Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
Serratia marcescens is a species that belongs to the family of Yersiniaceae. This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3R) of the species Serratia marcesc...
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ftdoajarticles:oai:doaj.org/article:662737dfebc945bda878171b8cef8479 2023-05-15T16:31:36+02:00 Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) Ines Friedrich Bernhard Bodenberger Hannes Neubauer Robert Hertel Rolf Daniel 2021-01-01T00:00:00Z https://doaj.org/article/662737dfebc945bda878171b8cef8479 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575298/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/662737dfebc945bda878171b8cef8479 PLoS ONE, Vol 16, Iss 11 (2021) Medicine R Science Q article 2021 ftdoajarticles 2022-12-31T09:17:14Z Serratia marcescens is a species that belongs to the family of Yersiniaceae. This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3R) of the species Serratia marcescens. The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3R belongs to the species Serratia marcescens. In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S. marcescens LVF3R is a potential PGPR strain. It harbors genes coding for indole acetic acid (IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. Article in Journal/Newspaper Groenlandia Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Ines Friedrich Bernhard Bodenberger Hannes Neubauer Robert Hertel Rolf Daniel Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
topic_facet |
Medicine R Science Q |
description |
Serratia marcescens is a species that belongs to the family of Yersiniaceae. This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3R) of the species Serratia marcescens. The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3R belongs to the species Serratia marcescens. In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S. marcescens LVF3R is a potential PGPR strain. It harbors genes coding for indole acetic acid (IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. |
format |
Article in Journal/Newspaper |
author |
Ines Friedrich Bernhard Bodenberger Hannes Neubauer Robert Hertel Rolf Daniel |
author_facet |
Ines Friedrich Bernhard Bodenberger Hannes Neubauer Robert Hertel Rolf Daniel |
author_sort |
Ines Friedrich |
title |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_short |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_full |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_fullStr |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_full_unstemmed |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_sort |
down in the pond: isolation and characterization of a new serratia marcescens strain (lvf3) from the surface water near frog’s lettuce (groenlandia densa) |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/662737dfebc945bda878171b8cef8479 |
genre |
Groenlandia |
genre_facet |
Groenlandia |
op_source |
PLoS ONE, Vol 16, Iss 11 (2021) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575298/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/662737dfebc945bda878171b8cef8479 |
_version_ |
1766021346861514752 |