Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic
The enzymatic electron transport system (ETS) assay is frequently used as a proxy of respiratory activity in planktonic communities. It is thought to estimate the maximum overall activity of the enzymes associated with the respiratory ETS systems in both eukaryotic and prokaryotic organisms. Thus, i...
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ftdoajarticles:oai:doaj.org/article:661f048e16954564b9e1bc46e81768a3 2023-05-15T17:36:55+02:00 Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic Alba Filella Isabel Baños María F. Montero Nauzet Hernández-Hernández Adriana Rodríguez-Santos Andrea Ludwig Ulf Riebesell Javier Arístegui 2018-08-01T00:00:00Z https://doi.org/10.3389/fmars.2018.00310 https://doaj.org/article/661f048e16954564b9e1bc46e81768a3 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmars.2018.00310/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2018.00310 https://doaj.org/article/661f048e16954564b9e1bc46e81768a3 Frontiers in Marine Science, Vol 5 (2018) plankton respiration ETS activity R/ETS ocean acidification nutrient fertilization mesocosm Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2018 ftdoajarticles https://doi.org/10.3389/fmars.2018.00310 2022-12-31T12:23:48Z The enzymatic electron transport system (ETS) assay is frequently used as a proxy of respiratory activity in planktonic communities. It is thought to estimate the maximum overall activity of the enzymes associated with the respiratory ETS systems in both eukaryotic and prokaryotic organisms. Thus, in order to derive actual respiration rates (R) from ETS it is necessary to determine empirical R/ETS conversion algorithms. In this study we explore the temporal development of R and ETS activity in natural plankton communities (from bacteria to large phytoplankton) enclosed in mesocosms, treated with different CO2 concentrations. The experiment lasted 30 days, during which abrupt changes in community structure and biomass occurred through a sharp transition from oligotrophy (phase I) to highly eutrophic conditions (phase II) after nutrient-induced fertilization (day 18). R and ETS did not show any response to CO2 under oligotrophic conditions, but R increased significantly more in the two high CO2 mesocosms after fertilization, coinciding with a sharp rise in large phytoplankton (mostly diatoms). R and ETS were significantly correlated only during the eutrophic phase. The R/ETS ranged more than threefold in magnitude during the experiment, with phase-averaged values significantly higher under oligotrophic conditions (0.7–1.1) than after nutrient fertilization (0.5–0.7). We did not find any significant relationship between R/ETS and community structure or biomass, although R correlated significantly with total biomass after fertilization in the four mesocosms. Multiple stepwise regression models show that large phytoplankton explains most of the variance in R during phases I (86%) and II (53%) and of ETS (86%) during phase II, while picophytoplankton contributes up to 73% to explain the variance in the ETS model during phase I. Our results suggest that R/ETS may be too variable in the ocean as to apply constant values to different communities living under contrasting environmental conditions. Controlled experiments ... Article in Journal/Newspaper North Atlantic Ocean acidification Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 5 |
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language |
English |
topic |
plankton respiration ETS activity R/ETS ocean acidification nutrient fertilization mesocosm Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
plankton respiration ETS activity R/ETS ocean acidification nutrient fertilization mesocosm Science Q General. Including nature conservation geographical distribution QH1-199.5 Alba Filella Isabel Baños María F. Montero Nauzet Hernández-Hernández Adriana Rodríguez-Santos Andrea Ludwig Ulf Riebesell Javier Arístegui Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
topic_facet |
plankton respiration ETS activity R/ETS ocean acidification nutrient fertilization mesocosm Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The enzymatic electron transport system (ETS) assay is frequently used as a proxy of respiratory activity in planktonic communities. It is thought to estimate the maximum overall activity of the enzymes associated with the respiratory ETS systems in both eukaryotic and prokaryotic organisms. Thus, in order to derive actual respiration rates (R) from ETS it is necessary to determine empirical R/ETS conversion algorithms. In this study we explore the temporal development of R and ETS activity in natural plankton communities (from bacteria to large phytoplankton) enclosed in mesocosms, treated with different CO2 concentrations. The experiment lasted 30 days, during which abrupt changes in community structure and biomass occurred through a sharp transition from oligotrophy (phase I) to highly eutrophic conditions (phase II) after nutrient-induced fertilization (day 18). R and ETS did not show any response to CO2 under oligotrophic conditions, but R increased significantly more in the two high CO2 mesocosms after fertilization, coinciding with a sharp rise in large phytoplankton (mostly diatoms). R and ETS were significantly correlated only during the eutrophic phase. The R/ETS ranged more than threefold in magnitude during the experiment, with phase-averaged values significantly higher under oligotrophic conditions (0.7–1.1) than after nutrient fertilization (0.5–0.7). We did not find any significant relationship between R/ETS and community structure or biomass, although R correlated significantly with total biomass after fertilization in the four mesocosms. Multiple stepwise regression models show that large phytoplankton explains most of the variance in R during phases I (86%) and II (53%) and of ETS (86%) during phase II, while picophytoplankton contributes up to 73% to explain the variance in the ETS model during phase I. Our results suggest that R/ETS may be too variable in the ocean as to apply constant values to different communities living under contrasting environmental conditions. Controlled experiments ... |
format |
Article in Journal/Newspaper |
author |
Alba Filella Isabel Baños María F. Montero Nauzet Hernández-Hernández Adriana Rodríguez-Santos Andrea Ludwig Ulf Riebesell Javier Arístegui |
author_facet |
Alba Filella Isabel Baños María F. Montero Nauzet Hernández-Hernández Adriana Rodríguez-Santos Andrea Ludwig Ulf Riebesell Javier Arístegui |
author_sort |
Alba Filella |
title |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_short |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_full |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_fullStr |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_full_unstemmed |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_sort |
plankton community respiration and ets activity under variable co2 and nutrient fertilization during a mesocosm study in the subtropical north atlantic |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmars.2018.00310 https://doaj.org/article/661f048e16954564b9e1bc46e81768a3 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_source |
Frontiers in Marine Science, Vol 5 (2018) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmars.2018.00310/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2018.00310 https://doaj.org/article/661f048e16954564b9e1bc46e81768a3 |
op_doi |
https://doi.org/10.3389/fmars.2018.00310 |
container_title |
Frontiers in Marine Science |
container_volume |
5 |
_version_ |
1766136567309533184 |