Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding...
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ftdoajarticles:oai:doaj.org/article:67d0f7fa135a4ef78c558c975e00f142 2023-05-15T14:46:34+02:00 Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard Archana R. Meshram Anna Vader Svein Kristiansen Tove M. Gabrielsen 2017-06-01T00:00:00Z https://doi.org/10.3389/fmicb.2017.01099 https://doaj.org/article/67d0f7fa135a4ef78c558c975e00f142 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fmicb.2017.01099/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.01099 https://doaj.org/article/67d0f7fa135a4ef78c558c975e00f142 Frontiers in Microbiology, Vol 8 (2017) Arctic under-ice spring bloom microbial eukaryotes 18S V4 region pyrosequencing Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.3389/fmicb.2017.01099 2022-12-31T03:12:44Z Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding of DNA and RNA from the hypervariable V4 region of 18S nrDNA. At the two stations studied, the photosynthetic biomass was dominated by protists >3 μm and was concentrated in the upper 70–80 m, above the thermocline and halocline. Hierarchical cluster analyses as well as ordination analyses showed a distinct clustering of the microbial eukaryote communities according to a combination of water mass and local environmental characteristics. While samples collected in the surface mixed layer differed distinctly between the two sites, the deeper communities collected in Atlantic Water were fairly similar despite being geographically distant. The differentiation of the microbial eukaryote communities of the upper mixed water was probably driven by local development and advection, while the lack of such differentiation in the communities of Atlantic Water reflects the homogenizing effect of water currents on microbial communities. Article in Journal/Newspaper Arctic Svalbard Directory of Open Access Journals: DOAJ Articles Arctic Svalbard Frontiers in Microbiology 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic under-ice spring bloom microbial eukaryotes 18S V4 region pyrosequencing Microbiology QR1-502 |
spellingShingle |
Arctic under-ice spring bloom microbial eukaryotes 18S V4 region pyrosequencing Microbiology QR1-502 Archana R. Meshram Anna Vader Svein Kristiansen Tove M. Gabrielsen Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard |
topic_facet |
Arctic under-ice spring bloom microbial eukaryotes 18S V4 region pyrosequencing Microbiology QR1-502 |
description |
Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding of DNA and RNA from the hypervariable V4 region of 18S nrDNA. At the two stations studied, the photosynthetic biomass was dominated by protists >3 μm and was concentrated in the upper 70–80 m, above the thermocline and halocline. Hierarchical cluster analyses as well as ordination analyses showed a distinct clustering of the microbial eukaryote communities according to a combination of water mass and local environmental characteristics. While samples collected in the surface mixed layer differed distinctly between the two sites, the deeper communities collected in Atlantic Water were fairly similar despite being geographically distant. The differentiation of the microbial eukaryote communities of the upper mixed water was probably driven by local development and advection, while the lack of such differentiation in the communities of Atlantic Water reflects the homogenizing effect of water currents on microbial communities. |
format |
Article in Journal/Newspaper |
author |
Archana R. Meshram Anna Vader Svein Kristiansen Tove M. Gabrielsen |
author_facet |
Archana R. Meshram Anna Vader Svein Kristiansen Tove M. Gabrielsen |
author_sort |
Archana R. Meshram |
title |
Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard |
title_short |
Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard |
title_full |
Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard |
title_fullStr |
Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard |
title_full_unstemmed |
Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard |
title_sort |
microbial eukaryotes in an arctic under-ice spring bloom north of svalbard |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fmicb.2017.01099 https://doaj.org/article/67d0f7fa135a4ef78c558c975e00f142 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_source |
Frontiers in Microbiology, Vol 8 (2017) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01099/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.01099 https://doaj.org/article/67d0f7fa135a4ef78c558c975e00f142 |
op_doi |
https://doi.org/10.3389/fmicb.2017.01099 |
container_title |
Frontiers in Microbiology |
container_volume |
8 |
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1766317780294959104 |