Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation
Abstract Background Percoll gradient centrifugation is often used for synchronization, enrichment, or isolation of a particular stage of Plasmodium falciparum . However, Percoll, a hyperosmotic agent, may have harmful effects on the parasites. Magnetic bead column (MBC) separation has been used as a...
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ftdoajarticles:oai:doaj.org/article:682cfa92bae2420d8bbb9d2de1de1bad 2023-05-15T15:08:45+02:00 Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation Gerena Lucia Spadafora Carmenza Kopydlowski Karen M 2011-04-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-96 https://doaj.org/article/682cfa92bae2420d8bbb9d2de1de1bad EN eng BMC http://www.malariajournal.com/content/10/1/96 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-96 1475-2875 https://doaj.org/article/682cfa92bae2420d8bbb9d2de1de1bad Malaria Journal, Vol 10, Iss 1, p 96 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-96 2022-12-31T01:43:21Z Abstract Background Percoll gradient centrifugation is often used for synchronization, enrichment, or isolation of a particular stage of Plasmodium falciparum . However, Percoll, a hyperosmotic agent, may have harmful effects on the parasites. Magnetic bead column (MBC) separation has been used as an alternative. This is a report of a head-to-head comparison of the in vitro invasive capabilities of parasites isolated by either of the two methods. Methods The P. falciparum laboratory strain isolate 7G8 was grown in vitro using standard procedures and synchronized using 5% sorbitol. On separate days when the schizont parasitaemia was >1%, the culture was split and half was processed by Percoll gradient centrifugation and the other half by magnetic bead column separation. Both processed parasites were placed back in culture and allowed to invade new uninfected erythrocytes. Results In 10 paired assays, the mean efficiency of invasion of 7G8 parasites treated by Percoll gradient centrifugation was 35.8% that of those treated by magnetic bead column separation (95% CI, p = 0.00067) A paired t test with two tails was used for these comparisons. Conclusions In this comparison, magnetic bead column separation of 7G8 schizonts resulted in higher viability and efficiency of invasion than utilizing Percoll gradient centrifugation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Gerena Lucia Spadafora Carmenza Kopydlowski Karen M Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Percoll gradient centrifugation is often used for synchronization, enrichment, or isolation of a particular stage of Plasmodium falciparum . However, Percoll, a hyperosmotic agent, may have harmful effects on the parasites. Magnetic bead column (MBC) separation has been used as an alternative. This is a report of a head-to-head comparison of the in vitro invasive capabilities of parasites isolated by either of the two methods. Methods The P. falciparum laboratory strain isolate 7G8 was grown in vitro using standard procedures and synchronized using 5% sorbitol. On separate days when the schizont parasitaemia was >1%, the culture was split and half was processed by Percoll gradient centrifugation and the other half by magnetic bead column separation. Both processed parasites were placed back in culture and allowed to invade new uninfected erythrocytes. Results In 10 paired assays, the mean efficiency of invasion of 7G8 parasites treated by Percoll gradient centrifugation was 35.8% that of those treated by magnetic bead column separation (95% CI, p = 0.00067) A paired t test with two tails was used for these comparisons. Conclusions In this comparison, magnetic bead column separation of 7G8 schizonts resulted in higher viability and efficiency of invasion than utilizing Percoll gradient centrifugation. |
format |
Article in Journal/Newspaper |
author |
Gerena Lucia Spadafora Carmenza Kopydlowski Karen M |
author_facet |
Gerena Lucia Spadafora Carmenza Kopydlowski Karen M |
author_sort |
Gerena Lucia |
title |
Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation |
title_short |
Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation |
title_full |
Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation |
title_fullStr |
Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation |
title_full_unstemmed |
Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation |
title_sort |
comparison of the in vitro invasive capabilities of plasmodium falciparum schizonts isolated by percoll gradient or using magnetic based separation |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-96 https://doaj.org/article/682cfa92bae2420d8bbb9d2de1de1bad |
geographic |
Arctic |
geographic_facet |
Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 96 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/96 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-96 1475-2875 https://doaj.org/article/682cfa92bae2420d8bbb9d2de1de1bad |
op_doi |
https://doi.org/10.1186/1475-2875-10-96 |
container_title |
Malaria Journal |
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10 |
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1 |
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1766340055319707648 |