Optimization of a Nanofiltration Desalination Process for Antarctic Krill Peptides Using Orthogonal Tests

• Main Authors: Fuhou LI, Yuelei HUANG, Xiaofang LIU, Kailiang LENG, Lingzhao WANG, Yuan YU, Junkui MIAO
• Format: Article in Journal/Newspaper
• Language: English; Chinese
• Published: Science Press, PR China 2023
• Subjects: antarctic krill (euphausia superba); peptides; orthogonal test; nanofiltration; desalination; Aquaculture. Fisheries. Angling; SH1-691; Antarctic; Southern Ocean; Antarc*; Antarctic Krill; Euphausia superba
• Online Access: https://doi.org/10.19663/j.issn2095-9869.20210901002; https://doaj.org/article/658e3a1ab64c42eb9d67d827ad3d6eb4

Antarctic krill (Euphausia superba), an important group of marine zooplankton in the Southern Ocean, is the only fishery resource with extremely rich reserves and a low degree of development in the world. Antarctic krill is considered to be the greatest potential source of high-quality marine protein resources due to its abundant biomass and high protein content. Peptides prepared from Antarctic krill exhibit multiple physiological activities, including osteoporosis relief, glucose metabolism regulation, blood pressure amelioration, antioxidation, fatigue alleviation, and anti-aging activity. The production and development of Antarctic krill peptides has recently become an industry focus; however, existing research has been limited to the optimization of enzymatic hydrolysis processes, mainly involving the screening of suitable enzymes and the optimization of enzymatic hydrolysis conditions. Due to the high mineral content of Antarctic krill and the introduction of buffer salt in the process of enzymatic hydrolysis, current Antarctic krill peptides products have a high salt content, which leads to poor sensory experience and health risks. Hence, a process for desalination of Antarctic krill peptides is needed. Desalination methods for bioactive substances include dialysis, ultrafiltration, nanofiltration, electrodialysis, and macroporous resin adsorption. In the field of membrane separation, nanofiltration technology has been widely used in the purification, concentration, and desalination of food components owing to its advantages: low operation cost, no introduction of exogenous substances, no destruction of materials, and low rejection rate of monovalent ions. In order to improve product quality and ensure market expansion, the process of desalination of Antarctic krill peptides using nanofiltration technology was studied and optimized in this study.Defatted Antarctic krill powder was enzymatically hydrolyzed by alkaline protease to obtain Antarctic krill peptides for further use. The main factors affecting ...