Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida
Turbot aquaculture production is an important economic activity in several countries around the world; nonetheless, the incidence of diseases, such furunculosis, caused by the etiological agent A. salmonicida subsp. salmonicida , is responsible for important losses to this industry worldwide. Given...
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Online Access: | https://doi.org/10.3390/ijms241612944 https://doaj.org/article/651f8e62b5194fbab60e7a93bee6a89c |
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ftdoajarticles:oai:doaj.org/article:651f8e62b5194fbab60e7a93bee6a89c 2023-09-26T15:22:54+02:00 Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida Carlos Fajardo Paulo Santos Ricardo Passos Mariana Vaz Rita Azeredo Marina Machado Sergio Fernández-Boo Teresa Baptista Benjamin Costas 2023-08-01T00:00:00Z https://doi.org/10.3390/ijms241612944 https://doaj.org/article/651f8e62b5194fbab60e7a93bee6a89c EN eng MDPI AG https://www.mdpi.com/1422-0067/24/16/12944 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241612944 1422-0067 1661-6596 https://doaj.org/article/651f8e62b5194fbab60e7a93bee6a89c International Journal of Molecular Sciences, Vol 24, Iss 12944, p 12944 (2023) furunculosis gene expression haematological profile oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241612944 2023-08-27T00:35:30Z Turbot aquaculture production is an important economic activity in several countries around the world; nonetheless, the incidence of diseases, such furunculosis, caused by the etiological agent A. salmonicida subsp. salmonicida , is responsible for important losses to this industry worldwide. Given this perspective, this study aimed to evaluate early immune responses in turbot ( S. maximus L.) following infection with A. salmonicida subsp. salmonicida . For this, 72 fish were individually weighed and randomly distributed into 6 tanks in a circulating seawater system. For the bacterial challenge, half of the individuals (3 tanks with 36 individuals) were infected using a peritoneal injection with the bacterial suspension, while the other half of individuals were injected with PBS and kept as a control group. Several factors linked to the innate immune response were studied, including not only haematological (white blood cells, red blood cells, haematocrit, haemoglobin, mean corpuscular volume, mean cell haemoglobin, mean corpuscular haemoglobin concentration, neutrophils, monocytes, lymphocytes, thrombocytes) and oxidative stress parameters, but also the analyses of the expression of 13 key immune-related genes ( tnf-α , il-1β , il-8 , pparα-1 , acox1 , tgf-β1 , nf-kB p65 , srebp-1 , il-10 , c3 , cpt1a , pcna , il-22 ). No significant differences were recorded in blood or innate humoral parameters (lysozyme, anti-protease, peroxidase) at the selected sampling points. There was neither any evidence of significant changes in the activity levels of the oxidative stress indicators (catalase, glutathione S-transferase, lipid peroxidation, superoxide dismutase). In contrast, pro-inflammatory ( tnf-α , il-1β ), anti-inflammatory ( il-10 ), and innate immune-related genes ( c3 ) were up-regulated, while another gene linked with the lipid metabolism ( acox1 ) was down-regulated. The results showed new insights about early responses of turbot following infection with A. salmonicida subsp. salmonicida . Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 16 12944 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
furunculosis gene expression haematological profile oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
furunculosis gene expression haematological profile oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999 Carlos Fajardo Paulo Santos Ricardo Passos Mariana Vaz Rita Azeredo Marina Machado Sergio Fernández-Boo Teresa Baptista Benjamin Costas Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida |
topic_facet |
furunculosis gene expression haematological profile oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Turbot aquaculture production is an important economic activity in several countries around the world; nonetheless, the incidence of diseases, such furunculosis, caused by the etiological agent A. salmonicida subsp. salmonicida , is responsible for important losses to this industry worldwide. Given this perspective, this study aimed to evaluate early immune responses in turbot ( S. maximus L.) following infection with A. salmonicida subsp. salmonicida . For this, 72 fish were individually weighed and randomly distributed into 6 tanks in a circulating seawater system. For the bacterial challenge, half of the individuals (3 tanks with 36 individuals) were infected using a peritoneal injection with the bacterial suspension, while the other half of individuals were injected with PBS and kept as a control group. Several factors linked to the innate immune response were studied, including not only haematological (white blood cells, red blood cells, haematocrit, haemoglobin, mean corpuscular volume, mean cell haemoglobin, mean corpuscular haemoglobin concentration, neutrophils, monocytes, lymphocytes, thrombocytes) and oxidative stress parameters, but also the analyses of the expression of 13 key immune-related genes ( tnf-α , il-1β , il-8 , pparα-1 , acox1 , tgf-β1 , nf-kB p65 , srebp-1 , il-10 , c3 , cpt1a , pcna , il-22 ). No significant differences were recorded in blood or innate humoral parameters (lysozyme, anti-protease, peroxidase) at the selected sampling points. There was neither any evidence of significant changes in the activity levels of the oxidative stress indicators (catalase, glutathione S-transferase, lipid peroxidation, superoxide dismutase). In contrast, pro-inflammatory ( tnf-α , il-1β ), anti-inflammatory ( il-10 ), and innate immune-related genes ( c3 ) were up-regulated, while another gene linked with the lipid metabolism ( acox1 ) was down-regulated. The results showed new insights about early responses of turbot following infection with A. salmonicida subsp. salmonicida . |
format |
Article in Journal/Newspaper |
author |
Carlos Fajardo Paulo Santos Ricardo Passos Mariana Vaz Rita Azeredo Marina Machado Sergio Fernández-Boo Teresa Baptista Benjamin Costas |
author_facet |
Carlos Fajardo Paulo Santos Ricardo Passos Mariana Vaz Rita Azeredo Marina Machado Sergio Fernández-Boo Teresa Baptista Benjamin Costas |
author_sort |
Carlos Fajardo |
title |
Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida |
title_short |
Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida |
title_full |
Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida |
title_fullStr |
Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida |
title_full_unstemmed |
Early Molecular Immune Responses of Turbot ( Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida |
title_sort |
early molecular immune responses of turbot ( scophthalmus maximus l.) following infection with aeromonas salmonicida subsp. salmonicida |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241612944 https://doaj.org/article/651f8e62b5194fbab60e7a93bee6a89c |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 12944, p 12944 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/16/12944 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241612944 1422-0067 1661-6596 https://doaj.org/article/651f8e62b5194fbab60e7a93bee6a89c |
op_doi |
https://doi.org/10.3390/ijms241612944 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
16 |
container_start_page |
12944 |
_version_ |
1778148807327875072 |