Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa
Abstract Background The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to i...
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ftdoajarticles:oai:doaj.org/article:6514bdbc883646c9a25fcc4efa04a44b 2023-05-15T15:17:07+02:00 Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto 2021-07-01T00:00:00Z https://doi.org/10.1186/s12936-021-03856-2 https://doaj.org/article/6514bdbc883646c9a25fcc4efa04a44b EN eng BMC https://doi.org/10.1186/s12936-021-03856-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03856-2 1475-2875 https://doaj.org/article/6514bdbc883646c9a25fcc4efa04a44b Malaria Journal, Vol 20, Iss 1, Pp 1-12 (2021) Insecticide resistance Intensity Anopheles gambiae Pyrethroids Bendiocarb Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03856-2 2022-12-31T04:28:50Z Abstract Background The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics. Methods Larvae of Anopheles gambiae sensu lato (s.l.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1 × the diagnostic dose, 5 × and 10 × to determine the intensity of resistance in these vectors. Molecular frequencies of the kdr L1014F and ace-1R G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site. Results Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of An. gambiae to permethrin 10 × were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10 × dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the kdr L1014F allele varied between 59 and 83% depending on the sites and species of the An. gambiae complex, while the frequency of the ace-1R G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of An. gambiae sensu stricto (Kisumu strain). Conclusion Anopheles gambiae ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Insecticide resistance Intensity Anopheles gambiae Pyrethroids Bendiocarb Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Insecticide resistance Intensity Anopheles gambiae Pyrethroids Bendiocarb Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
topic_facet |
Insecticide resistance Intensity Anopheles gambiae Pyrethroids Bendiocarb Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics. Methods Larvae of Anopheles gambiae sensu lato (s.l.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1 × the diagnostic dose, 5 × and 10 × to determine the intensity of resistance in these vectors. Molecular frequencies of the kdr L1014F and ace-1R G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site. Results Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of An. gambiae to permethrin 10 × were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10 × dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the kdr L1014F allele varied between 59 and 83% depending on the sites and species of the An. gambiae complex, while the frequency of the ace-1R G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of An. gambiae sensu stricto (Kisumu strain). Conclusion Anopheles gambiae ... |
format |
Article in Journal/Newspaper |
author |
Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto |
author_facet |
Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto |
author_sort |
Casimir Dossou Kpanou |
title |
Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
title_short |
Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
title_full |
Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
title_fullStr |
Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
title_full_unstemmed |
Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
title_sort |
characterization of resistance profile (intensity and mechanisms) of anopheles gambiae in three communes of northern benin, west africa |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03856-2 https://doaj.org/article/6514bdbc883646c9a25fcc4efa04a44b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-12 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03856-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03856-2 1475-2875 https://doaj.org/article/6514bdbc883646c9a25fcc4efa04a44b |
op_doi |
https://doi.org/10.1186/s12936-021-03856-2 |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766347389993484288 |