Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population
Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyp...
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University of Belgrade, University of Novi Sad
2012
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Online Access: | https://doi.org/10.2298/ABS1201321F https://doaj.org/article/65294d4a3dd44f5a952fc9e1b44f53d6 |
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ftdoajarticles:oai:doaj.org/article:65294d4a3dd44f5a952fc9e1b44f53d6 2023-05-15T15:50:23+02:00 Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. 2012-01-01T00:00:00Z https://doi.org/10.2298/ABS1201321F https://doaj.org/article/65294d4a3dd44f5a952fc9e1b44f53d6 EN eng University of Belgrade, University of Novi Sad http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641201321F.pdf https://doaj.org/toc/0354-4664 https://doaj.org/toc/1821-4339 doi:10.2298/ABS1201321F 0354-4664 1821-4339 https://doaj.org/article/65294d4a3dd44f5a952fc9e1b44f53d6 Archives of Biological Sciences, Vol 64, Iss 1, Pp 321-335 (2012) Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay Biology (General) QH301-705.5 article 2012 ftdoajarticles https://doi.org/10.2298/ABS1201321F 2022-12-31T14:26:07Z Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyping of degraded DNA. We provide the first application of SNP genotyping in an Italian non-invasive genetic monitoring project of the wolf. We compared three different techniques for genotyping SNPs: pyrosequencing, SNaPshot® and TaqMan® Probe Assay in Real-Time PCR. We successively genotyped nine SNPs using the TaqMan Probe Assay in 51 Italian wolves, 57 domestic dogs, 15 wolf x dog hybrids and 313 wolf scats collected in the northern Apennines. The obtained results were used to estimate genetic variability and PCR error rates in SNP genotyping protocols compared to standard microsatellite analysis. We evaluated the cost, laboratory effort and reliability of these different markers and discuss the possible future use of VeraCode, SNPlex and Fluidigm EP1 system in wild population monitoring. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Archives of Biological Sciences 64 1 321 335 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay Biology (General) QH301-705.5 |
spellingShingle |
Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay Biology (General) QH301-705.5 Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
topic_facet |
Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay Biology (General) QH301-705.5 |
description |
Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyping of degraded DNA. We provide the first application of SNP genotyping in an Italian non-invasive genetic monitoring project of the wolf. We compared three different techniques for genotyping SNPs: pyrosequencing, SNaPshot® and TaqMan® Probe Assay in Real-Time PCR. We successively genotyped nine SNPs using the TaqMan Probe Assay in 51 Italian wolves, 57 domestic dogs, 15 wolf x dog hybrids and 313 wolf scats collected in the northern Apennines. The obtained results were used to estimate genetic variability and PCR error rates in SNP genotyping protocols compared to standard microsatellite analysis. We evaluated the cost, laboratory effort and reliability of these different markers and discuss the possible future use of VeraCode, SNPlex and Fluidigm EP1 system in wild population monitoring. |
format |
Article in Journal/Newspaper |
author |
Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. |
author_facet |
Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. |
author_sort |
Fabbri Elena |
title |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_short |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_full |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_fullStr |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_full_unstemmed |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_sort |
comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
publisher |
University of Belgrade, University of Novi Sad |
publishDate |
2012 |
url |
https://doi.org/10.2298/ABS1201321F https://doaj.org/article/65294d4a3dd44f5a952fc9e1b44f53d6 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Archives of Biological Sciences, Vol 64, Iss 1, Pp 321-335 (2012) |
op_relation |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641201321F.pdf https://doaj.org/toc/0354-4664 https://doaj.org/toc/1821-4339 doi:10.2298/ABS1201321F 0354-4664 1821-4339 https://doaj.org/article/65294d4a3dd44f5a952fc9e1b44f53d6 |
op_doi |
https://doi.org/10.2298/ABS1201321F |
container_title |
Archives of Biological Sciences |
container_volume |
64 |
container_issue |
1 |
container_start_page |
321 |
op_container_end_page |
335 |
_version_ |
1766385338271399936 |