Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans
Objective: To isolate and evaluate the antimicrobial activity of the active principle(s) from the ethyl acetate (EtOAc) extract of endophytic fungus Colletotrichum gloeosporioides (C. gloeosporioides) isolated from Sonneratia apetala. Methods: Water agar technique was used to isolate the fungus, and...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
2018
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ftdoajarticles:oai:doaj.org/article:64e4c030c5804d60b8b4320ecdf074cb 2023-05-15T15:17:42+02:00 Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans Tauhidur Rahman Nurunnabi Shaymaa Al-Majmaie Ismini Nakouti Lutfun Nahar S. M. Mahbubur Rahman Md. Hossain Sohrab Md. Morsaline Billah Fyaz M. D. Ismail George P Sharples Satyajit D Sarker 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.233183 https://doaj.org/article/64e4c030c5804d60b8b4320ecdf074cb EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=5;spage=350;epage=354;aulast=Nurunnabi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.233183 https://doaj.org/article/64e4c030c5804d60b8b4320ecdf074cb Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 5, Pp 350-354 (2018) endophytic fungus mangrove plants sonneratia apetala kojic acid colletrtrichum gloeosporioides antimicrobial activity Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.233183 2022-12-31T13:47:32Z Objective: To isolate and evaluate the antimicrobial activity of the active principle(s) from the ethyl acetate (EtOAc) extract of endophytic fungus Colletotrichum gloeosporioides (C. gloeosporioides) isolated from Sonneratia apetala. Methods: Water agar technique was used to isolate the fungus, and both microscopic and molecular techniques were used for identification of the strain. Potato dextrose broth was used to grow the fungus in large-scale. Reversed-phase preparative HPLC analysis was performed to isolate the major active compound, kojic acid. The EtOAc extract and kojic acid were screened for their antimicrobial activity against two Gram-positive and two Gram-negative bacteria as well as a fungal strain using the resazurin 96-well microtitre plate antimicrobial assay. Results: The fungus C. gloeosporioides was isolated from the leaves of Sonneratia apetala. Initial identification of the fugal isolate was carried out using spore characteristics observed under the microscope. Subsequently, the ITS1-5.8S-ITS2 sequencing was employed for species-level identification of the fungus C. gloeosporioides. Five litres of liquid culture of the fungus produced approximately 610 mg of a mixture of secondary metabolites. Kojic acid (1) was isolated as the main secondary metabolite present in the fungal extract, and the structure was confirmed by 1D, 2D NMR and mass spectrometry. The EtOAc extract and compound 1 exhibited considerable antimicrobial activity against all tested microorganisms. Whilst the minimum inhibitory concentration (MIC) values from the EtOAc extract ranged between 2.4× 10-4 mg/mL and 2.5 mg/mL, those of kojic acid (1) were between 0.125 mg/mL and 1 mg/mL. The EtOAc extract and kojic acid (1) were most active against Pseudomonas aeruginosa (MIC = 2.4×10-4 mg/mL) and Micrococcus luteus (MIC = 0.125 mg/mL), respectively. Conclusions: The results revealed that the endophytic fungus C. gloeosporioides could be a good source of commercially important kojic acid, which exhibited antimicrobial properties. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 5 350 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
endophytic fungus mangrove plants sonneratia apetala kojic acid colletrtrichum gloeosporioides antimicrobial activity Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
endophytic fungus mangrove plants sonneratia apetala kojic acid colletrtrichum gloeosporioides antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Tauhidur Rahman Nurunnabi Shaymaa Al-Majmaie Ismini Nakouti Lutfun Nahar S. M. Mahbubur Rahman Md. Hossain Sohrab Md. Morsaline Billah Fyaz M. D. Ismail George P Sharples Satyajit D Sarker Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans |
topic_facet |
endophytic fungus mangrove plants sonneratia apetala kojic acid colletrtrichum gloeosporioides antimicrobial activity Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To isolate and evaluate the antimicrobial activity of the active principle(s) from the ethyl acetate (EtOAc) extract of endophytic fungus Colletotrichum gloeosporioides (C. gloeosporioides) isolated from Sonneratia apetala. Methods: Water agar technique was used to isolate the fungus, and both microscopic and molecular techniques were used for identification of the strain. Potato dextrose broth was used to grow the fungus in large-scale. Reversed-phase preparative HPLC analysis was performed to isolate the major active compound, kojic acid. The EtOAc extract and kojic acid were screened for their antimicrobial activity against two Gram-positive and two Gram-negative bacteria as well as a fungal strain using the resazurin 96-well microtitre plate antimicrobial assay. Results: The fungus C. gloeosporioides was isolated from the leaves of Sonneratia apetala. Initial identification of the fugal isolate was carried out using spore characteristics observed under the microscope. Subsequently, the ITS1-5.8S-ITS2 sequencing was employed for species-level identification of the fungus C. gloeosporioides. Five litres of liquid culture of the fungus produced approximately 610 mg of a mixture of secondary metabolites. Kojic acid (1) was isolated as the main secondary metabolite present in the fungal extract, and the structure was confirmed by 1D, 2D NMR and mass spectrometry. The EtOAc extract and compound 1 exhibited considerable antimicrobial activity against all tested microorganisms. Whilst the minimum inhibitory concentration (MIC) values from the EtOAc extract ranged between 2.4× 10-4 mg/mL and 2.5 mg/mL, those of kojic acid (1) were between 0.125 mg/mL and 1 mg/mL. The EtOAc extract and kojic acid (1) were most active against Pseudomonas aeruginosa (MIC = 2.4×10-4 mg/mL) and Micrococcus luteus (MIC = 0.125 mg/mL), respectively. Conclusions: The results revealed that the endophytic fungus C. gloeosporioides could be a good source of commercially important kojic acid, which exhibited antimicrobial properties. |
format |
Article in Journal/Newspaper |
author |
Tauhidur Rahman Nurunnabi Shaymaa Al-Majmaie Ismini Nakouti Lutfun Nahar S. M. Mahbubur Rahman Md. Hossain Sohrab Md. Morsaline Billah Fyaz M. D. Ismail George P Sharples Satyajit D Sarker |
author_facet |
Tauhidur Rahman Nurunnabi Shaymaa Al-Majmaie Ismini Nakouti Lutfun Nahar S. M. Mahbubur Rahman Md. Hossain Sohrab Md. Morsaline Billah Fyaz M. D. Ismail George P Sharples Satyajit D Sarker |
author_sort |
Tauhidur Rahman Nurunnabi |
title |
Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans |
title_short |
Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans |
title_full |
Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans |
title_fullStr |
Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans |
title_full_unstemmed |
Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans |
title_sort |
antimicrobial activity of kojic acid from endophytic fungus colletotrichum gloeosporioides isolated from sonneratia apetala, a mangrove plant of the sundarbans |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.233183 https://doaj.org/article/64e4c030c5804d60b8b4320ecdf074cb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 5, Pp 350-354 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=5;spage=350;epage=354;aulast=Nurunnabi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.233183 https://doaj.org/article/64e4c030c5804d60b8b4320ecdf074cb |
op_doi |
https://doi.org/10.4103/1995-7645.233183 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
5 |
container_start_page |
350 |
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1766347942868811776 |