Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect
Abstract Background: Members of the genus Cupiennius Simon, 1891 are categorized as wandering spiders and are part of the family Trechaleidae. The genomics and proteomics of Cupiennius spiders from North America remain uncharacterized. The present study explores for the first time molecular data fro...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2023-0098 https://doaj.org/article/655f2c48bb01449aa420124e30cc5ba7 |
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ftdoajarticles:oai:doaj.org/article:655f2c48bb01449aa420124e30cc5ba7 2024-09-09T19:28:08+00:00 Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect Montserrat Padilla-Villavicencio Gerardo Corzo Karina Guillén-Navarro Guillermo Ibarra-Núñez Iván Arenas Fernando Zamudio Elia Diego-García 2024-08-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2023-0098 https://doaj.org/article/655f2c48bb01449aa420124e30cc5ba7 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992024000100311&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v30/1678-9199-jvatitd-30-e20230098.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2023-0098 https://doaj.org/article/655f2c48bb01449aa420124e30cc5ba7 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 30 (2024) Cupiennius Spider toxin Venom COI DNA barcoding Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2024 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2023-0098 2024-08-19T14:56:39Z Abstract Background: Members of the genus Cupiennius Simon, 1891 are categorized as wandering spiders and are part of the family Trechaleidae. The genomics and proteomics of Cupiennius spiders from North America remain uncharacterized. The present study explores for the first time molecular data from the endemic species Cupiennius chiapanensis Medina, 2006, and also presents new data for Cupiennius salei (Keyserling, 1878), both collected in southern Mexico. Methods: In total, 88 Cupiennius specimens were collected from southern Mexico and morphologically identified. DNA was extracted and the mitochondrial COI fragment was amplified. COI sequences were analyzed, and a phylogenetic tree was inferred for species from the Americas. Genetic diversity was analyzed using haplotype networks and gene distances. Venom was obtained from C. chiapanensis and C. salei by electrostimulation. The venom was separated by HPLC, visualized using SDS-PAGE, and quantified for use in toxicity bioassays in mice and insects. Results: Analysis of COI sequences from C. chiapanensis showed 94% identity with C. salei, while C. salei exhibited 94-97% identity with sequences from Central and South American conspecifics. The venom from C. chiapanensis exhibited toxic activity against crickets. Venoms from C. chiapanensis and C. salei caused death in Anastrepha obliqua flies. Analysis of venom fractions from C. salei and C. chiapanensis revealed molecular masses of a similar size as some previously reported toxins and neurotoxic components. We determined the amino acid sequences of ChiaTx1 and ChiaTx2, toxins that are reported here for the first time and which showed toxicity against mice and insects. Conclusion: Our work is the first to report COI-based DNA barcoding sequences from southern Mexican Cupiennius spiders. Compounds with toxic activity were identified in venom from both species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Medina ENVELOPE(-66.233,-66.233,-68.453,-68.453) Journal of Venomous Animals and Toxins including Tropical Diseases 30 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cupiennius Spider toxin Venom COI DNA barcoding Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Cupiennius Spider toxin Venom COI DNA barcoding Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Montserrat Padilla-Villavicencio Gerardo Corzo Karina Guillén-Navarro Guillermo Ibarra-Núñez Iván Arenas Fernando Zamudio Elia Diego-García Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect |
topic_facet |
Cupiennius Spider toxin Venom COI DNA barcoding Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Members of the genus Cupiennius Simon, 1891 are categorized as wandering spiders and are part of the family Trechaleidae. The genomics and proteomics of Cupiennius spiders from North America remain uncharacterized. The present study explores for the first time molecular data from the endemic species Cupiennius chiapanensis Medina, 2006, and also presents new data for Cupiennius salei (Keyserling, 1878), both collected in southern Mexico. Methods: In total, 88 Cupiennius specimens were collected from southern Mexico and morphologically identified. DNA was extracted and the mitochondrial COI fragment was amplified. COI sequences were analyzed, and a phylogenetic tree was inferred for species from the Americas. Genetic diversity was analyzed using haplotype networks and gene distances. Venom was obtained from C. chiapanensis and C. salei by electrostimulation. The venom was separated by HPLC, visualized using SDS-PAGE, and quantified for use in toxicity bioassays in mice and insects. Results: Analysis of COI sequences from C. chiapanensis showed 94% identity with C. salei, while C. salei exhibited 94-97% identity with sequences from Central and South American conspecifics. The venom from C. chiapanensis exhibited toxic activity against crickets. Venoms from C. chiapanensis and C. salei caused death in Anastrepha obliqua flies. Analysis of venom fractions from C. salei and C. chiapanensis revealed molecular masses of a similar size as some previously reported toxins and neurotoxic components. We determined the amino acid sequences of ChiaTx1 and ChiaTx2, toxins that are reported here for the first time and which showed toxicity against mice and insects. Conclusion: Our work is the first to report COI-based DNA barcoding sequences from southern Mexican Cupiennius spiders. Compounds with toxic activity were identified in venom from both species. |
format |
Article in Journal/Newspaper |
author |
Montserrat Padilla-Villavicencio Gerardo Corzo Karina Guillén-Navarro Guillermo Ibarra-Núñez Iván Arenas Fernando Zamudio Elia Diego-García |
author_facet |
Montserrat Padilla-Villavicencio Gerardo Corzo Karina Guillén-Navarro Guillermo Ibarra-Núñez Iván Arenas Fernando Zamudio Elia Diego-García |
author_sort |
Montserrat Padilla-Villavicencio |
title |
Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect |
title_short |
Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect |
title_full |
Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect |
title_fullStr |
Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect |
title_full_unstemmed |
Cupiennius spiders (Trechaleidae) from southern Mexico: DNA barcoding, venomics, and biological effect |
title_sort |
cupiennius spiders (trechaleidae) from southern mexico: dna barcoding, venomics, and biological effect |
publisher |
SciELO |
publishDate |
2024 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2023-0098 https://doaj.org/article/655f2c48bb01449aa420124e30cc5ba7 |
long_lat |
ENVELOPE(-66.233,-66.233,-68.453,-68.453) |
geographic |
Arctic Medina |
geographic_facet |
Arctic Medina |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 30 (2024) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992024000100311&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v30/1678-9199-jvatitd-30-e20230098.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2023-0098 https://doaj.org/article/655f2c48bb01449aa420124e30cc5ba7 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2023-0098 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
30 |
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1809897414243385344 |