Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India.
Cholera is an acute diarrheal disease and a major public health problem in many developing countries in Asia, Africa, and Latin America. Since the Bay of Bengal is considered the epicenter for the seventh cholera pandemic, it is important to understand the genetic dynamism of Vibrio cholerae from Ko...
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ftdoajarticles:oai:doaj.org/article:673c1f212fe54fe89c5bae33d22f7c24 2023-05-15T15:13:34+02:00 Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. Daisuke Imamura Masatomo Morita Tsuyoshi Sekizuka Tamaki Mizuno Taichiro Takemura Tetsu Yamashiro Goutam Chowdhury Gururaja P Pazhani Asish K Mukhopadhyay Thandavarayan Ramamurthy Shin-Ichi Miyoshi Makoto Kuroda Sumio Shinoda Makoto Ohnishi 2017-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005386 https://doaj.org/article/673c1f212fe54fe89c5bae33d22f7c24 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5349696?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005386 https://doaj.org/article/673c1f212fe54fe89c5bae33d22f7c24 PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005386 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005386 2022-12-31T16:03:27Z Cholera is an acute diarrheal disease and a major public health problem in many developing countries in Asia, Africa, and Latin America. Since the Bay of Bengal is considered the epicenter for the seventh cholera pandemic, it is important to understand the genetic dynamism of Vibrio cholerae from Kolkata, as a representative of the Bengal region. We analyzed whole genome sequence data of V. cholerae O1 isolated from cholera patients in Kolkata, India, from 2007 to 2014 and identified the heterogeneous genomic region in these strains. In addition, we carried out a phylogenetic analysis based on the whole genome single nucleotide polymorphisms to determine the genetic lineage of strains in Kolkata. This analysis revealed the heterogeneity of the Vibrio seventh pandemic island (VSP)-II in Kolkata strains. The ctxB genotype was also heterogeneous and was highly related to VSP-II types. In addition, phylogenetic analysis revealed the shifts in predominant strains in Kolkata. Two distinct lineages, 1 and 2, were found between 2007 and 2010. However, the proportion changed markedly in 2010 and lineage 2 strains were predominant thereafter. Lineage 2 can be divided into four sublineages, I, II, III and IV. The results of this study indicate that lineages 1 and 2-I were concurrently prevalent between 2007 and 2009, and lineage 2-III observed in 2010, followed by the predominance of lineage 2-IV in 2011 and continued until 2014. Our findings demonstrate that the epidemic of cholera in Kolkata was caused by several distinct strains that have been constantly changing within the genetic lineages of V. cholerae O1 in recent years. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 2 e0005386 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daisuke Imamura Masatomo Morita Tsuyoshi Sekizuka Tamaki Mizuno Taichiro Takemura Tetsu Yamashiro Goutam Chowdhury Gururaja P Pazhani Asish K Mukhopadhyay Thandavarayan Ramamurthy Shin-Ichi Miyoshi Makoto Kuroda Sumio Shinoda Makoto Ohnishi Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cholera is an acute diarrheal disease and a major public health problem in many developing countries in Asia, Africa, and Latin America. Since the Bay of Bengal is considered the epicenter for the seventh cholera pandemic, it is important to understand the genetic dynamism of Vibrio cholerae from Kolkata, as a representative of the Bengal region. We analyzed whole genome sequence data of V. cholerae O1 isolated from cholera patients in Kolkata, India, from 2007 to 2014 and identified the heterogeneous genomic region in these strains. In addition, we carried out a phylogenetic analysis based on the whole genome single nucleotide polymorphisms to determine the genetic lineage of strains in Kolkata. This analysis revealed the heterogeneity of the Vibrio seventh pandemic island (VSP)-II in Kolkata strains. The ctxB genotype was also heterogeneous and was highly related to VSP-II types. In addition, phylogenetic analysis revealed the shifts in predominant strains in Kolkata. Two distinct lineages, 1 and 2, were found between 2007 and 2010. However, the proportion changed markedly in 2010 and lineage 2 strains were predominant thereafter. Lineage 2 can be divided into four sublineages, I, II, III and IV. The results of this study indicate that lineages 1 and 2-I were concurrently prevalent between 2007 and 2009, and lineage 2-III observed in 2010, followed by the predominance of lineage 2-IV in 2011 and continued until 2014. Our findings demonstrate that the epidemic of cholera in Kolkata was caused by several distinct strains that have been constantly changing within the genetic lineages of V. cholerae O1 in recent years. |
format |
Article in Journal/Newspaper |
author |
Daisuke Imamura Masatomo Morita Tsuyoshi Sekizuka Tamaki Mizuno Taichiro Takemura Tetsu Yamashiro Goutam Chowdhury Gururaja P Pazhani Asish K Mukhopadhyay Thandavarayan Ramamurthy Shin-Ichi Miyoshi Makoto Kuroda Sumio Shinoda Makoto Ohnishi |
author_facet |
Daisuke Imamura Masatomo Morita Tsuyoshi Sekizuka Tamaki Mizuno Taichiro Takemura Tetsu Yamashiro Goutam Chowdhury Gururaja P Pazhani Asish K Mukhopadhyay Thandavarayan Ramamurthy Shin-Ichi Miyoshi Makoto Kuroda Sumio Shinoda Makoto Ohnishi |
author_sort |
Daisuke Imamura |
title |
Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. |
title_short |
Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. |
title_full |
Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. |
title_fullStr |
Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. |
title_full_unstemmed |
Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. |
title_sort |
comparative genome analysis of vsp-ii and snps reveals heterogenic variation in contemporary strains of vibrio cholerae o1 isolated from cholera patients in kolkata, india. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005386 https://doaj.org/article/673c1f212fe54fe89c5bae33d22f7c24 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005386 (2017) |
op_relation |
http://europepmc.org/articles/PMC5349696?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005386 https://doaj.org/article/673c1f212fe54fe89c5bae33d22f7c24 |
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https://doi.org/10.1371/journal.pntd.0005386 |
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PLOS Neglected Tropical Diseases |
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11 |
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e0005386 |
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