Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border
Abstract Background Few studies have been conducted to investigate the distribution of mosquito vectors and the population structure of secondary vectors in the border region of Cambodia-Laos. The aim of this work was to study the mosquito diversity and molecular phylogeny of secondary vectors, i.e....
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ftdoajarticles:oai:doaj.org/article:6710227f026d4dc1a3f0419a43fbcddd 2023-05-15T15:16:44+02:00 Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border Yilong Zhang Canglin Zhang Rui Yang Chunhai Luo Yan Deng Yan Liu Hongning Zhou Dongmei Zhang 2022-03-01T00:00:00Z https://doi.org/10.1186/s12936-022-04121-w https://doaj.org/article/6710227f026d4dc1a3f0419a43fbcddd EN eng BMC https://doi.org/10.1186/s12936-022-04121-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04121-w 1475-2875 https://doaj.org/article/6710227f026d4dc1a3f0419a43fbcddd Malaria Journal, Vol 21, Iss 1, Pp 1-12 (2022) Malaria Mosquitoes COII Molecular phylogeny Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04121-w 2022-12-31T07:12:21Z Abstract Background Few studies have been conducted to investigate the distribution of mosquito vectors and the population structure of secondary vectors in the border region of Cambodia-Laos. The aim of this work was to study the mosquito diversity and molecular phylogeny of secondary vectors, i.e., Anopheles nivipes in this area. Methods 1440 adult mosquitoes were trapped in the Cambodia-Laos border. mtDNA-COII were amplified and sequenced from 53 An. nivipes DNA samples. Together with COII sequences deposited in GenBank, a total of 86 COII sequences were used for examining population variations, genetic differentiation, spatial population structure, population expansion, and gene flow patterns. Results The adult mosquitoes were classified into 5 genera and 27 species in this border region. The predominant genera were Culex (60.07%, 865/1440) and Anopheles (31.25%, 450/1440), and the major Anopheles species were An. nivipes (73.56%, 331/450) and Anopheles maculatus (14.22%, 64/450). Based on sequences analysis of COII, a high level of genetic differentiation was reported in two Northwest India (Cheema and Bathinda, Punjab) and Cambodia-Laos (Siem Pang, Stung treng) populations (F ST = 0.97824, 0.97343, P < 0.05), as well as lower gene flow (Nm = 0.01112, 0.01365) in the An. nivipes populations. Phylogenetic analysis and SAMOVA revealed a gene barrier restricting gene flow among three An. nivipes populations. Mantel test suggested a significant correlation between geography and gene distance in all An. nivipes populations (Z = 44,983.1865, r = 0.5575, P = 0.0070). Neutrality test and Mismatch distribution revealed a recent population expansion of An. nivipes in the Cambodia-Laos population. Conclusions Anopheles nivipes was one of the major Anopheles species in the Cambodia-Laos border. Based on sequences analysis of COII, a genetic barrier between Cambodia-Laos and two Indian populations was found, and a recent population expanding or selecting of An. nivipes occurred in the Cambodia-Laos population, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Siem ENVELOPE(7.559,7.559,62.581,62.581) Malaria Journal 21 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Mosquitoes COII Molecular phylogeny Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Mosquitoes COII Molecular phylogeny Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yilong Zhang Canglin Zhang Rui Yang Chunhai Luo Yan Deng Yan Liu Hongning Zhou Dongmei Zhang Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border |
topic_facet |
Malaria Mosquitoes COII Molecular phylogeny Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Few studies have been conducted to investigate the distribution of mosquito vectors and the population structure of secondary vectors in the border region of Cambodia-Laos. The aim of this work was to study the mosquito diversity and molecular phylogeny of secondary vectors, i.e., Anopheles nivipes in this area. Methods 1440 adult mosquitoes were trapped in the Cambodia-Laos border. mtDNA-COII were amplified and sequenced from 53 An. nivipes DNA samples. Together with COII sequences deposited in GenBank, a total of 86 COII sequences were used for examining population variations, genetic differentiation, spatial population structure, population expansion, and gene flow patterns. Results The adult mosquitoes were classified into 5 genera and 27 species in this border region. The predominant genera were Culex (60.07%, 865/1440) and Anopheles (31.25%, 450/1440), and the major Anopheles species were An. nivipes (73.56%, 331/450) and Anopheles maculatus (14.22%, 64/450). Based on sequences analysis of COII, a high level of genetic differentiation was reported in two Northwest India (Cheema and Bathinda, Punjab) and Cambodia-Laos (Siem Pang, Stung treng) populations (F ST = 0.97824, 0.97343, P < 0.05), as well as lower gene flow (Nm = 0.01112, 0.01365) in the An. nivipes populations. Phylogenetic analysis and SAMOVA revealed a gene barrier restricting gene flow among three An. nivipes populations. Mantel test suggested a significant correlation between geography and gene distance in all An. nivipes populations (Z = 44,983.1865, r = 0.5575, P = 0.0070). Neutrality test and Mismatch distribution revealed a recent population expansion of An. nivipes in the Cambodia-Laos population. Conclusions Anopheles nivipes was one of the major Anopheles species in the Cambodia-Laos border. Based on sequences analysis of COII, a genetic barrier between Cambodia-Laos and two Indian populations was found, and a recent population expanding or selecting of An. nivipes occurred in the Cambodia-Laos population, ... |
format |
Article in Journal/Newspaper |
author |
Yilong Zhang Canglin Zhang Rui Yang Chunhai Luo Yan Deng Yan Liu Hongning Zhou Dongmei Zhang |
author_facet |
Yilong Zhang Canglin Zhang Rui Yang Chunhai Luo Yan Deng Yan Liu Hongning Zhou Dongmei Zhang |
author_sort |
Yilong Zhang |
title |
Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border |
title_short |
Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border |
title_full |
Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border |
title_fullStr |
Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border |
title_full_unstemmed |
Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border |
title_sort |
molecular phylogeny of anopheles nivipes based on mtdna-coii and mosquito diversity in cambodia-laos border |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04121-w https://doaj.org/article/6710227f026d4dc1a3f0419a43fbcddd |
long_lat |
ENVELOPE(7.559,7.559,62.581,62.581) |
geographic |
Arctic Indian Siem |
geographic_facet |
Arctic Indian Siem |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-12 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04121-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04121-w 1475-2875 https://doaj.org/article/6710227f026d4dc1a3f0419a43fbcddd |
op_doi |
https://doi.org/10.1186/s12936-022-04121-w |
container_title |
Malaria Journal |
container_volume |
21 |
container_issue |
1 |
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1766347036947382272 |