurent Dembele Didier Menard Lemu Golassa |
author_sort |
Andargie Abate |
title |
Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia |
title_short |
Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia |
title_full |
Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia |
title_fullStr |
Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia |
title_full_unstemmed |
Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia |
title_sort |
vivax malaria in duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in ethiopia |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04250-2 https://doaj.org/article/6839f10025c4448ba4f93367ef7e3db8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04250-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04250-2 1475-2875 https://doaj.org/article/6839f10025c4448ba4f93367ef7e3db8 |
op_doi |
https://doi.org/10.1186/s12936-022-04250-2 |
container_title |
Malaria Journal |
container_volume |
21 |
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1 |
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1766346157652443136 |