1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators.
Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/reperfusion (I/R) results in a disruption of cellular metabolic processes that ulti...
Published in: | PLoS ONE |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0107493 https://doaj.org/article/678318bdbbc44a4e97bafa4db5990323 |
id |
ftdoajarticles:oai:doaj.org/article:678318bdbbc44a4e97bafa4db5990323 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:678318bdbbc44a4e97bafa4db5990323 2023-05-15T15:16:48+02:00 1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. Lori K Bogren Carl J Murphy Erin L Johnston Neeraj Sinha Natalie J Serkova Kelly L Drew 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0107493 https://doaj.org/article/678318bdbbc44a4e97bafa4db5990323 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4161479?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0107493 https://doaj.org/article/678318bdbbc44a4e97bafa4db5990323 PLoS ONE, Vol 9, Iss 9, p e107493 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0107493 2022-12-31T02:26:47Z Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/reperfusion (I/R) results in a disruption of cellular metabolic processes that ultimately lead to tissue and organ dysfunction or failure. Resistance to I/R injury is a characteristic of hibernating mammals. The present study sought to identify circulating metabolites in the rat as biomarkers for metabolic alterations associated with poor outcome after HS. Arctic ground squirrels (AGS), a hibernating species that resists I/R injury independent of decreased body temperature (warm I/R), was used as a negative control.Male Sprague-Dawley rats and AGS were subject to HS by withdrawing blood to a mean arterial pressure (MAP) of 35 mmHg and maintaining the low MAP for 20 min before reperfusing with Ringers. The animals' temperature was maintained at 37 ± 0.5 °C for the duration of the experiment. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion. Hydrophilic and lipid metabolites from plasma were then analyzed via 1H-NMR from unprocessed plasma and lipid extracts, respectively. Rats, susceptible to I/R injury, had a qualitative shift in their hydrophilic metabolic fingerprint including differential activation of glucose and anaerobic metabolism and had alterations in several metabolites during I/R indicative of metabolic adjustments and organ damage. In contrast, I/R injury resistant AGS, regardless of season or body temperature, maintained a stable metabolic homeostasis revealed by a qualitative 1H-NMR metabolic profile with few changes in quantified metabolites during HS-induced global I/R.An increase in circulating metabolites indicative of anaerobic metabolism and activation of glycolytic pathways is associated with poor prognosis after HS in rats. These same biomarkers are absent in AGS after HS with warm I/R. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS ONE 9 9 e107493 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Lori K Bogren Carl J Murphy Erin L Johnston Neeraj Sinha Natalie J Serkova Kelly L Drew 1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
topic_facet |
Medicine R Science Q |
description |
Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/reperfusion (I/R) results in a disruption of cellular metabolic processes that ultimately lead to tissue and organ dysfunction or failure. Resistance to I/R injury is a characteristic of hibernating mammals. The present study sought to identify circulating metabolites in the rat as biomarkers for metabolic alterations associated with poor outcome after HS. Arctic ground squirrels (AGS), a hibernating species that resists I/R injury independent of decreased body temperature (warm I/R), was used as a negative control.Male Sprague-Dawley rats and AGS were subject to HS by withdrawing blood to a mean arterial pressure (MAP) of 35 mmHg and maintaining the low MAP for 20 min before reperfusing with Ringers. The animals' temperature was maintained at 37 ± 0.5 °C for the duration of the experiment. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion. Hydrophilic and lipid metabolites from plasma were then analyzed via 1H-NMR from unprocessed plasma and lipid extracts, respectively. Rats, susceptible to I/R injury, had a qualitative shift in their hydrophilic metabolic fingerprint including differential activation of glucose and anaerobic metabolism and had alterations in several metabolites during I/R indicative of metabolic adjustments and organ damage. In contrast, I/R injury resistant AGS, regardless of season or body temperature, maintained a stable metabolic homeostasis revealed by a qualitative 1H-NMR metabolic profile with few changes in quantified metabolites during HS-induced global I/R.An increase in circulating metabolites indicative of anaerobic metabolism and activation of glycolytic pathways is associated with poor prognosis after HS in rats. These same biomarkers are absent in AGS after HS with warm I/R. |
format |
Article in Journal/Newspaper |
author |
Lori K Bogren Carl J Murphy Erin L Johnston Neeraj Sinha Natalie J Serkova Kelly L Drew |
author_facet |
Lori K Bogren Carl J Murphy Erin L Johnston Neeraj Sinha Natalie J Serkova Kelly L Drew |
author_sort |
Lori K Bogren |
title |
1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
title_short |
1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
title_full |
1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
title_fullStr |
1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
title_full_unstemmed |
1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
title_sort |
1h-nmr metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0107493 https://doaj.org/article/678318bdbbc44a4e97bafa4db5990323 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS ONE, Vol 9, Iss 9, p e107493 (2014) |
op_relation |
http://europepmc.org/articles/PMC4161479?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0107493 https://doaj.org/article/678318bdbbc44a4e97bafa4db5990323 |
op_doi |
https://doi.org/10.1371/journal.pone.0107493 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
9 |
container_start_page |
e107493 |
_version_ |
1766347090327240704 |