Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates
Abstract Background Despite years of effort, a licensed malaria vaccine is not yet available. One of the obstacles facing the development of a malaria vaccine is the extensive heterogeneity of many of the current malaria vaccine antigens. To counteract this antigenic diversity, an effective malaria...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-10-65 https://doaj.org/article/674efcc5a4194a5b8bf1e383490815b3 |
id |
ftdoajarticles:oai:doaj.org/article:674efcc5a4194a5b8bf1e383490815b3 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:674efcc5a4194a5b8bf1e383490815b3 2023-05-15T15:13:46+02:00 Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates Patterson Noelle Gowda Kalpana Aguiar Joao Limbach Keith Abot Esteban Sedegah Martha Sacci John Richie Thomas 2011-03-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-65 https://doaj.org/article/674efcc5a4194a5b8bf1e383490815b3 EN eng BMC http://www.malariajournal.com/content/10/1/65 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-65 1475-2875 https://doaj.org/article/674efcc5a4194a5b8bf1e383490815b3 Malaria Journal, Vol 10, Iss 1, p 65 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-65 2022-12-31T08:34:11Z Abstract Background Despite years of effort, a licensed malaria vaccine is not yet available. One of the obstacles facing the development of a malaria vaccine is the extensive heterogeneity of many of the current malaria vaccine antigens. To counteract this antigenic diversity, an effective malaria vaccine may need to elicit an immune response against multiple malaria antigens, thereby limiting the negative impact of variability in any one antigen. Since most of the malaria vaccine antigens that have been evaluated in people have not elicited a protective immune response, there is a need to identify additional protective antigens. In this study, the efficacy of three pre-erythrocytic stage malaria antigens was evaluated in a Plasmodium yoelii /mouse protection model. Methods Mice were immunized with plasmid DNA and vaccinia virus vectors that expressed one, two or all three P. yoelii vaccine antigens. The immunized mice were challenged with 300 P. yoelii sporozoites and evaluated for subsequent infection. Results Vaccines that expressed any one of the three antigens did not protect a high percentage of mice against a P. yoelii challenge. However, vaccines that expressed all three antigens protected a higher percentage of mice than a vaccine that expressed PyCSP, the most efficacious malaria vaccine antigen. Dissection of the multi-antigen vaccine indicated that protection was primarily associated with two of the three P. yoelii antigens. The protection elicited by a vaccine expressing these two antigens exceeded the sum of the protection elicited by the single antigen vaccines, suggesting a potential synergistic interaction. Conclusions This work identifies two promising malaria vaccine antigen candidates and suggests that a multi-antigen vaccine may be more efficacious than a single antigen vaccine. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Patterson Noelle Gowda Kalpana Aguiar Joao Limbach Keith Abot Esteban Sedegah Martha Sacci John Richie Thomas Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Despite years of effort, a licensed malaria vaccine is not yet available. One of the obstacles facing the development of a malaria vaccine is the extensive heterogeneity of many of the current malaria vaccine antigens. To counteract this antigenic diversity, an effective malaria vaccine may need to elicit an immune response against multiple malaria antigens, thereby limiting the negative impact of variability in any one antigen. Since most of the malaria vaccine antigens that have been evaluated in people have not elicited a protective immune response, there is a need to identify additional protective antigens. In this study, the efficacy of three pre-erythrocytic stage malaria antigens was evaluated in a Plasmodium yoelii /mouse protection model. Methods Mice were immunized with plasmid DNA and vaccinia virus vectors that expressed one, two or all three P. yoelii vaccine antigens. The immunized mice were challenged with 300 P. yoelii sporozoites and evaluated for subsequent infection. Results Vaccines that expressed any one of the three antigens did not protect a high percentage of mice against a P. yoelii challenge. However, vaccines that expressed all three antigens protected a higher percentage of mice than a vaccine that expressed PyCSP, the most efficacious malaria vaccine antigen. Dissection of the multi-antigen vaccine indicated that protection was primarily associated with two of the three P. yoelii antigens. The protection elicited by a vaccine expressing these two antigens exceeded the sum of the protection elicited by the single antigen vaccines, suggesting a potential synergistic interaction. Conclusions This work identifies two promising malaria vaccine antigen candidates and suggests that a multi-antigen vaccine may be more efficacious than a single antigen vaccine. |
format |
Article in Journal/Newspaper |
author |
Patterson Noelle Gowda Kalpana Aguiar Joao Limbach Keith Abot Esteban Sedegah Martha Sacci John Richie Thomas |
author_facet |
Patterson Noelle Gowda Kalpana Aguiar Joao Limbach Keith Abot Esteban Sedegah Martha Sacci John Richie Thomas |
author_sort |
Patterson Noelle |
title |
Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
title_short |
Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
title_full |
Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
title_fullStr |
Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
title_full_unstemmed |
Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
title_sort |
identification of two new protective pre-erythrocytic malaria vaccine antigen candidates |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-65 https://doaj.org/article/674efcc5a4194a5b8bf1e383490815b3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 65 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/65 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-65 1475-2875 https://doaj.org/article/674efcc5a4194a5b8bf1e383490815b3 |
op_doi |
https://doi.org/10.1186/1475-2875-10-65 |
container_title |
Malaria Journal |
container_volume |
10 |
container_issue |
1 |
_version_ |
1766344291340255232 |