Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model.
Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a major cause of sepsis and mortality in endemic regions of Southeast Asia and Northern Australia. B. pseudomallei is a potential bioterrorism agent due to its high infectivity, especially via inhalation, and its inhere...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0009125 https://doaj.org/article/65e590f676164defb6324dee7d116e6d |
id |
ftdoajarticles:oai:doaj.org/article:65e590f676164defb6324dee7d116e6d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:65e590f676164defb6324dee7d116e6d 2023-05-15T15:15:13+02:00 Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. Sylvia R Trevino Jennifer L Dankmeyer David P Fetterer Christopher P Klimko Jo Lynne W Raymond Alicia M Moreau Carl Soffler David M Waag Patricia L Worsham Kei Amemiya Sara I Ruiz Christopher K Cote Teresa Krakauer 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009125 https://doaj.org/article/65e590f676164defb6324dee7d116e6d EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009125 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009125 https://doaj.org/article/65e590f676164defb6324dee7d116e6d PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009125 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009125 2022-12-31T08:00:29Z Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a major cause of sepsis and mortality in endemic regions of Southeast Asia and Northern Australia. B. pseudomallei is a potential bioterrorism agent due to its high infectivity, especially via inhalation, and its inherent resistance to antimicrobials. There is currently no vaccine for melioidosis and antibiotic treatment can fail due to innate drug resistance, delayed diagnosis and treatment, or insufficient duration of treatment. A well-characterized animal model that mimics human melioidosis is needed for the development of new medical countermeasures. This study first characterized the disease progression of melioidosis in the African green monkey (AGM) and rhesus macaque (RM) for non-human primate model down-selection. All AGMs developed acute lethal disease similar to that described in human acute infection following exposure to aerosolized B. pseudomallei strain HBPUB10134a. Only 20% of RMs succumbed to acute disease. Disease progression, immune response and pathology of two other strains of B. pseudomallei, K96243 and MSHR5855, were also compared using AGMs. These three B. pseudomallei strains represent a highly virulent strain from Thailand (HBPUB101034a), a highly virulent strains from Australia (MSHR5855), and a commonly used laboratory strains originating from Thailand (K96243). Animals were observed for clinical signs of infection and blood samples were analyzed for cytokine responses, blood chemistry and leukocyte changes in order to characterize bacterial infection. AGMs experienced fever after exposure to aerosolized B. pseudomallei at the onset of acute disease. Inflammation, abscesses and/or pyogranulomas were observed in lung with all three strains of B. pseudomallei. Inflammation, abscesses and/or pyogranulomas were observed in lymph nodes, spleen, liver and/or kidney with B. pseudomallei, HBPUB10134a and K96243. Additionally, the Australian strain MSHR5855 induced brain lesions in one AGM similar to clinical ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0009125 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sylvia R Trevino Jennifer L Dankmeyer David P Fetterer Christopher P Klimko Jo Lynne W Raymond Alicia M Moreau Carl Soffler David M Waag Patricia L Worsham Kei Amemiya Sara I Ruiz Christopher K Cote Teresa Krakauer Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a major cause of sepsis and mortality in endemic regions of Southeast Asia and Northern Australia. B. pseudomallei is a potential bioterrorism agent due to its high infectivity, especially via inhalation, and its inherent resistance to antimicrobials. There is currently no vaccine for melioidosis and antibiotic treatment can fail due to innate drug resistance, delayed diagnosis and treatment, or insufficient duration of treatment. A well-characterized animal model that mimics human melioidosis is needed for the development of new medical countermeasures. This study first characterized the disease progression of melioidosis in the African green monkey (AGM) and rhesus macaque (RM) for non-human primate model down-selection. All AGMs developed acute lethal disease similar to that described in human acute infection following exposure to aerosolized B. pseudomallei strain HBPUB10134a. Only 20% of RMs succumbed to acute disease. Disease progression, immune response and pathology of two other strains of B. pseudomallei, K96243 and MSHR5855, were also compared using AGMs. These three B. pseudomallei strains represent a highly virulent strain from Thailand (HBPUB101034a), a highly virulent strains from Australia (MSHR5855), and a commonly used laboratory strains originating from Thailand (K96243). Animals were observed for clinical signs of infection and blood samples were analyzed for cytokine responses, blood chemistry and leukocyte changes in order to characterize bacterial infection. AGMs experienced fever after exposure to aerosolized B. pseudomallei at the onset of acute disease. Inflammation, abscesses and/or pyogranulomas were observed in lung with all three strains of B. pseudomallei. Inflammation, abscesses and/or pyogranulomas were observed in lymph nodes, spleen, liver and/or kidney with B. pseudomallei, HBPUB10134a and K96243. Additionally, the Australian strain MSHR5855 induced brain lesions in one AGM similar to clinical ... |
format |
Article in Journal/Newspaper |
author |
Sylvia R Trevino Jennifer L Dankmeyer David P Fetterer Christopher P Klimko Jo Lynne W Raymond Alicia M Moreau Carl Soffler David M Waag Patricia L Worsham Kei Amemiya Sara I Ruiz Christopher K Cote Teresa Krakauer |
author_facet |
Sylvia R Trevino Jennifer L Dankmeyer David P Fetterer Christopher P Klimko Jo Lynne W Raymond Alicia M Moreau Carl Soffler David M Waag Patricia L Worsham Kei Amemiya Sara I Ruiz Christopher K Cote Teresa Krakauer |
author_sort |
Sylvia R Trevino |
title |
Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. |
title_short |
Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. |
title_full |
Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. |
title_fullStr |
Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. |
title_full_unstemmed |
Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model. |
title_sort |
comparative virulence of three different strains of burkholderia pseudomallei in an aerosol non-human primate model. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009125 https://doaj.org/article/65e590f676164defb6324dee7d116e6d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009125 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009125 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009125 https://doaj.org/article/65e590f676164defb6324dee7d116e6d |
op_doi |
https://doi.org/10.1371/journal.pntd.0009125 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
2 |
container_start_page |
e0009125 |
_version_ |
1766345596992487424 |