Distribution of Calanus spp. as determined using a genetic identification system
The morphological similarity of Calanus species necessitates that the only unambiguous way of discriminating between the different species at any developmental stage is with molecular tools. We have developed a simple molecular technique to distinguish between the four species of Calanus copepods fo...
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Consejo Superior de Investigaciones Científicas
2004
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Online Access: | https://doi.org/10.3989/scimar.2004.68s1121 https://doaj.org/article/67163ee162b5497b83196e9daa7f3272 |
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ftdoajarticles:oai:doaj.org/article:67163ee162b5497b83196e9daa7f3272 2023-05-15T17:30:23+02:00 Distribution of Calanus spp. as determined using a genetic identification system Penelope K. Lindeque Rober P. Harris Malcolm B. Jones Gary R. Smerdon 2004-04-01T00:00:00Z https://doi.org/10.3989/scimar.2004.68s1121 https://doaj.org/article/67163ee162b5497b83196e9daa7f3272 EN eng Consejo Superior de Investigaciones Científicas http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/392 https://doaj.org/toc/0214-8358 https://doaj.org/toc/1886-8134 0214-8358 1886-8134 doi:10.3989/scimar.2004.68s1121 https://doaj.org/article/67163ee162b5497b83196e9daa7f3272 Scientia Marina, Vol 68, Iss S1, Pp 121-128 (2004) calanus 16s rrna mtdna rflp genetic identification distribution Aquaculture. Fisheries. Angling SH1-691 article 2004 ftdoajarticles https://doi.org/10.3989/scimar.2004.68s1121 2022-12-31T09:37:42Z The morphological similarity of Calanus species necessitates that the only unambiguous way of discriminating between the different species at any developmental stage is with molecular tools. We have developed a simple molecular technique to distinguish between the four species of Calanus copepods found in the North Atlantic (Calanus helgolandicus, C. finmarchicus, C. glacialis and C. hyperboreus) at any life stage. This system involves the PCR amplification of a region of the 16S rRNA gene, followed by Restriction Fragment Length Polymorphism (RFLP) analysis of the amplified product. This paper describes the application of the technique to a number of Calanus samples taken from a wide geographical range within the North Atlantic. Samples were acquired from sites ranging in latitude from the English Channel (50°N) to Tromsø (69°N), including many regions of co-occurrence. A comparison has been made between the distribution of Calanus as determined by molecular techniques and distributions established using traditional morphological identification. The molecular analysis has clearly shown extended areas of distribution and co-occurrence and has confirmed the intraspecific conservation of the restriction sites and therefore the reliability of the technique. Article in Journal/Newspaper North Atlantic Tromsø Copepods Directory of Open Access Journals: DOAJ Articles Tromsø Scientia Marina 68 S1 121 128 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
calanus 16s rrna mtdna rflp genetic identification distribution Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
calanus 16s rrna mtdna rflp genetic identification distribution Aquaculture. Fisheries. Angling SH1-691 Penelope K. Lindeque Rober P. Harris Malcolm B. Jones Gary R. Smerdon Distribution of Calanus spp. as determined using a genetic identification system |
topic_facet |
calanus 16s rrna mtdna rflp genetic identification distribution Aquaculture. Fisheries. Angling SH1-691 |
description |
The morphological similarity of Calanus species necessitates that the only unambiguous way of discriminating between the different species at any developmental stage is with molecular tools. We have developed a simple molecular technique to distinguish between the four species of Calanus copepods found in the North Atlantic (Calanus helgolandicus, C. finmarchicus, C. glacialis and C. hyperboreus) at any life stage. This system involves the PCR amplification of a region of the 16S rRNA gene, followed by Restriction Fragment Length Polymorphism (RFLP) analysis of the amplified product. This paper describes the application of the technique to a number of Calanus samples taken from a wide geographical range within the North Atlantic. Samples were acquired from sites ranging in latitude from the English Channel (50°N) to Tromsø (69°N), including many regions of co-occurrence. A comparison has been made between the distribution of Calanus as determined by molecular techniques and distributions established using traditional morphological identification. The molecular analysis has clearly shown extended areas of distribution and co-occurrence and has confirmed the intraspecific conservation of the restriction sites and therefore the reliability of the technique. |
format |
Article in Journal/Newspaper |
author |
Penelope K. Lindeque Rober P. Harris Malcolm B. Jones Gary R. Smerdon |
author_facet |
Penelope K. Lindeque Rober P. Harris Malcolm B. Jones Gary R. Smerdon |
author_sort |
Penelope K. Lindeque |
title |
Distribution of Calanus spp. as determined using a genetic identification system |
title_short |
Distribution of Calanus spp. as determined using a genetic identification system |
title_full |
Distribution of Calanus spp. as determined using a genetic identification system |
title_fullStr |
Distribution of Calanus spp. as determined using a genetic identification system |
title_full_unstemmed |
Distribution of Calanus spp. as determined using a genetic identification system |
title_sort |
distribution of calanus spp. as determined using a genetic identification system |
publisher |
Consejo Superior de Investigaciones Científicas |
publishDate |
2004 |
url |
https://doi.org/10.3989/scimar.2004.68s1121 https://doaj.org/article/67163ee162b5497b83196e9daa7f3272 |
geographic |
Tromsø |
geographic_facet |
Tromsø |
genre |
North Atlantic Tromsø Copepods |
genre_facet |
North Atlantic Tromsø Copepods |
op_source |
Scientia Marina, Vol 68, Iss S1, Pp 121-128 (2004) |
op_relation |
http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/392 https://doaj.org/toc/0214-8358 https://doaj.org/toc/1886-8134 0214-8358 1886-8134 doi:10.3989/scimar.2004.68s1121 https://doaj.org/article/67163ee162b5497b83196e9daa7f3272 |
op_doi |
https://doi.org/10.3989/scimar.2004.68s1121 |
container_title |
Scientia Marina |
container_volume |
68 |
container_issue |
S1 |
container_start_page |
121 |
op_container_end_page |
128 |
_version_ |
1766126760895709184 |