Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei
The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii , a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared...
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ftdoajarticles:oai:doaj.org/article:6676a010da4f4d77b6254def8391f2f8 2024-01-07T09:40:03+01:00 Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei Alessia Ametrano Simona Picchietti Laura Guerra Stefano Giacomelli Umberto Oreste Maria Rosaria Coscia 2022-07-01T00:00:00Z https://doi.org/10.3390/ijms23147783 https://doaj.org/article/6676a010da4f4d77b6254def8391f2f8 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/14/7783 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23147783 1422-0067 1661-6596 https://doaj.org/article/6676a010da4f4d77b6254def8391f2f8 International Journal of Molecular Sciences, Vol 23, Iss 14, p 7783 (2022) pIgR gene structure cold environment gene expression teleost immunity adaptive evolution Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms23147783 2023-12-10T01:42:22Z The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii , a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to the pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and 1 non-Antarctic ( Cottoperca gobio ) notothenioid species, the latter living in the less-cold peri-Antarctic sea. Antarctic pIgR genes displayed particularly long introns marked by sites of transposable elements and transcription factors. Furthermore, analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to the Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold-adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold-adapted species, pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic International Journal of Molecular Sciences 23 14 7783 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
pIgR gene structure cold environment gene expression teleost immunity adaptive evolution Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
pIgR gene structure cold environment gene expression teleost immunity adaptive evolution Biology (General) QH301-705.5 Chemistry QD1-999 Alessia Ametrano Simona Picchietti Laura Guerra Stefano Giacomelli Umberto Oreste Maria Rosaria Coscia Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
topic_facet |
pIgR gene structure cold environment gene expression teleost immunity adaptive evolution Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii , a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to the pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and 1 non-Antarctic ( Cottoperca gobio ) notothenioid species, the latter living in the less-cold peri-Antarctic sea. Antarctic pIgR genes displayed particularly long introns marked by sites of transposable elements and transcription factors. Furthermore, analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to the Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold-adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold-adapted species, pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred. |
format |
Article in Journal/Newspaper |
author |
Alessia Ametrano Simona Picchietti Laura Guerra Stefano Giacomelli Umberto Oreste Maria Rosaria Coscia |
author_facet |
Alessia Ametrano Simona Picchietti Laura Guerra Stefano Giacomelli Umberto Oreste Maria Rosaria Coscia |
author_sort |
Alessia Ametrano |
title |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_short |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_full |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_fullStr |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_full_unstemmed |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_sort |
comparative analysis of the pigr gene from the antarctic teleost trematomus bernacchii reveals distinctive features of cold-adapted notothenioidei |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23147783 https://doaj.org/article/6676a010da4f4d77b6254def8391f2f8 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 14, p 7783 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/14/7783 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23147783 1422-0067 1661-6596 https://doaj.org/article/6676a010da4f4d77b6254def8391f2f8 |
op_doi |
https://doi.org/10.3390/ijms23147783 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
14 |
container_start_page |
7783 |
_version_ |
1787430410871898112 |