Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors
ABSTRACT Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health, but it also represen...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:676e5652c9064e95905523810fc6eefd 2024-09-09T19:25:37+00:00 Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors Márcia Oliveira Casotti Ronaldo Cesar Borges Gryschek Fabiana Martins de Paula Michele Gomes-Gouvêa João Renato Rebello Pinho Roseli Tuan Emmanuel Dias-Neto Expedito José de Albuquerque Luna Maria Cristina Carvalho do Espírito-Santo 2020-03-01T00:00:00Z https://doi.org/10.1590/s1678-9946202062017 https://doaj.org/article/676e5652c9064e95905523810fc6eefd EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100209&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062017 https://doaj.org/article/676e5652c9064e95905523810fc6eefd Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) Schistosoma mansoni Biomphalaria glabrata Molecular diagnosis Surveillance Snail Vector Polymerase Chain Reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1590/s1678-9946202062017 2024-08-05T17:49:31Z ABSTRACT Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health, but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 62 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Schistosoma mansoni Biomphalaria glabrata Molecular diagnosis Surveillance Snail Vector Polymerase Chain Reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Schistosoma mansoni Biomphalaria glabrata Molecular diagnosis Surveillance Snail Vector Polymerase Chain Reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Márcia Oliveira Casotti Ronaldo Cesar Borges Gryschek Fabiana Martins de Paula Michele Gomes-Gouvêa João Renato Rebello Pinho Roseli Tuan Emmanuel Dias-Neto Expedito José de Albuquerque Luna Maria Cristina Carvalho do Espírito-Santo Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors |
topic_facet |
Schistosoma mansoni Biomphalaria glabrata Molecular diagnosis Surveillance Snail Vector Polymerase Chain Reaction Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health, but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission. |
format |
Article in Journal/Newspaper |
author |
Márcia Oliveira Casotti Ronaldo Cesar Borges Gryschek Fabiana Martins de Paula Michele Gomes-Gouvêa João Renato Rebello Pinho Roseli Tuan Emmanuel Dias-Neto Expedito José de Albuquerque Luna Maria Cristina Carvalho do Espírito-Santo |
author_facet |
Márcia Oliveira Casotti Ronaldo Cesar Borges Gryschek Fabiana Martins de Paula Michele Gomes-Gouvêa João Renato Rebello Pinho Roseli Tuan Emmanuel Dias-Neto Expedito José de Albuquerque Luna Maria Cristina Carvalho do Espírito-Santo |
author_sort |
Márcia Oliveira Casotti |
title |
Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors |
title_short |
Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors |
title_full |
Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors |
title_fullStr |
Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors |
title_full_unstemmed |
Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors |
title_sort |
molecular detection of prepatent schistosoma mansoni infection in biomphalaria glabrata snail vectors |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2020 |
url |
https://doi.org/10.1590/s1678-9946202062017 https://doaj.org/article/676e5652c9064e95905523810fc6eefd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100209&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062017 https://doaj.org/article/676e5652c9064e95905523810fc6eefd |
op_doi |
https://doi.org/10.1590/s1678-9946202062017 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
62 |
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