Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies.
Metabolic fingerprinting analysis can offer insights into underlying reactions in a biological system; hence it is crucial to the understanding of disease pathogenesis and could provide useful tools for discovering biomarkers. We sought to examine the urine and plasma metabolome in individuals affec...
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ftdoajarticles:oai:doaj.org/article:680fe193205b4eb6a71401b4f105ba4e 2023-05-15T15:11:23+02:00 Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. Adewale S Adebayo Swapnil D Mundhe Henrietta O Awobode Olugbenga S Onile Atinuke M Agunloye Raphael D Isokpehi Yogesh S Shouche Bayatigeri Santhakumari Chiaka I Anumudu 2018-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006452 https://doaj.org/article/680fe193205b4eb6a71401b4f105ba4e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5945272?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006452 https://doaj.org/article/680fe193205b4eb6a71401b4f105ba4e PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006452 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006452 2022-12-31T14:40:46Z Metabolic fingerprinting analysis can offer insights into underlying reactions in a biological system; hence it is crucial to the understanding of disease pathogenesis and could provide useful tools for discovering biomarkers. We sought to examine the urine and plasma metabolome in individuals affected by urogenital schistosomiasis and its associated-bladder pathologies.Blood and midstream urine were obtained from volunteers who matched our inclusion criteria among residents from Eggua, southwestern Nigeria. Samples were screened by urinalysis, microscopy, PCR and ultrasonography, and categorised as advanced (urogenital schistosomiasis associated-bladder pathologies), infection-only (urogenital schistosomiasis alone) and controls (no infection and no pathology). Metabolites were extracted and data acquired with ultra high-performance liquid chromatography coupled with Thermo Q-Exactive orbitrap HRMS. Data was analysed with MetaboAnalyst, Workflow4Metabolomics, HMDB, LipidMaps and other bioinformatics tools, with univariate and multivariate statistics for metabolite selection.There were low levels of host sex steroids, and high levels of several benzenoids, catechols and lipids (including ganglioside, phosphatidylcholine and phosphatidylethanolamine), in infection-only and advanced cases (FDR<0.05, VIP>2, delta>2.0). Metabolites involved in biochemical pathways related to chorismate production were abundant in controls, while those related to choline and sphingolipid metabolism were upregulated in advanced cases (FDR<0.05). Some of these human host and Schistosoma haematobium molecules, including catechol estrogens, were good markers to distinguish infection-only and advanced cases.Altered glycerophospholipid and sphingolipid metabolism could be key factors promoting the development of bladder pathologies and tumours during urogenital schistosomiasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 4 e0006452 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Adewale S Adebayo Swapnil D Mundhe Henrietta O Awobode Olugbenga S Onile Atinuke M Agunloye Raphael D Isokpehi Yogesh S Shouche Bayatigeri Santhakumari Chiaka I Anumudu Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Metabolic fingerprinting analysis can offer insights into underlying reactions in a biological system; hence it is crucial to the understanding of disease pathogenesis and could provide useful tools for discovering biomarkers. We sought to examine the urine and plasma metabolome in individuals affected by urogenital schistosomiasis and its associated-bladder pathologies.Blood and midstream urine were obtained from volunteers who matched our inclusion criteria among residents from Eggua, southwestern Nigeria. Samples were screened by urinalysis, microscopy, PCR and ultrasonography, and categorised as advanced (urogenital schistosomiasis associated-bladder pathologies), infection-only (urogenital schistosomiasis alone) and controls (no infection and no pathology). Metabolites were extracted and data acquired with ultra high-performance liquid chromatography coupled with Thermo Q-Exactive orbitrap HRMS. Data was analysed with MetaboAnalyst, Workflow4Metabolomics, HMDB, LipidMaps and other bioinformatics tools, with univariate and multivariate statistics for metabolite selection.There were low levels of host sex steroids, and high levels of several benzenoids, catechols and lipids (including ganglioside, phosphatidylcholine and phosphatidylethanolamine), in infection-only and advanced cases (FDR<0.05, VIP>2, delta>2.0). Metabolites involved in biochemical pathways related to chorismate production were abundant in controls, while those related to choline and sphingolipid metabolism were upregulated in advanced cases (FDR<0.05). Some of these human host and Schistosoma haematobium molecules, including catechol estrogens, were good markers to distinguish infection-only and advanced cases.Altered glycerophospholipid and sphingolipid metabolism could be key factors promoting the development of bladder pathologies and tumours during urogenital schistosomiasis. |
format |
Article in Journal/Newspaper |
author |
Adewale S Adebayo Swapnil D Mundhe Henrietta O Awobode Olugbenga S Onile Atinuke M Agunloye Raphael D Isokpehi Yogesh S Shouche Bayatigeri Santhakumari Chiaka I Anumudu |
author_facet |
Adewale S Adebayo Swapnil D Mundhe Henrietta O Awobode Olugbenga S Onile Atinuke M Agunloye Raphael D Isokpehi Yogesh S Shouche Bayatigeri Santhakumari Chiaka I Anumudu |
author_sort |
Adewale S Adebayo |
title |
Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. |
title_short |
Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. |
title_full |
Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. |
title_fullStr |
Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. |
title_full_unstemmed |
Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. |
title_sort |
metabolite profiling for biomarkers in schistosoma haematobium infection and associated bladder pathologies. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006452 https://doaj.org/article/680fe193205b4eb6a71401b4f105ba4e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006452 (2018) |
op_relation |
http://europepmc.org/articles/PMC5945272?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006452 https://doaj.org/article/680fe193205b4eb6a71401b4f105ba4e |
op_doi |
https://doi.org/10.1371/journal.pntd.0006452 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
4 |
container_start_page |
e0006452 |
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1766342246274170880 |