High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria.
This study examined pyrethroid resistance intensity and mechanisms in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West Nigeria. Resistance statuses to permethrin, lambda-cyhalothrin and alphacypermethrin were determined with both WHO and CDC resistance bioassays....
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0010525 https://doaj.org/article/66d24d2655934088bd84ff0a373d16ef |
id |
ftdoajarticles:oai:doaj.org/article:66d24d2655934088bd84ff0a373d16ef |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:66d24d2655934088bd84ff0a373d16ef 2023-05-15T15:14:47+02:00 High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. Ahmed Idowu Omotayo Musa Mustapha Dogara Danjuma Sufi Tasiu Shuaibu Joshua Balogun Salwa Dawaki Bature Muktar Kamoru Adeniyi Nura Garba Isah Namadi Hafiz Abdullahi Adam Shuaibu Adamu Hamza Abdullahi Abubakar Sulaiman Adedayo Olatunbosun Oduola 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010525 https://doaj.org/article/66d24d2655934088bd84ff0a373d16ef EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010525 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010525 https://doaj.org/article/66d24d2655934088bd84ff0a373d16ef PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010525 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010525 2022-12-30T23:19:51Z This study examined pyrethroid resistance intensity and mechanisms in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West Nigeria. Resistance statuses to permethrin, lambda-cyhalothrin and alphacypermethrin were determined with both WHO and CDC resistance bioassays. Synergist assay was conducted by pre-exposing the populations to Piperonyl butoxide (PBO) using the WHO method. Resistance intensities to 2x, 5x and 10x of diagnostic concentrations were determined with the CDC bottle method. Species analysis and presence of knockdown mutation (Leu-Phe) were done using Polymerase Chain Reaction (PCR). Results showed that Cx. quinquefasciatus was the only Culex spp. present and "Kdr-west" mutation was not detected in all analyzed samples. Using WHO method, Cx. quinquefasciatus resistance to permethrin was detected in Dutse (12.2%) and Kafin-Hausa (77.78%). Lambda-cyhalothrin resistance was recorded only in Kafin-Hausa (83.95%) with resistance suspected in Ringim (90%). Resistance to alphacypermethrin was recorded in all locations. Pre-exposure to PBO led to 100% mortality to alphacypermethrin and lambda-cyhalothrin in Ringim while mortality to permethrin and alphacypermethrin in Dutse increased from 12.2% to 97.5% and 64.37% to 79.52% respectively. Using CDC bottle bioassay, resistance was also recorded in all populations and the result shows a significant positive correlation (R2 = 0.728, p = 0.026) with the result from the WHO bioassay. Results of resistance intensity revealed a very high level of resistance in Kafin-Hausa with susceptibility to lambda-cyhalothrin and alphacypermethrin not achieved at 10x of diagnostic doses. Resistance intensity was also high in Dutse with susceptibility to all insecticides not achieved at 5x of diagnostic doses. Widespread and high intensity of resistance in Cx. quinquefasciatus from North-West Nigeria is a major threat to the control of diseases transmitted by Culex and other mosquito species. It is a challenge that needs to be adequately ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Lambda ENVELOPE(-62.983,-62.983,-64.300,-64.300) PLOS Neglected Tropical Diseases 16 6 e0010525 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ahmed Idowu Omotayo Musa Mustapha Dogara Danjuma Sufi Tasiu Shuaibu Joshua Balogun Salwa Dawaki Bature Muktar Kamoru Adeniyi Nura Garba Isah Namadi Hafiz Abdullahi Adam Shuaibu Adamu Hamza Abdullahi Abubakar Sulaiman Adedayo Olatunbosun Oduola High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
This study examined pyrethroid resistance intensity and mechanisms in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West Nigeria. Resistance statuses to permethrin, lambda-cyhalothrin and alphacypermethrin were determined with both WHO and CDC resistance bioassays. Synergist assay was conducted by pre-exposing the populations to Piperonyl butoxide (PBO) using the WHO method. Resistance intensities to 2x, 5x and 10x of diagnostic concentrations were determined with the CDC bottle method. Species analysis and presence of knockdown mutation (Leu-Phe) were done using Polymerase Chain Reaction (PCR). Results showed that Cx. quinquefasciatus was the only Culex spp. present and "Kdr-west" mutation was not detected in all analyzed samples. Using WHO method, Cx. quinquefasciatus resistance to permethrin was detected in Dutse (12.2%) and Kafin-Hausa (77.78%). Lambda-cyhalothrin resistance was recorded only in Kafin-Hausa (83.95%) with resistance suspected in Ringim (90%). Resistance to alphacypermethrin was recorded in all locations. Pre-exposure to PBO led to 100% mortality to alphacypermethrin and lambda-cyhalothrin in Ringim while mortality to permethrin and alphacypermethrin in Dutse increased from 12.2% to 97.5% and 64.37% to 79.52% respectively. Using CDC bottle bioassay, resistance was also recorded in all populations and the result shows a significant positive correlation (R2 = 0.728, p = 0.026) with the result from the WHO bioassay. Results of resistance intensity revealed a very high level of resistance in Kafin-Hausa with susceptibility to lambda-cyhalothrin and alphacypermethrin not achieved at 10x of diagnostic doses. Resistance intensity was also high in Dutse with susceptibility to all insecticides not achieved at 5x of diagnostic doses. Widespread and high intensity of resistance in Cx. quinquefasciatus from North-West Nigeria is a major threat to the control of diseases transmitted by Culex and other mosquito species. It is a challenge that needs to be adequately ... |
format |
Article in Journal/Newspaper |
author |
Ahmed Idowu Omotayo Musa Mustapha Dogara Danjuma Sufi Tasiu Shuaibu Joshua Balogun Salwa Dawaki Bature Muktar Kamoru Adeniyi Nura Garba Isah Namadi Hafiz Abdullahi Adam Shuaibu Adamu Hamza Abdullahi Abubakar Sulaiman Adedayo Olatunbosun Oduola |
author_facet |
Ahmed Idowu Omotayo Musa Mustapha Dogara Danjuma Sufi Tasiu Shuaibu Joshua Balogun Salwa Dawaki Bature Muktar Kamoru Adeniyi Nura Garba Isah Namadi Hafiz Abdullahi Adam Shuaibu Adamu Hamza Abdullahi Abubakar Sulaiman Adedayo Olatunbosun Oduola |
author_sort |
Ahmed Idowu Omotayo |
title |
High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. |
title_short |
High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. |
title_full |
High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. |
title_fullStr |
High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. |
title_full_unstemmed |
High pyrethroid-resistance intensity in Culex quinquefasciatus (Say) (Diptera: Culicidae) populations from Jigawa, North-West, Nigeria. |
title_sort |
high pyrethroid-resistance intensity in culex quinquefasciatus (say) (diptera: culicidae) populations from jigawa, north-west, nigeria. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010525 https://doaj.org/article/66d24d2655934088bd84ff0a373d16ef |
long_lat |
ENVELOPE(-62.983,-62.983,-64.300,-64.300) |
geographic |
Arctic Lambda |
geographic_facet |
Arctic Lambda |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010525 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010525 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010525 https://doaj.org/article/66d24d2655934088bd84ff0a373d16ef |
op_doi |
https://doi.org/10.1371/journal.pntd.0010525 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
6 |
container_start_page |
e0010525 |
_version_ |
1766345193471082496 |