Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm
The research aimed to determine the effect of different taurine concentrations on the duration of sperm fertility preservation and the results of further use during cryopreservation. The taurine was injected into the sperm of the Siberian sturgeon by various methods under conditions of low positive...
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EDP Sciences
2021
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Online Access: | https://doi.org/10.1051/e3sconf/202127303010 https://doaj.org/article/653fa3e0f1b54f7ca8f623220bdd562f |
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ftdoajarticles:oai:doaj.org/article:653fa3e0f1b54f7ca8f623220bdd562f 2023-05-15T18:19:27+02:00 Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm Kovalev Konstantin Dokina Olga Pronina Natalia Balashov Dmitry Krasilnikova Aleksandra 2021-01-01T00:00:00Z https://doi.org/10.1051/e3sconf/202127303010 https://doaj.org/article/653fa3e0f1b54f7ca8f623220bdd562f EN FR eng fre EDP Sciences https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/49/e3sconf_interagromash2021_03010.pdf https://doaj.org/toc/2267-1242 2267-1242 doi:10.1051/e3sconf/202127303010 https://doaj.org/article/653fa3e0f1b54f7ca8f623220bdd562f E3S Web of Conferences, Vol 273, p 03010 (2021) Environmental sciences GE1-350 article 2021 ftdoajarticles https://doi.org/10.1051/e3sconf/202127303010 2022-12-31T06:49:13Z The research aimed to determine the effect of different taurine concentrations on the duration of sperm fertility preservation and the results of further use during cryopreservation. The taurine was injected into the sperm of the Siberian sturgeon by various methods under conditions of low positive temperatures. Immediately after preparation, the taurine was injected into native sperm at concentrations of 0.01, 0.05, and 0.1 mmol/ml. Two methods of introducing taurine into native sperm were tested: 1 (dry method) - adding taurine powder directly to native sperm and 2 (wet method) - adding taurine dissolved in saline to native sperm. The studies have shown that injections of taurine in an amount of 0.05 - 0.1 mmol/ml both dry and wet can be recommended as the method of long-term preservation of the native sperm of Siberian sturgeon viability. While storing Siberian sturgeon sperm for further cryopreservation the optimal taurine concentration is 0.01 mmol/ml. Article in Journal/Newspaper Siberian sturgeon Directory of Open Access Journals: DOAJ Articles E3S Web of Conferences 273 03010 |
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Directory of Open Access Journals: DOAJ Articles |
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English French |
topic |
Environmental sciences GE1-350 |
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Environmental sciences GE1-350 Kovalev Konstantin Dokina Olga Pronina Natalia Balashov Dmitry Krasilnikova Aleksandra Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm |
topic_facet |
Environmental sciences GE1-350 |
description |
The research aimed to determine the effect of different taurine concentrations on the duration of sperm fertility preservation and the results of further use during cryopreservation. The taurine was injected into the sperm of the Siberian sturgeon by various methods under conditions of low positive temperatures. Immediately after preparation, the taurine was injected into native sperm at concentrations of 0.01, 0.05, and 0.1 mmol/ml. Two methods of introducing taurine into native sperm were tested: 1 (dry method) - adding taurine powder directly to native sperm and 2 (wet method) - adding taurine dissolved in saline to native sperm. The studies have shown that injections of taurine in an amount of 0.05 - 0.1 mmol/ml both dry and wet can be recommended as the method of long-term preservation of the native sperm of Siberian sturgeon viability. While storing Siberian sturgeon sperm for further cryopreservation the optimal taurine concentration is 0.01 mmol/ml. |
format |
Article in Journal/Newspaper |
author |
Kovalev Konstantin Dokina Olga Pronina Natalia Balashov Dmitry Krasilnikova Aleksandra |
author_facet |
Kovalev Konstantin Dokina Olga Pronina Natalia Balashov Dmitry Krasilnikova Aleksandra |
author_sort |
Kovalev Konstantin |
title |
Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm |
title_short |
Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm |
title_full |
Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm |
title_fullStr |
Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm |
title_full_unstemmed |
Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm |
title_sort |
use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of siberian sturgeon sperm |
publisher |
EDP Sciences |
publishDate |
2021 |
url |
https://doi.org/10.1051/e3sconf/202127303010 https://doaj.org/article/653fa3e0f1b54f7ca8f623220bdd562f |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_source |
E3S Web of Conferences, Vol 273, p 03010 (2021) |
op_relation |
https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/49/e3sconf_interagromash2021_03010.pdf https://doaj.org/toc/2267-1242 2267-1242 doi:10.1051/e3sconf/202127303010 https://doaj.org/article/653fa3e0f1b54f7ca8f623220bdd562f |
op_doi |
https://doi.org/10.1051/e3sconf/202127303010 |
container_title |
E3S Web of Conferences |
container_volume |
273 |
container_start_page |
03010 |
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1766196540063350784 |