Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis
Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradat...
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ftdoajarticles:oai:doaj.org/article:67fdea9f1eb44ad9b440a877852a81f9 2023-05-15T15:28:23+02:00 Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis Diego Pérez-Stuardo Jonathan Morales-Reyes Sebastián Tapia Diego E. Ahumada Allison Espinoza Valentina Soto-Herrera Bernardo Brianson Valentina Ibaceta Ana M. Sandino Eugenio Spencer Eva Vallejos-Vidal Felipe E. Reyes-López Jorge Valdés Sebastián Reyes-Cerpa 2019-03-01T00:00:00Z https://doi.org/10.3389/fimmu.2019.00434 https://doaj.org/article/67fdea9f1eb44ad9b440a877852a81f9 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2019.00434/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2019.00434 https://doaj.org/article/67fdea9f1eb44ad9b440a877852a81f9 Frontiers in Immunology, Vol 10 (2019) Atlantic salmon (Salmo salar) Piscirickettsiosis immune evasion mechanisms macrophages lysosome proteolytic activity Immunologic diseases. Allergy RC581-607 article 2019 ftdoajarticles https://doi.org/10.3389/fimmu.2019.00434 2022-12-31T10:35:58Z Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradation. However, the relevant aspects of the pathogenesis of P. salmonis as the host modulation that allow its intracellular survival have been poorly characterized. In this study, we evaluated the role of lysosomes in the response to P. salmonis infection in macrophage-enriched cell cultures established from Atlantic salmon head kidneys. Bacterial infection was confirmed using confocal microscopy. A gentamicin protection assay was performed to recover intracellular bacteria and the 16S rDNA copy number was quantified through quantitative polymerase chain reaction in order to determine the replication of P. salmonis within macrophages. Lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis was evaluated by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The results showed that P. salmonis can survive ≥120 h in Atlantic salmon macrophage-enriched cell cultures, accompanied by an increase in the detection of the 16S rDNA copy number/cell. The latter finding suggests that P. salmonis also replicates in Atlantic salmon macrophage-enriched cell cultures. Moreover, this bacterial survival and replication appears to be favored by a perturbation of the lysosomal degradation system. We observed a modulation in the total number of lysosomes and lysosomal acidification following infection with P. salmonis. Collectively, the results of this study showed that infection of Atlantic salmon macrophages with P. salmonis induced limited lysosomal response which may be associated with host immune evasion mechanisms of P. salmonis that have not been previously reported. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon (Salmo salar) Piscirickettsiosis immune evasion mechanisms macrophages lysosome proteolytic activity Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Atlantic salmon (Salmo salar) Piscirickettsiosis immune evasion mechanisms macrophages lysosome proteolytic activity Immunologic diseases. Allergy RC581-607 Diego Pérez-Stuardo Jonathan Morales-Reyes Sebastián Tapia Diego E. Ahumada Allison Espinoza Valentina Soto-Herrera Bernardo Brianson Valentina Ibaceta Ana M. Sandino Eugenio Spencer Eva Vallejos-Vidal Felipe E. Reyes-López Jorge Valdés Sebastián Reyes-Cerpa Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis |
topic_facet |
Atlantic salmon (Salmo salar) Piscirickettsiosis immune evasion mechanisms macrophages lysosome proteolytic activity Immunologic diseases. Allergy RC581-607 |
description |
Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradation. However, the relevant aspects of the pathogenesis of P. salmonis as the host modulation that allow its intracellular survival have been poorly characterized. In this study, we evaluated the role of lysosomes in the response to P. salmonis infection in macrophage-enriched cell cultures established from Atlantic salmon head kidneys. Bacterial infection was confirmed using confocal microscopy. A gentamicin protection assay was performed to recover intracellular bacteria and the 16S rDNA copy number was quantified through quantitative polymerase chain reaction in order to determine the replication of P. salmonis within macrophages. Lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis was evaluated by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The results showed that P. salmonis can survive ≥120 h in Atlantic salmon macrophage-enriched cell cultures, accompanied by an increase in the detection of the 16S rDNA copy number/cell. The latter finding suggests that P. salmonis also replicates in Atlantic salmon macrophage-enriched cell cultures. Moreover, this bacterial survival and replication appears to be favored by a perturbation of the lysosomal degradation system. We observed a modulation in the total number of lysosomes and lysosomal acidification following infection with P. salmonis. Collectively, the results of this study showed that infection of Atlantic salmon macrophages with P. salmonis induced limited lysosomal response which may be associated with host immune evasion mechanisms of P. salmonis that have not been previously reported. |
format |
Article in Journal/Newspaper |
author |
Diego Pérez-Stuardo Jonathan Morales-Reyes Sebastián Tapia Diego E. Ahumada Allison Espinoza Valentina Soto-Herrera Bernardo Brianson Valentina Ibaceta Ana M. Sandino Eugenio Spencer Eva Vallejos-Vidal Felipe E. Reyes-López Jorge Valdés Sebastián Reyes-Cerpa |
author_facet |
Diego Pérez-Stuardo Jonathan Morales-Reyes Sebastián Tapia Diego E. Ahumada Allison Espinoza Valentina Soto-Herrera Bernardo Brianson Valentina Ibaceta Ana M. Sandino Eugenio Spencer Eva Vallejos-Vidal Felipe E. Reyes-López Jorge Valdés Sebastián Reyes-Cerpa |
author_sort |
Diego Pérez-Stuardo |
title |
Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis |
title_short |
Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis |
title_full |
Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis |
title_fullStr |
Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis |
title_full_unstemmed |
Non-lysosomal Activation in Macrophages of Atlantic Salmon (Salmo salar) After Infection With Piscirickettsia salmonis |
title_sort |
non-lysosomal activation in macrophages of atlantic salmon (salmo salar) after infection with piscirickettsia salmonis |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fimmu.2019.00434 https://doaj.org/article/67fdea9f1eb44ad9b440a877852a81f9 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Immunology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2019.00434/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2019.00434 https://doaj.org/article/67fdea9f1eb44ad9b440a877852a81f9 |
op_doi |
https://doi.org/10.3389/fimmu.2019.00434 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
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1766358744374968320 |