Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising pr...
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Online Access: | https://doi.org/10.3390/microorganisms9112389 https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1 |
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ftdoajarticles:oai:doaj.org/article:65b07f0aaf55470a9e56cb4e3c01e8b1 2023-05-15T17:31:09+02:00 Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha 2021-11-01T00:00:00Z https://doi.org/10.3390/microorganisms9112389 https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1 EN eng MDPI AG https://www.mdpi.com/2076-2607/9/11/2389 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9112389 2076-2607 https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1 Microorganisms, Vol 9, Iss 2389, p 2389 (2021) microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9112389 2022-12-31T08:03:59Z Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Mid-Atlantic Ridge Microorganisms 9 11 2389 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5 |
spellingShingle |
microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5 Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) |
topic_facet |
microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5 |
description |
Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater. |
format |
Article in Journal/Newspaper |
author |
Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha |
author_facet |
Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha |
author_sort |
Maria Paola Tomasino |
title |
Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) |
title_short |
Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) |
title_full |
Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) |
title_fullStr |
Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) |
title_full_unstemmed |
Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean) |
title_sort |
diversity and hydrocarbon-degrading potential of deep-sea microbial community from the mid-atlantic ridge, south of the azores (north atlantic ocean) |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9112389 https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1 |
geographic |
Mid-Atlantic Ridge |
geographic_facet |
Mid-Atlantic Ridge |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Microorganisms, Vol 9, Iss 2389, p 2389 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/11/2389 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9112389 2076-2607 https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1 |
op_doi |
https://doi.org/10.3390/microorganisms9112389 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
11 |
container_start_page |
2389 |
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1766128492054839296 |