Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon

• Published in: Journal of Venomous Animals and Toxins including Tropical Diseases
• Main Authors: Alexandre do Rosário Casseb, Márcio Roberto Teixeira Nunes, Sueli Guerreiro Rodrigues, Elizabeth Salbé Travassos da Rosa, Livia Medeiros Neves Casseb, Samir Manssor Moraes Casseb, Sandro Patroca da Silva, Érika Dayane Leal Rodrigues, Pedro Fernando da Costa Vasconcelos
• Format: Article in Journal/Newspaper
• Language: English
• Published: SciELO 2014
• Subjects: Horses; Arboviruses; ELISA; Arctic medicine. Tropical medicine; RC955-962; Toxicology. Poisons; RA1190-1270; Zoology; QL1-991; Arctic
• Online Access: https://doi.org/10.1186/1678-9199-20-29; https://doaj.org/article/6825569b433c499ca4eeb5cb9e5f1703

Background The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutination inhibition test. A screening test assessed the conditional probability distribution and a Pearson linear correlation test determined the correlation strength among the absorbance values recorded for viruses from the same family.Findings Sensitivity varied between 40.85 and 100%; the specificity was low and ranged from 39.71 to 67.0%; and the accuracy varied between 41 and 65.2%. The test developed in this study yielded a large number of serological cross-reactions.Conclusions The test can be employed to detect IgG antibodies within one arbovirus family; however, the hemagglutination test or other more specific techniques, such as the serum neutralization test in mice or the plaque-reduction neutralization test, are essential complementary methods for positive cases.