Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection.
Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strain...
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ftdoajarticles:oai:doaj.org/article:66a0d4683a054ffaa295416b0b29d66e 2023-05-15T15:04:29+02:00 Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. Ryuta Uraki Andrew K Hastings Andrea Gloria-Soria Jeffrey R Powell Erol Fikrig 2018-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006350 https://doaj.org/article/66a0d4683a054ffaa295416b0b29d66e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5854422?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006350 https://doaj.org/article/66a0d4683a054ffaa295416b0b29d66e PLoS Neglected Tropical Diseases, Vol 12, Iss 3, p e0006350 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006350 2023-01-08T01:25:37Z Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 3 e0006350 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ryuta Uraki Andrew K Hastings Andrea Gloria-Soria Jeffrey R Powell Erol Fikrig Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics. |
format |
Article in Journal/Newspaper |
author |
Ryuta Uraki Andrew K Hastings Andrea Gloria-Soria Jeffrey R Powell Erol Fikrig |
author_facet |
Ryuta Uraki Andrew K Hastings Andrea Gloria-Soria Jeffrey R Powell Erol Fikrig |
author_sort |
Ryuta Uraki |
title |
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. |
title_short |
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. |
title_full |
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. |
title_fullStr |
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. |
title_full_unstemmed |
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. |
title_sort |
altered vector competence in an experimental mosquito-mouse transmission model of zika infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006350 https://doaj.org/article/66a0d4683a054ffaa295416b0b29d66e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 3, p e0006350 (2018) |
op_relation |
http://europepmc.org/articles/PMC5854422?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006350 https://doaj.org/article/66a0d4683a054ffaa295416b0b29d66e |
op_doi |
https://doi.org/10.1371/journal.pntd.0006350 |
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PLOS Neglected Tropical Diseases |
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12 |
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3 |
container_start_page |
e0006350 |
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1766336252007677952 |