Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.)
DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repre...
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ftdoajarticles:oai:doaj.org/article:6788d64caae343d9bb819e617b0ec1d9 2023-05-15T16:02:00+02:00 Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana 2022-07-01T00:00:00Z https://doi.org/10.3389/fgene.2022.894020 https://doaj.org/article/6788d64caae343d9bb819e617b0ec1d9 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fgene.2022.894020/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2022.894020 https://doaj.org/article/6788d64caae343d9bb819e617b0ec1d9 Frontiers in Genetics, Vol 13 (2022) DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3389/fgene.2022.894020 2022-12-30T23:16:43Z DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repressor of silencing 1 (ROS1) and DEMETER-Like (DML). The TadMTases in the same subfamily or clade in the phylogenetic tree have similar gene structures, protein motifs, and domains. The promoter sequence contains multiple cis-regulatory elements (CREs) that respond to abiotic stress, hormones, and light, suggesting that the majority of TadMTase genes play a role in wheat growth, development, and stress response. The nuclear localization signals (NLSs), subcellular localization, and SRR motifs were also analyzed. The expression profile analyses revealed that TadMTase genes showed differential gene expression patterns in distinct developmental stages and tissues as well as under heat stress (HS). Furthermore, the qRT-PCR analysis revealed that TadMTase gene expression differed amongst wheat cultivars with varying degrees of HS tolerance. Overall, this work contributes to the understanding of the biological function of wheat dMTases and lays the foundation for future investigations. Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles Frontiers in Genetics 13 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) Genetics QH426-470 |
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DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) Genetics QH426-470 Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
topic_facet |
DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) Genetics QH426-470 |
description |
DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repressor of silencing 1 (ROS1) and DEMETER-Like (DML). The TadMTases in the same subfamily or clade in the phylogenetic tree have similar gene structures, protein motifs, and domains. The promoter sequence contains multiple cis-regulatory elements (CREs) that respond to abiotic stress, hormones, and light, suggesting that the majority of TadMTase genes play a role in wheat growth, development, and stress response. The nuclear localization signals (NLSs), subcellular localization, and SRR motifs were also analyzed. The expression profile analyses revealed that TadMTase genes showed differential gene expression patterns in distinct developmental stages and tissues as well as under heat stress (HS). Furthermore, the qRT-PCR analysis revealed that TadMTase gene expression differed amongst wheat cultivars with varying degrees of HS tolerance. Overall, this work contributes to the understanding of the biological function of wheat dMTases and lays the foundation for future investigations. |
format |
Article in Journal/Newspaper |
author |
Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana |
author_facet |
Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana |
author_sort |
Vijay Gahlaut |
title |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_short |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_full |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_fullStr |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_full_unstemmed |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_sort |
identification and characterization of dna demethylase genes and their association with thermal stress in wheat (triticum aestivum l.) |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fgene.2022.894020 https://doaj.org/article/6788d64caae343d9bb819e617b0ec1d9 |
genre |
DML |
genre_facet |
DML |
op_source |
Frontiers in Genetics, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fgene.2022.894020/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2022.894020 https://doaj.org/article/6788d64caae343d9bb819e617b0ec1d9 |
op_doi |
https://doi.org/10.3389/fgene.2022.894020 |
container_title |
Frontiers in Genetics |
container_volume |
13 |
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1766397649674567680 |